We give a necessary condition for a two-bridge knot or link S(p,q) to be 2-adjacent to another two-bridge knot or link S(r,s). In particular, we show that if the trivial knot or link is 2-adjacent to S(p,q), then S(p,q) is trivial, that if S(p,q) is 2-adjacent to its mirror image, then S(p,q) is amphicheiral, and that for a prime integer p, if S(p,q) is 2-adjacent to S(r,s), then S(p,q)=S(r,s) or S(r,s)=S(1,0).