Book contents
- Immunohistochemistry
- Immunohistochemistry
- Copyright page
- Dedication
- Contents
- Contributors
- Preface
- Chapter 1 Immunohistochemistry Fundamentals
- Chapter 2 Immunohistochemistry Quality Assurance and Quality Control
- Chapter 3 Automation and Robotics with the Leica Bond III
- Chapter 4 Automation and Robotics with the Roche Ventana BenchMark ULTRA
- Overview
- Principle of the BenchMark ULTRA
- BenchMark ULTRA Components
- Liquid Coverslip Technology
- Reagent Access Points
- Manual Antibody Titrations
- Antigen Retrieval on the BenchMark ULTRA
- Localization Antibodies
- Ventana Detection Kits
- Double Labelling with the BenchMark ULTRA
- Ventana Quality Control
- Maintenance on the BenchMark ULTRA
- Conclusion
- Appendix 1 Ventana General Buffers with Corresponding Histology Nomenclature
- Appendix 2 Ventana Immunohistochemistry Reagents with Corresponding Histology Nomenclature
- Appendix 3 Ventana In Situ Hybridization Reagents with Corresponding Histology Nomenclature
- Appendix 4 SATB2 Immunohistochemistry Staining Protocol with OptiView Detection and Diaminobenzidine Chromogen
- Appendix 5 PIN4 Protocol Using OptiView Diaminobenzidine and UltraView Red Detection in a Sequential Dual-Colour Immunohistochemical Stain
- Chapter 5 Automation and Robotics with the Agilent Dako Omnis
- Chapter 6 Immunohistochemistry for Research Applications
- Chapter 7 Troubleshooting Immunohistochemistry
- Chapter 8 Current Status of Immunohistochemistry
- Chapter 9 Immunohistochemistry for Future Applications
- Index
Appendix 5 - PIN4 Protocol Using OptiView Diaminobenzidine and UltraView Red Detection in a Sequential Dual-Colour Immunohistochemical Stain
from Chapter 4 - Automation and Robotics with the Roche Ventana BenchMark ULTRA
Published online by Cambridge University Press: 16 June 2022
- Immunohistochemistry
- Immunohistochemistry
- Copyright page
- Dedication
- Contents
- Contributors
- Preface
- Chapter 1 Immunohistochemistry Fundamentals
- Chapter 2 Immunohistochemistry Quality Assurance and Quality Control
- Chapter 3 Automation and Robotics with the Leica Bond III
- Chapter 4 Automation and Robotics with the Roche Ventana BenchMark ULTRA
- Overview
- Principle of the BenchMark ULTRA
- BenchMark ULTRA Components
- Liquid Coverslip Technology
- Reagent Access Points
- Manual Antibody Titrations
- Antigen Retrieval on the BenchMark ULTRA
- Localization Antibodies
- Ventana Detection Kits
- Double Labelling with the BenchMark ULTRA
- Ventana Quality Control
- Maintenance on the BenchMark ULTRA
- Conclusion
- Appendix 1 Ventana General Buffers with Corresponding Histology Nomenclature
- Appendix 2 Ventana Immunohistochemistry Reagents with Corresponding Histology Nomenclature
- Appendix 3 Ventana In Situ Hybridization Reagents with Corresponding Histology Nomenclature
- Appendix 4 SATB2 Immunohistochemistry Staining Protocol with OptiView Detection and Diaminobenzidine Chromogen
- Appendix 5 PIN4 Protocol Using OptiView Diaminobenzidine and UltraView Red Detection in a Sequential Dual-Colour Immunohistochemical Stain
- Chapter 5 Automation and Robotics with the Agilent Dako Omnis
- Chapter 6 Immunohistochemistry for Research Applications
- Chapter 7 Troubleshooting Immunohistochemistry
- Chapter 8 Current Status of Immunohistochemistry
- Chapter 9 Immunohistochemistry for Future Applications
- Index
Summary
- Type
- Chapter
- Information
- ImmunohistochemistryA Technical Guide to Current Practices, pp. 117 - 119Publisher: Cambridge University PressPrint publication year: 2022