Field studies were conducted at the Pontotoc Ridge–Flatwoods Branch Experiment Station in Pontotoc, MS, in 2016 and 2017 to determine sweetpotato crop response to saflufenacil and rimsulfuron/thifensulfuron-methyl. Saflufenacil treatments consisted of a factorial of two rates (25 or 50 g ai ha–1) by three application timings [0, 3, or 6 wk before transplanting (WBP)]. Rimsulfuron/thifensulfuron-methyl treatments consisted of a factorial of two rates (18/18 or 35/35 g ai ha–1) by two application timings (3 or 6 WBP). A nontreated check was included for comparison. Saflufenacil resulted in as much as 20% stunting injury in 2016, but ≤4% crop injury in 2017. Compared to the nontreated check, saflufenacil did not reduce yield of any sweetpotato grade regardless of application rate or timing. Findings from this trial indicate that saflufenacil applied in pre-transplanting burndown and field preparation procedures did not have a negative impact on the subsequent sweetpotato crop and that the current plant-back interval (4 to 5 mo) may be excessive. Applications of rimsulfuron/thifensulfuron-methyl at 35/35 g ha–1 made 3 WBP resulted in significant crop injury but did not reduce yield of any sweetpotato grade. Findings from this trial suggest that rimsulfuron/thifensulfuron-methyl applications up to 35/35 g ha–1 applied at least 6 WBP and 18/18 g ha–1 applied at least 3 WBP had little impact on sweetpotato crop growth and may be a safe preplant burndown option.

Herbicides are the foundation for row-middle weed control in Florida plasticulture production. Paraquat is commonly used as a burndown herbicide, and resistance issues have subsequently developed. Halosulfuron is mixed with PRE and POST herbicides to provide additional control of nutsedge. The objective of the study was to determine glufosinate efficacy on weeds emerging in the row-middle and suitability in mixture with halosulfuron for nutsedge control. For total weed control, the high dose of glufosinate (983 g ai ha–1) gave the highest overall control (98% and 64% at 4 wk after treatment for experiments 1 and 2, respectively), and the low rate of glufosinate (656 g ha–1) (67% and 39%) gave results comparable to paraquat (57% and 44%). The high glufosinate dose and paraquat gave comparable control of Brazil pusley (74% to 77% control). Glufosinate + halosulfuron mixture had lower efficacy on Brazil pusley than halosulfuron + paraquat mixture. Glufosinate application reduced grass densities, whereas paraquat did not. Increasing the glufosinate dose did not further decrease grass densities. Similar trends in grass control were also demonstrated in their respective mixtures. Mixing halosulfuron with glufosinate or paraquat did not provide consistent reductions in nutsedge densities, nor did adding paraquat or glufosinate further reduce densities compared with halosulfuron alone for the 4-wk study period. Both paraquat and glufosinate antagonized halosulfuron and reduced efficacy on nutsedge. Compared to controls, there was a reduction between expected and actual nutsedge control for paraquat and glufosinate (25% and 36%), respectively. For total weed control, glufosinate is a suitable alternative to paraquat for row-middle weed management in vegetable production.

Residual herbicides are routinely applied to control troublesome weeds in pumpkin production. Fluridone and acetochlor, Groups 12 and 15 herbicides, respectively, provide broad-spectrum PRE weed control. Field research was conducted in Virginia and New Jersey to evaluate pumpkin tolerance and weed control to PRE herbicides. Treatments consisted of fomesafen at two rates, ethalfluralin, clomazone, halosulfuron, fluridone, S-metolachlor, acetochlor emulsifiable concentrate (EC), acetochlor microencapsulated (ME), and no herbicide. At one site, fluridone, acetochlor EC, acetochlor ME, and halosulfuron injured pumpkin 81%, 39%, 34%, and 35%, respectively, at 14 d after planting (DAP); crop injury at the second site was 40%, 8%, 19%, and 33%, respectively. Differences in injury between the two sites may have been due to the amount and timing of rainfall after herbicides were applied. Fluridone provided 91% control of ivyleaf morningglory and 100% control of common ragweed at 28 DAP. Acetochlor EC controlled redroot pigweed 100%. Pumpkin treated with S-metolachlor produced the most yield (10,764 fruits ha–1) despite broadcasting over the planted row; labeling requires a directed application to row-middles. A separate study specifically evaluated fluridone applied PRE at 42, 84, 126, 168, 252, 336, and 672 g ai ha–1. Fluridone resulted in pumpkin injury ≥95% when applied at rates of ≥168 g ai ha–1; significant yield loss was noted when the herbicide was applied at rates >42 g ai ha–1. We concluded that fluridone and acetochlor formulations are unacceptable candidates for pumpkin production.

Studies were conducted to determine the tolerance of sweetpotato and Palmer amaranth control to a premix of flumioxazin and pyroxasulfone pretransplant (PREtr) followed by (fb) irrigation. Greenhouse studies were conducted in a factorial arrangement of four herbicide rates (flumioxazin/pyroxasulfone PREtr at 105/133 and 57/72 g ai ha–1, S-metolachlor PREtr 803 g ai ha–1, nontreated) by three irrigation timings [2, 5, and 14 d after transplanting (DAP)]. Field studies were conducted in a factorial arrangement of seven herbicide treatments (flumioxazin/pyroxasulfone PREtr at 40/51, 57/72, 63/80, and 105/133 g ha–1, 107 g ha–1 flumioxazin PREtr fb 803 g ha–1S-metolachlor 7 to 10 DAP, and season-long weedy and weed-free checks) by three 1.9-cm irrigation timings (0 to 2, 3 to 5, or 14 DAP). In greenhouse studies, flumioxazin/pyroxasulfone reduced sweetpotato vine length and shoot and storage root fresh biomass compared to the nontreated check and S-metolachlor. Irrigation timing had no influence on vine length and root fresh biomass. In field studies, Palmer amaranth control was≥91% season-long regardless of flumioxazin/pyroxasulfone rate or irrigation timing. At 38 DAP, sweetpotato injury was≤37 and≤9% at locations 1 and 2, respectively. Visual estimates of sweetpotato injury from flumioxazin/pyroxasulfone were greater when irrigation timing was delayed 3 to 5 or 14 DAP (22 and 20%, respectively) compared to 0 to 2 DAP (7%) at location 1 but similar at location 2. Irrigation timing did not influence no.1, jumbo, or marketable yields or root length-to-width ratio. With the exception of 105/133 g ha–1, all rates of flumioxazin/pyroxasulfone resulted in marketable sweetpotato yield and root length-to-width ratio similar to flumioxazin fb S-metolachlor or the weed-free checks. In conclusion, flumioxazin/pyroxasulfone PREtr at 40/51, 57/72, and 63/80 g ha–1 has potential for use in sweetpotato for Palmer amaranth control without causing significant crop injury and yield reduction.

The objective for this study was to determine if POST-directed applications of flumioxazin reduce fruit yield for chile pepper produced on coarse- and fine-textured soils irrigated by furrow. This objective was addressed with a multiyear (2015, 2016, 2017) field study that compared flumioxazin effects on fruit yield against a commercial standard (POST-directed carfentrazone) and the absence of a POST-directed herbicide. The field study occurred at two university research farms that differed in soil texture. On fine-textured soil, treatments included the no POST–directed herbicide control and the following four POST-directed herbicides applied to raised beds: (1) flumioxazin at 107 g ai ha–1 applied 4 wk after crop thinning, (2) carfentrazone at 35 g ai ha–1 applied 4 wk after crop thinning, (3) flumioxazin at 70 g ai ha–1 applied 4 and 6 wk after crop thinning, (4) carfentrazone at 35 g ai ha–1 applied 4 and 6 wk after crop thinning. On coarse-textured soil, treatments included the no POST–directed herbicide control and the following three POST-directed herbicides applied 4 wk after crop thinning: (1) flumioxazin at 107 g ai ha–1 applied to raised beds, (2) flumioxazin at 107 g ai ha–1 applied to furrows, (3) carfentrazone at 35 g ai ha–1 applied to raised beds. On fine-textured soil, treatment did not affect fruit yield. On coarse-textured soil, flumioxazin applied to furrows did not reduce fruit yield, but flumioxazin on raised beds reduced fruit yield of some cultivars in 2015 and 2017. Year-to-year variability in both flumioxazin-induced yield loss and soil characteristics suggested that chile pepper sensitivity to flumioxazin was negatively associated with soil organic matter content. In a follow-up greenhouse study, soil organic matter lessened flumioxazin-induced crop injury. In general, this study indicates that recommendations for POST-directed flumioxazin in New Mexico chile pepper will need to be soil-type specific.

Evolution and rapid spread of herbicide-resistant (HR) kochia has become a significant challenge for growers in the U.S. Great Plains. The main objectives of this research were to confirm and characterize the response of putative auxinic HR (Aux-HR) kochia accessions (designated as KS-4A, KS-4D, KS-4H, KS-10A, KS-10-G, and KS-10H) collected from two different corn fields near Garden City, KS, to dicamba and fluroxypyr and to determine the EPSPS gene copy number to detect whether those accessions were also resistant to glyphosate. Single-dose experiments indicated that putative Aux-HR kochia accessions had 78% to 100% and 85% to 100% survivors when treated with dicamba (560 g ae ha−1) and fluroxypyr (235 g ae ha−1), respectively. Whole-plant dicamba dose–response studies revealed that the selected Aux-HR accessions had 2.9- to 15.1- and 3.1- to 9.4-fold resistance to dicamba relative to two susceptible accessions (MT-SUS and KS-SUS). In a separate fluroxypyr dose–response experiment, the selected Aux-HR accessions also exhibited 3.8- to 7.3- and 3.0- to 8.6-fold resistance to fluroxypyr on the basis of shoot fresh and dry weight responses, respectively. The confirmed Aux-HR kochia accessions also had 3 to 13 EPSPS gene copies relative to MT-SUS and KS-SUS accessions (each with 1 EPSPS gene copy). These results suggest that the putative Aux-HR kochia accessions from Kansas had developed moderate to high levels of cross-resistance to dicamba and fluroxypyr and low to high levels of resistance to glyphosate. This is the first confirmation of kochia accessions with cross-resistance to dicamba and fluroxypyr in Kansas. Growers should use diverse kochia control programs, including the proper use of dicamba and fluroxypyr stewardship, use of cover crops, occasional tillage, diversified crop rotations, and alternative effective herbicides to prevent further evolution and spread of Aux-HR kochia on their fields.

Broadleaf infestations interfere with Florida strawberry production. Broadleaf POST herbicide options applied atop the crop are limited to synthetic auxins and not suitable for conventional multi-cropping and organic systems. Reducing light access and interception during weed emergence may reduce interference. Light-limited growth of two problematic broadleaves, black medic and Carolina geranium, and the most commonly grown strawberry cultivar (‘Florida Radiance’), were examined in the greenhouse. The experimental design was completely randomized, and the trial was repeated. Black medic was susceptible to reductions in incoming solar radiation, wherein reducing the daily maximum available light from 331 to 94 µmol m−2 s−1 reduced leaf number and area by 93% and 89%, respectively. Carolina geranium growth was less susceptible to reduced-light treatments, with leaf area and number each reduced by 66% when light was reduced from 331 to 94 µmol m−2 s−1. Belowground, Carolina geranium biomass was similarly reduced between the 331 and 94 µmol m−2 s−1 treatments. Strawberry was relatively tolerant to shading at 155 µmol m−2 s−1, but further reductions did increase mortality. Shade-induced weed suppression is a promising alternative strategy for conventional and organic Florida strawberry production. Targeted application during periods of weed emergence may play a role within integrated pest management strategies. This approach is most feasible for black medic management but may be useful for Carolina geranium in concert with other strategies.

Glyphosate-resistant (GR) horseweed is one of the most common and troublesome weeds in soybean production fields in several states in the United States, including Nebraska. The evolution of horseweed resistant to several herbicide sites of action has prioritized an integrated approach, including tillage, for effective management of this problem weed. The objectives of this study were to evaluate the effect of tillage or herbicide applied in fall or spring followed by a PRE, POST, and PRE followed by a POST herbicide program for GR horseweed control as well as GR soybean injury and yield in Nebraska. Field studies were established in the fall 2014–2015 and 2015–2016 growing seasons using a factorial randomized complete block design with shallow tillage or herbicide applied at different timings as two factors. Shallow tillage was accomplished using a 50-cm-wide rototiller operated at a depth of 10 cm. At soybean harvest, tillage applied the previous year in fall or spring without any follow-up herbicide treatment provided 79% to 88% horseweed control compared with 27% and 56% control with 2,4-D plus carfentrazone applied in fall and spring, respectively. Tillage or herbicide applied in fall or spring followed by a PRE, POST, or PRE and POST herbicide provided 82% to 99% GR horseweed control at soybean harvest. Soybean yield in this study was similar in most treatments. Tillage or herbicide applied in fall or spring provided similar horseweed control and soybean yield when followed by a PRE, POST, or PRE and POST herbicide; therefore, fall- or spring-applied herbicides can be rotated with shallow tillage for integrated season-long horseweed management.

Flax yield can be severely reduced by weeds. The combination of limited herbicide options and the spread of herbicide-resistant weeds across the prairies has resulted in a need for more weed control options for flax producers. The objective of this research was to evaluate the tolerance of flax to topramezone, pyroxasulfone, flumioxazin, and fluthiacet-methyl applied alone as well as in a mix with currently registered herbicides. These herbicides were applied alone and in mixtures at the 1X and 2X rates and compared with three industry standards and one nontreated control. This experiment was conducted at Carman, MB, and Saskatoon, SK, as a randomized complete block with four replications. Data were collected for crop population, crop height, yield, and thousand-seed weight. Ratings for crop damage (phytotoxicity) were also taken at three separate time intervals: 7 to 14, 21 to 28, and 56+ d after treatment. Crop tolerance to these herbicides varied between site-years. This was largely attributed to differences in spring moisture conditions and the differences in soil characteristics between sites. Herbicide injury was transient. Hence, no herbicide or combination of herbicides significantly impacted crop yield consistently. Flumioxazin was the least promising herbicide evaluated, as it caused severe crop damage (>90%) when conditions were conducive. Overall, flax had excellent tolerance to fluthiacet-methyl, pyroxasulfone, and topramezone. Flax had excellent crop safety to the combination of pyroxasulfone + sulfentrazone. However, mixing fluthiacet-methyl and topramezone with MCPA and bromoxynil, respectively, increased crop damage and would not be recommended.

Preemergence herbicides are typically applied by broadcasting to the top of raised beds before laying the plastic mulch in plasticulture production systems. Broadleaf and grass emergence is limited to transplant holes in the mulch. As a result, most herbicides are applied under the mulch in locations where weeds cannot emerge and herbicides are unnecessary. To reduce this excessive off-target application, a precision hole-punch sprayer was developed at the University of Florida for use in plasticulture production systems. The technology facilitates the application of herbicides during the hole-punch operation immediately before transplant. Application of napropamide and S-metolachlor in an application volume of 233 L ha−1 of water using the precision hole-punch applicator had no effect on tomato and bell pepper growth and yield. Equipment accuracy ranged from 55% to 90%. Preemergence herbicide use was reduced by 88% to 92% with no reduction in weed control. The hole-punch applicator is an effective way to reduce PRE herbicide use in transplant vegetables grown using the plasticulture production system.

Dicamba-resistant (DR) kochia is an increasing concern for growers in the US Great Plains, including Kansas. Greenhouse and field experiments (Garden City and Tribune, KS, in the 2014 to 2015 growing season) were conducted to characterize the dicamba resistance levels in two recently evolved DR kochia accessions collected from fallow fields (wheat–sorghum–fallow rotation) near Hays, KS, and to determine the effectiveness of various PRE herbicide tank mixtures applied in fall or spring prior to the fallow year. Dicamba dose–response studies indicated that the KS-110 and KS-113 accessions had 5- to 8-fold resistance to dicamba, respectively, relative to a dicamba-susceptible (DS) accession. In separate field studies, atrazine-based PRE herbicide tank mixtures, dicamba + pendimethalin + sulfentrazone, and metribuzin + sulfentrazone when applied in the spring had excellent kochia control (85% to 95%) for 3 to 4 mo at the Garden City and Tribune sites. In contrast, kochia control with those PRE herbicide tank mixtures when applied in the fall did not exceed 79% at the later evaluation dates. In conclusion, the tested kochia accessions from western Kansas had evolved moderate to high levels of resistance to dicamba. Growers should utilize these effective PRE herbicide tank mixtures (multiple sites of action) in early spring to manage kochia seed bank during the summer fallow phase of this 3-yr crop rotation (wheat–corn/sorghum–fallow) in the Central Great Plains.

Palmer amaranth is one of the most problematic weeds in cropping systems of North America, especially in midsouthern United States, because of its competitive ability and propensity to evolve resistance to several herbicide sites of action. Previously, we confirmed and characterized the first case of nontarget site resistance (NTSR) to fomesafen in a Palmer amaranth accession from Randolph County, AR (RCA). The primary basis of the present study was to evaluate the cross- and multiple-resistance profile of the RCA accession. The fomesafen dose-response assay in the presence of malathion revealed a lower level of RCA resistance when compared with fomesafen alone. The resistance index of the RCA accession, based on 50% biomass reduction, ranged from 63-fold (fomesafen alone) to 22-fold (malathion plus fomesafen), when compared with a 2007 susceptible, and 476-fold and 167-fold, respectively, relative to a 1986 susceptible check. The RCA accession was resistant to other protoporphyrinogen oxidase (PPO) inhibitors (i.e., flumioxazin, acifluorfen, saflufenacil) as well as the 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor tembotrione and acetolactate synthase (ALS) inhibitor pyrithiobac sodium. Sequencing of the ALS gene revealed no point mutations, indicating that a target-site mechanism is not involved in conferring ALS-inhibitor resistance in the RCA accession. Of the three PPO-inhibiting herbicides tested in combination with the malathion, saflufenacil resulted in the greatest biomass reduction (80%; P < 0.05) and lowest survival rate (23%; P < 0.05) relative to nontreated plants. The application of cytochrome P450 or glutathione S-transferase inhibitors with fomesafen did not lead to any adverse effects on soybean, suggesting a possible role for these compounds for management of NTSR under field conditions. These results shed light on the relative unpredictability of NTSR in conferring herbicide cross- and multiple resistance in Palmer amaranth.

Hydrilla is an invasive aquatic plant that has rapidly spread through many inland water bodies across the globe by outcompeting native aquatic plants. The negative impacts of hydrilla invasion have become a concern for water resource management authorities, power companies, and environmental scientists. The early detection of hydrilla infestation is very important to reduce the costs associated with control and removal efforts of this invasive species. Therefore, in this study, we aimed to develop a tool for rapid, frequent, and large-scale monitoring and predicting spatial extent of hydrilla habitat. This was achieved by integrating in situ and Landsat 8 Operational Land Imager satellite data for Lake J. Strom Thurmond, the largest US Army Corps of Engineers lake east of the Mississippi River, located on the border of Georgia and South Carolina border. The predictive model for presence of hydrilla incorporated radiometric and physical measurements, including remote-sensing reflectance, Secchi disk depth (SDD), light-attenuation coefficient (Kd), maximum depth of colonization (Zc), and percentage of light available through the water column (PLW). The model-predicted ideal habitat for hydrilla featured high SDD, Zc, and PLW values, low values of Kd. Monthly analyses based on satellite images showed that hydrilla starts growing in April, reaches peak coverage around October, begins retreating in the following months, and disappears in February. Analysis of physical and meteorological factors (i.e., water temperature, surface runoff, net inflow, precipitation) revealed that these parameters are closely associated with hydrilla extent. Management agencies can use these results not only to plan removal efforts but also to evaluate and adapt their current mitigation efforts.

Field and greenhouse studies were conducted in 2016 and 2017 to determine sweetpotato tolerance to herbicides applied to plant propagation beds. Herbicide treatments included PRE application of flumioxazin (107 g ai ha−1), S-metolachlor (800 g ai ha−1), fomesafen (280 g ai ha−1), flumioxazin plus S-metolachlor (107 g ai ha−1 + 800 g ai ha−1), fomesafen plus S-metolachlor (280 g ai ha−1 + 800 g ai ha−1), fluridone (1,120 or 2,240 g ai ha−1), fluridone plus S-metolachlor (1,120 g ai ha−1 + 800 g ai ha−1), napropamide (1,120 g ai ha−1), clomazone (420 g ai ha−1), linuron (560 g ai ha−1), linuron plus S-metolachlor (560 g ai ha−1 + 800 g ai ha−1), bicyclopyrone (38 or 49.7 g ai ha−1), pyroxasulfone (149 g ai ha−1), pre-mix of flumioxazin plus pyroxasulfone (81.8 g ai ha−1 + 104.2 g ai ha−1), or metribuzin (294 g ai ha−1). Paraquat plus non-ionic surfactant (280 g ai ha−1 + 0.25% v/v) POST was also included. After plants in the propagation bed were cut and sweetpotato slip number, length, and weight had been determined, the slips were then transplanted to containers and placed either in the greenhouse or on an outdoor pad to determine any effects from the herbicide treatments on initial sweetpotato growth. Sweetpotato slip number, length, and/or weight were affected by flumioxazin with or without S-metolachlor, S-metolachlor with or without fomesafen, clomazone, and all fluridone treatments. In the greenhouse studies, initial root growth of plants after transplanting was inhibited by fluridone (1,120 g ai ha−1) and fluridone plus S-metolachlor. However, by 5 wk after transplanting few differences were observed between treatments. Fomesafen, linuron with or without S-metolachlor, bicyclopyrone (38 or 49.7 g ai ha−1), pyroxasulfone with or without flumioxazin, metribuzin, and paraquat did not cause injury to sweetpotato slips in any of the studies conducted.

Chloris spp. are warm-season grasses that outcompete crops for scarce resources throughout Australia. In Queensland, mild winters and increased adoption of conservation tillage practices have led to an increase of this warm-season grass family in winter crops. The objective of this study is to understand whether droplet size (nozzle type) effects herbicide efficacy of summer perennial grasses, as previous research found no effect of droplet size (nozzle type) on herbicide efficacy of winter annual grasses. A study to compare droplet-size (nozzle type) effects on control of windmillgrass and its domesticated relative, rhodesgrass, was conducted at the University of Queensland in Gatton, QLD, Australia. Results showed little difference in dry weight reductions for windmillgrass or rhodesgrass across droplet size (nozzle type). Paraquat applications with the TTI nozzle resulted in significantly lower dry weight reductions compared with other droplet-size sprays (nozzle types) for rhodesgrass. Glyphosate, imazamox plus imazapyr, and clodinafop resulted in commercially acceptable control for both species, regardless of the droplet size (nozzle type) selected, indicating droplet size (nozzle type) has relatively little impact on the efficacy of these herbicides. Proper nozzle selection can result in control of Chloris spp., a hard to control weed species, while reducing the occurrence of spray drift to nearby sensitive areas.