Annual applications of three nitrogen (N) rates, two broadleaf herbicides, and six annual-grass herbicides in Kentucky bluegrass (Poa pratensis L.) turf were made from 1974 to 1981. With each increment increase in N (2, 4, and 6 kg N/200 m2), the percentage cover of crabgrass (Digitaria spp.) and number of common dandelion (Taraxacum officinale Weber # TAROF) plants were reduced when compared with the next lower rate. There was an interaction between crabgrass control by the annual-grass herbicides and fertilizer rates. When highly effective herbicides were used, there were no differences in crabgrass control related to N rate. When less effective herbicides or no annual-grass herbicides were used, there were great changes directly related to N rate. Each increment of increased N rate increased the turf quality rating. The broadleaf herbicides resulted in differential quality ratings, although differences were relatively small. Highest quality ratings were for plots treated with the three most effective annual-grass herbicides. Residues in the ppb range of some herbicides were detected in soils sampled about a year after the eighth annual herbicide treatments, but residues were below the phytotoxic level and therefore would have had no effect on control of weeds.

Residue levels of fluridone {1-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone} in soil were determined indirectly by measuring the chlorophyll content of wheat (Triticum aestivum L.) grown on a Norfolk sandy loam (Typic paleudult; fine loam, siliceous, thermic) 14, 22, 39, and 66 days after a preemergence application of the herbicide. The half-life for 0.22 and 0.45 kg/ha of fluridone was estimated to be 18 days. Phytotoxicity increased with increasing pH in newly-treated soils and in soil sampled 14 days after treatment. Higher chlorophyll levels in wheat grown in soil sampled 39 days after fluridone application indicated a decrease in biologically-active herbicide. Soil removed from the field following later cultivation produced small reductions in chlorophyll at pH 4.0 and 5.2, but at pH 7.0 significant fluridone activity was still present in the soil after 66 days. Cultivation may have increased the surface concentration of the herbicide.

Handweeding sugarbeets (Beta vulgaris L. ‘Mono Hy D2’) for 8 weeks after planting prevented sugarbeet yield losses. Weeds invading sugarbeet plots at the two-leaf stage (4 weeks after planting) reduced sugarbeet yields 26%. Cycloate (S-ethyl N-ethylthiocyclohexanecarbamate) caused more visual sugarbeet injury and stand reduction than did ethofumesate [(±)-2-ethoxy-2,3-dihydro-3,3-dimethyl-5-benzofuranyl methanesulfonate]. Broadleaf and grass weeds differed in their response to cycloate and ethofumesate, but overall, total weed yields were lower in plots treated with cycloate. Ethofumesate injured sugarbeets more when applied before planting and incorporated into the soil than when applied preemergence. Herbicides applied when sugarbeets had four to six leaves generally injured sugarbeets less, but controlled weeds less effectively, than when sugarbeets had two to four leaves. None of these herbicide treatments were as effective as handweeding in providing season-long weed control. Every 1120 kg/ha of oven dry weeds present in sugarbeet fields corresponded to a decrease in sugarbeet root yields of 10 000 to 11 500 kg/ha.

The level of genetic variation for tolerance to herbicides was quantified in populations of slender wild oat (Avena barbata Brott. # AVEBA), wild oat (Avena fatua L. # AVEFA), and godetia (Clarkia williamsonii Lewis & Lewis) that had not been previously exposed to herbicides. Seedlings of wild oat and godetia were treated with barban (4-chloro-2-butynl-m-chlorocarbanilate) and bromoxynil (3,5-dibromo-4-hydroxybenzonitrile), respectively. The plants were rated for phytotoxic effects following treatment. A one-way analysis of variance on arcsin-transformed phytotoxicity ratings showed significant amounts of inter- and intrapopulation variability for herbicide reaction. Furthermore, the amount of genetic variance for herbicide reaction is higher than expected on the basis of mutation alone, suggesting selection favoring genes conferring herbicide tolerance occurs in natural populations.

The absorption, translocation, and metabolism of the methyl ester of diclofop {2-[4-(2,4-dichlorophenoxy)phenoxy] propanoic acid} by three sunflower (Helianthus annuus L.) lines was determined at 10 and 30C. Sunflower absorbed up to 27% more 14C-diclofop while growing at 30C and 90 ± 5% relative humidity (RH) than at 10C and 40 ± 10% RH. Absorption of diclofop by susceptible (170415) and tolerant (296292 and Hybrid 894) sunflower lines was similar. Translocation of 14C out of the treated sunflower leaf was greater at 30 (1.3%) than 10C (0.5%) 192 h after treatment when averaged across sunflower lines. The diclofop-susceptible line exported more of the applied diclofop from the treated leaf to the shoot portion below the treated leaf than the tolerant lines. Metabolism of diclofop was more rapid at 30 than 10C; however, the three sunflower lines metabolized diclofop similarly.

The dimethylamine salt of 2,4-DB [4-(2,4-dichlorophenoxy)butyric acid] combined with toxaphene (chlorinated camphene) or acifluorfen [5-(2-chloro-4-(trifluoromethyl)phenoxy)-2-nitrobenzoic acid] and toxaphene combined with acifluorfen at several rates each were applied in the greenhouse and field to sicklepod (Cassia obtusifolia L.) to determine interactions. Toxaphene plus 2,4-DB treatments produced synergistic or additive responses in the greenhouse and in the field. Antagonistic or additive responses were observed with acifluorfen plus 2,4-DB treatments in the greenhouse, but not in the field. Acifluorfen plus toxaphene produced only additive responses in the greenhouse with synergistic interactions observed under field conditions.

Dicamba (3,6-dichloro-o-anisic acid), 2,4-D [(2,4-dichlorophenoxy)acetic acid], and picloram (4-amino-3,5,6-trichloropicolinic acid) were applied as simulated spray drift to sugarbeets at several growth stages. When applied at early growth stages 2,4-D tended to decrease root yield but decreased purity and extractable sucrose content by as much as 54% when applied at later growth stages. Dicamba at 0.14 kg ae/ha and 2,4-D at 0.28 kg ae/ha decreased extractable sucrose/ha and tended to decrease root yield at harvest while picloram at 0.028 kg ae/ha did not significantly reduce root yield or sucrose content at harvest compared to the control. All rates of 2,4-D from 0.035 to 0.28 kg ae/ha increased sucrose losses during post-harvest storage at 5 C and 95% relative humidity. Dicamba and picloram at 0.14 and 0.028 kg ae/ha, respectively, caused similar storage losses. Sugarbeets that are inadvertently exposed to 2,4-D, dicamba, or picloram spray drift during the growing season should be processed immediately after harvest.

Experiments were conducted in the field, greenhouse, and controlled environmental chambers to determine the injury to sunflower (Helianthus annuus L.) from diclofop (2-[4-(2,4-dichlorophenoxy) phenoxy] propanoic acid} as influenced by diclofop rate, sunflower line and growth stage, and temperature/relative humidity (RH). Eighteen sunflower lines varied widely in response to diclofop applied postemergence at 2.2 kg/ha with visual injury ratings ranging from 5 to 46%. Neither diclofop-tolerant (Hybrids 894 and 903) nor diclofop-susceptible (Hybrid 923 and Inbred HA290) sunflower lines were injured by diclofop at 10 C. Diclofop injured all four sunflower lines at 30 C. Sunflower injury following diclofop application at 30 C was greater at 90% RH than at 40% RH; and the diclofop-susceptible lines were injured more than the diclofop-tolerant lines. Diclofop injury to sunflower at the two- to four-leaf stage was greater than at any other growth stage in the field and greenhouse. Seed yields of sunflower (Hybrid 894) treated with diclofop in the two- and four-leaf stage was reduced 15 and 12% compared to that of untreated weed-free sunflower, respectively, averaged over diclofop rates.

Five field experiments were conducted with snap bean (Phaseolus vulgaris L.) row spacings of 15, 25, 36, 46, and 91 cm, all of which were seeded at a constant density of 43 plants/m2. When weeds were allowed to emerge with the crop, row spacings of 15 to 36 cm suppressed weed growth by 18% compared to the conventional 91-cm row spacing. When weeds were controlled for the first half of the season, row spacings of 15 to 36 cm suppressed weed growth by 82% compared to the 91-cm row spacing. The effect of the 46-cm row spacing on weed growth was variable. Weed suppression by narrow rows was explained by a higher rate of snap bean canopy closure in narrow rows. Snap beans in row spacings of 15 to 46 cm produced similar yields and were higher by an average of 23% than yields produced by snap beans in 91 - cm rows.

Wild oat (Avena fatua L.) seedlings from mature seeds emerged in silt loam from depths up to 17.5 cm in the greenhouse or 15 cm under field conditions. No emergence occurred from greater depths. Under field conditions, the greatest percentage of shoots emerged from seeds planted 5 cm deep. Wild oat in a space-planted nursery produced an average of 19 tillers, reached a maximum height of 79 cm, and produced an average of 1072 seeds. The number of tillers per plant, the number of seeds per panicle, and the total number of seeds per plant increased with an increase in annual precipitation. Wild oat seeds harvested as early as 3 days after anther extrusion germinated, but seeds harvested 6 or 7 days after anther extrusion germinated more rapidly.

Corn (Zea mays L. ‘DeKalb 640′), kidney beans (Phaseolus vulgaris L. ‘Light red’) and oats (Avena sativa L. ‘Kanota’) were grown in solution culture and the roots exposed to radioactively labeled atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine]. The radioactivity from 3H-atrazine, or its metabolites, was located in microautoradiographs of corn leaf sections, almost exclusively at the perimeter of the vascular bundles, and was primarily confined to the cell walls and intercellular spaces, with no activity associated with subcellular organelles. Radioactivity from 3H-atrazine in oat leaf section microautoradiographs was primarily associated with the chloroplasts, with no evidence of radioactivity specifically associated with other subcellular organelles. Sucrose density gradient centrifugation of homogenized leaf tissue following exposure of the plants to 14C-atrazine showed that most of the radioactivity was associated with the light cell fragments and cytoplasm for the test species. There was no accumulation of 14C-activity in any of the heavier subcellular organelles from corn leaf cells. A peak of radioactivity was associated with the chloroplast fraction from oat and kidney bean leaves.

In 1979 and 1980, ivyleaf morningglory [Ipomoea hederacea (L.) Jacq. # IPOHE] seed were planted in field plots at monthly intervals beginning with March and continuing through October. During the first 9 weeks, seedlings from the July plantings grew more rapidly than those from the other monthly plantings and produced the heaviest fresh weights. Vegetative growth slowed after the 9-week harvest and seedlings from June plantings had heavier fresh weights at the 12-week harvest. Plants from the July planting produced the greatest number of seed at both the 9- and 12-week harvests. After scarifying, seed produced by plants from April through August (12-week harvest) germinated best with the 27/32 C night/day air temperature regime, but also germinated at 15/21, 21/27, and 27/32 C regimes. Ivyleaf morningglory seed did not germinate at an air temperature regime of 10/15 C. With an air temperature regime of 32/38 C the germination of some lots began to decrease.

Controlled burning during winter reduced densities of common goldenweed [Isocoma coronopifolia (Gray) Greene] by 33 to 44% and suppressed canopy cover and height of surviving common goldenweeds for 2 yr. Applied to an unburned infestation, 2,4-D [(2,4-dichlorophenoxy) acetic acid] or dicamba (3,6-dichloro-o-anisic acid) granules only partially controlled common goldenweed at rates of 2 kg/ha or less. Tebuthiuron {N-[5-(1,1-dimethylethyl)-1,3,4-thiadiazol-2-yl]-N,N′-dimethylurea} or picloram (4-amino-3,5,6-trichloropicolinic acid) pellets provided excellent control of common goldenweed if applied at 2 kg/ha during March and at 1 kg/ha if applied after burning in February. The burning pretreatment appeared to synergistically enhance effectiveness of herbicides applied at relatively low rates. Increases in standing crop of buffelgrass (Cenchrus ciliaris L.) following burning were usually small and temporary, but effective herbicide treatments and burn-herbicide combinations increased buffelgrass standing crop by as much as three-fold.

In 1979 and 1980, isolates of Alternaria were obtained from diseased leafy spurge (Euphorbia esula L.) plants. The pathogenicity of an Alternaria sp. towards leafy spurge was demonstrated in glasshouse and limited field studies, and the Alternaria is considered to be a potential biological - control agent for leafy spurge. The pathogenicity and cultural characteristics of the fungus suggest its classification as A. tenuissima f. sp. euphorbiae, with isolate Krupinsky 80-3547ss4 (= E. G. Simmons 35-079) as a representative strain.

Because adsorption is a major factor controlling herbicide persistence, activity, and mobility in soils, an extensive literature exists on the measurement of this process. The adsorption data are usually fitted to the Freundlich equation, but attempts to interpret this equation theoretically have had only limited success. A meaningful thermodynamic interpretation of the Freundlich equation can be developed using a proposed standard state for adsorbed herbicide, which assumes that soil organic matter forms a solid solution with the herbicide. When herbicide vapor pressures and aqueous solubilities are taken into account, the losses in free energy of different herbicides, on adsorption onto wetted soil surfaces, are shown to be similar to each other and are slightly less than the loss on absorption to the solid herbicides themselves. The Freundlich exponent is related in a simple way to the decreasing energy of available soil sites as the amount of herbicide adsorbed increases, but this change is relatively small over the range of concentrations used in adsorption experiments. Sample results are given for six triazine, three urea, and one uracil herbicides.

Scarification of freshly harvested redweed (Melochia corchorifolia L.) seed for 15 to 30 s in an electric scarifier equipped with 100- to 120-grit emery cloth resulted in the best germination. Scarified seed germinated best at 35 and 40C. At 10 and 45C there was no germination even after 7 days. Seven days after planting in silt loam soil there was 80 to 90% emergence of redweed from depths of 1 to 5 cm, whereas no emergence was observed from depths of 0, 8, or 10 cm.

The allelopathic potentials of Hope white lupine (Lupinus albus L.) herbage and herbage extracts were evaluated. The emergence and growth of cotton (Gossypium hirsutum L. ‘Deltapine 45A’), soybean [Glycine max (L.) Merr. ‘Lee 68′], and six weed species under greenhouse conditions were measured after seeding directly into a moist, silt loam soil into which dry Hope lupine herbage, collected at second bloom, had been incorporated at rates from 500 to 8000 ppm (dry herbage/dry soil, w/w). At the lower rates, incorporated Hope white lupine stimulated the emergence of large crabgrass [Digitaria sanguinalis (L.) Scop.]; at the higher rates, cotton emergence was inhibited. Cotton and soybean fresh weights were stimulated at incorporation rates up to 2000 ppm, but were inhibited at higher concentrations. The growth of johnsongrass [Sorghum halepense (L.) Pers.] and large crabgrass was stimulated at most incorporation rates. Stimulated plants were chlorotic and had longer internodes. Emergence and growth of other species tested were not significantly affected by incorporated Hope white lupine. Equivalent concentrations of Hope white lupine herbage extracts were about 17 times more inhibitory to germination of cress (Lepidium sativum L. ‘Curlycress’) seed than to growth of sorghum [Sorghum bicolor (L.) Moench ‘AKS 663′] coleoptiles or radicles. The inhibitory principle extracted from Hope white lupine herbage was non-volatile, heat insensitive, and significantly less active in soil. Fractionation of a herbage extract suggested that neither the alkaloid nor phenolic acid content could account for herbage phytotoxicity in soil.

The kinetics of chlorophyll (Chl) fluorescence was used as a tool for detecting herbicide inhibition in studies using intact soybean [Glycine max (L.) Merr.] leaves. The terminal level of fluorescence (FT), obtained 150 s after the onset of illumination of the abaxial leaf surface, was found to be independent of the dark preadaptation interval and to vary little between leaflets and leaves within and among untreated plants. Increases in FT were detected in plants following the foliar application of herbicides which inhibit photosynthetic electron transport. Fluorescence measurements indicated significant electron transport inhibition in leaves following treatment with 40-mM solutions of either atrazine [2-chloro-4-(ethylamino)-6-(isopropyiamino)-s-triazine] or diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] after 0.5 and 1 h, respectively. Results of this study indicate that Chl fluorescence can be used to measure injury qualitatively by photosynthetic electron transport-inhibiting herbicides in intact plants long before visual symptoms of injury occur. Possible uses of this sensitive, rapid, and non-destructive technique for studying herbicide penetration as affected by adjuvants and environmental factors are discussed.

Metribuzin [4-ammo-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one] at 0.4 kg ai/ha controlled rape (Brassica hitra Moench. # SINAL) when applied 2.5 to 10 cm deep in a horizontal layer or in lines 1 to 4 cm apart. Trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) at 0.8 kg ai/ha did not control ‘Rox Orange’ forage sorghum [Sorghum bicolor (L.) Moench. # SORVU] satisfactorily when applied in a subsurface layer, in lines, or in vertical bands 1, 2, and 3 cm apart up to 10 cm deep. Applications of trifluralin at 1.1 kg ai/ha in vertical bands followed by 0.6 kg ai/ha mixed in the surface 2.5 cm controlled forage sorghum as well as or better than surface application followed by incorporation. The combined subsurface and surface trifluralin placement systems were effective for application depths up to 7.5 cm and band spacings up to 2 cm wide. The development of effective subsurface herbicide injection systems would make the use of herbicides requiring incorporation feasible in reduced tillage crop production systems.

When applied at 0.1 or 0.3 kg/ha, acifluorfen {5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid} did not reduce rough rice (Oryza sativa L. ‘Labelle’) yield, milling yield, seed weight, seed germination, or plant height, regardless of growth stage. Application of 0.6 kg/ha to rice in the boot, late boot, or panicle filling stage reduced yields, but had no effect on plant height, seed germination, or whole milling yield.