Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-13T09:22:26.176Z Has data issue: false hasContentIssue false

Chapter 7 - Handling of Gametes and Embryos

Published online by Cambridge University Press:  26 April 2023

By
Sharon T. Mortimer
Edited by
Catherine Racowsky ,
Jacques Cohen  and
Nicholas Macklon
Catherine Racowsky
Affiliation:
Hôpital Foch, France
Jacques Cohen
Affiliation:
IVF 2.0, New York
Nicholas Macklon
Affiliation:
London Women's Clinic
Get access

Summary

This chapter provides an overview of the laboratory systems required for effective handling and processing of spermatozoa, oocytes and embryos for ART. These systems include the laboratory environment, and management of exposure to volatile organic compounds, as well as the culture environment, including temperature, pH, CO2 and O2, osmolarity, culture medium, and contact materials. Other considerations in effective gamete and handling, such as sample identification and witnessing, are also covered.

Type
Chapter
Information
Manual of Embryo Selection in Human Assisted Reproduction , pp. 62 - 69
Publisher: Cambridge University Press
Print publication year: 2023

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Mortimer, D, Cohen, J, Mortimer, ST, Fawzy, M, McCulloh, DH, Morbeck, DE, et al. Cairo consensus on the IVF laboratory environment and air quality: report of an expert meeting. Reprod Biomed Online. 2018;36:658–74.Google Scholar
Cairo 2018 Consensus Group. “There is only one thing that is truly important in an IVF lab: everything”: Cairo consensus guidelines on IVF culture conditions. Reprod Biomed Online. 2020;40:33–60.Google Scholar
Gardner, DK, Lane, M. Alleviation of the ‘2-cell block’ and development to the blastocyst of CFI mouse embryos: role of amino acids, EDTA and physical parameters. Hum Reprod. 1996;11:2703–12.Google Scholar
Morbeck, DE, Paczkowski, M, Fredrickson, JR, Krisher, RL, Hoff, HS, Baumann, NA, et al. Composition of protein supplements used for human embryo culture. J Assist Reprod Genet. 2014;31:1703–11.Google Scholar
Mortimer, ST, Mortimer, D. Quality and risk management in the IVF laboratory. 2nd ed. Cambridge, UK: Cambridge University Press; 2015.Google Scholar
ESHRE Guideline Group on Good Practice in IVF Labs, De los Santos, MJ, Apter, S, Coticchio, G, Debrock, S, Lundin, K, et al. Revised guidelines for good practice in IVF laboratories (2015). Hum Reprod. 2016;31:685–6.Google Scholar
Mortimer, D. The functional anatomy of the human spermatozoon: relating ultrastructure and function. Mol Hum Reprod. 2018;24:567–92.Google Scholar
Mortimer, D. Sperm preparation methods. J Androl. 2000;21:357–66.Google Scholar
World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: World Health Organization; 2010.Google Scholar
Mortimer, D, Mortimer, ST. Density gradient separation of sperm for artificial insemination. In: Carrell, DT, Aston, KI, editors, Spermatogenesis and spermiogenesis: methods and protocols. Methods in Molecular Biology 927, New York: Springer (Humana Press); 2013. p. 217–26.Google Scholar
ESHRE Special Interest Group Embryology and Alpha Scientists in Reproductive Medicine. The Vienna consensus workshop on laboratory key performance indicators. Hum Reprod Open. 2017(2), doi.org/10.1093/ hropen/hox011, and Reprod Biomed Online 2017;35:494–510 (simultaneous publication).Google Scholar
Björndahl, L, Mortimer, D, Barratt, CLR, Castilla, JA, Menkveld, R, Kvist, U, et al. A practical guide to basic laboratory andrology. Cambridge, UK: Cambridge University Press; 2010. p. 219–25.Google Scholar
Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26:1270–83 and Reprod Biomed Online 2011;22:632–46 (simultaneous publication).Google Scholar
Wale, PL, Gardner, DK. The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction. Hum Reprod Update. 2016;22:2–22.Google Scholar

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×