from Part II - Protocols
Published online by Cambridge University Press: 20 January 2022
In recent years, the Tooth Cementum Annulations method has been demonstrated to be highly effective at estimating age at death in permanent teeth. Prior to this study, no validation studies have attempted to use TCA to age deciduous teeth. Deciduous teeth provide unique challenges for anthropologists seeking to use the TCA method. Among these are the constraint that cementum annulations are only countable immediately before and after the root apex closes, and the fact that in healthy teeth the cementum record is destined to be destroyed by rhizolysis, so cementum annulations will only be available in teeth that are surgically extracted, or in teeth from juvenile decedents. From a practical standpoint, we found that methods that had previously yielded good results in permanent teeth worked much less well in deciduous teeth. The use of high magnification, bright-field illumination, and analyzing multiple regions of interest per tooth are all recommended for best results.
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