Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-28T19:35:28.206Z Has data issue: false hasContentIssue false

Chapter 12 - Micromanipulation, Micro-Injection Microscopes and Systems for ICSI

Published online by Cambridge University Press:  02 December 2021

By
Steven D. Fleming
Edited by
Gianpiero D. Palermo  and
Zsolt Peter Nagy
Gianpiero D. Palermo
Affiliation:
Cornell Institute of Reproductive Medicine, New York
Zsolt Peter Nagy
Affiliation:
Reproductive Biology Associates, Atlanta, GA
Get access

Summary

Micromanipulation technology has evolved rapidly over the past 30 years to meet the needs of assisted reproduction practitioners. The clinical outcome of micromanipulation and microinjection procedures is highly dependent upon practitioner skills as well as the quality and reliability of the equipment used. Well engineered mechanical, hydraulic and electronic micromanipulation systems are available and can be mounted upon inverted microscopes supplied by all of the major microscope companies. These systems are complemented by a range of oil and air injectors in addition to anti-vibration tables and lasers. In future, it is possible that some micromanipulation systems will become automated using computer algorithms, enabling robotic procedures to be performed, eliminating variability in practitioner performance.

Keywords

Anti-vibrationIntracytoplasmic sperm injection (ICSI)LaserMicroinjectionMicromanipulationMicroscopePiezo-ICSIRobotic ICSI.
Type
Chapter
Information
Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte
 , pp. 114 - 128
Publisher: Cambridge University Press
Print publication year: 2021

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Malter, H.E. (2016). Micromanipulation in assisted reproductive technology. Reproductive BioMedicine Online, 32, 339–347.CrossRefGoogle ScholarPubMed
Schmidt, H.D. (1864). On the microscopic anatomy, physiology and pathology of the human liver. Confederate States Medical & Surgical Journal, 1, 49–54.Google ScholarPubMed
Fishel, S., Timson, J., Green, S. et al. (1993). Micromanipulation. Reproductive Medicine Review, 2, 199–222.CrossRefGoogle Scholar
Uehara, T. and Yanagimachi, R. (1976). Microsurgical injection of spermatozoa into hamster eggs with subsequent transformation of sperm nuclei into male pronuclei. Biology of Reproduction, 15, 467–470.CrossRefGoogle ScholarPubMed
Palermo, G., Joris, H., Devroey, P. et al. (1992). Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet, 340: 17–18.Google Scholar
O’Neill, C.L., Chow, S., Rosenwaks, Z. et al. (2018). Development of ICSI. Reproduction, 156: F51-F58.CrossRefGoogle ScholarPubMed
Fleming, S.D. and King, R.S. (2003). Micromanipulation in Assisted Conception: A users’ manual and troubleshooting guide – Cambridge: Cambridge University Press.CrossRefGoogle Scholar
Cooke, S. (2016). Low-risk laboratory management. In. Organization and Management of IVF Units: A practical guide for the clinician, ed. Fleming, S.D. and Varghese, A.C., pp. 115–152. Cham: Springer Nature Switzerland AG.Google Scholar
Fleming, S. and Pretty, C. (2019). Research Instruments micromanipulation systems. In. In Vitro Fertilization: A textbook of current and emerging methods and devices, Second Edition, ed. Nagy, Z.P., Varghese, A.C. and Agarwal, A., pp. 455–463. Cham: Springer Nature Switzerland AG.Google Scholar
Joris, H. (2019). Hydraulic micromanipulators for ICSI. In. In Vitro Fertilization: A textbook of current and emerging methods and devices, Second Edition, ed. Nagy, Z.P., Varghese, A.C. and Agarwal, A., pp. 449–454. Cham: Springer Nature Switzerland AG.Google Scholar
Nanassy, L. (2019). Eppendorf micromanipulator: setup and operation of electronic micromanipulators. In. In Vitro Fertilization: A textbook of current and emerging methods and devices, Second Edition, ed. Nagy, Z.P., Varghese, A.C. and Agarwal, A., pp. 465–469. Cham: Springer Nature Switzerland AG.Google Scholar
Hiraoka, K., Kawai, K., Harada, T. et al. (2019). Piezo-ICSI. In. In Vitro Fertilization: A textbook of current and emerging methods and devices, Second Edition, ed. Nagy, Z.P., Varghese, A.C. and Agarwal, A., pp. 481–489. Cham: Springer Nature Switzerland AG.Google Scholar
Lu, Z., Zhang, X., Leung, C. et al. (2011). Robotic ICSI (intracytoplasmic sperm injection). IEEE Transactions On Biomedical Engineering, 58, 2102–2108Google ScholarPubMed
Nagy, Z.P., Liu, J., Joris, H. et al. (1995). The influence of the site of sperm deposition and mode of oolemma breakage at intracytoplasmic sperm injection on fertilization and embryo development rates. Human Reproduction, 10, 3171–3177.Google Scholar
Stoddart, N.R. and Fleming, S.D. (2000). Orientation of the first polar body of the oocyte at 6 or 12 o’clock during ICSI does not affect clinical outcome. Human Reproduction, 15, 1580–1585.Google Scholar
Woodward, B.J., Montgomery, S.J., Hartshorne, G.M. et al. (2008). Spindle position assessment prior to ICSI does not benefit fertilization or early embryo quality. Reproductive BioMedicine Online, 16, 232–238.CrossRefGoogle ScholarPubMed
Saadat, M., Hajiyavand, A.M. and Bedi, A.S. (2018). Oocyte positional recognition for automatic manipulation in ICSI. Micromachines, 9, 429.Google Scholar
Zhang, Z., Dai, C., Huang, J. et al. (2019). Robotic immobilization of motile sperm for clinical intracytoplasmic sperm injection. IEEE Transactions On Biomedical Engineering, 66, 444–452.CrossRefGoogle ScholarPubMed

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×