The C3 and C4 plant components in extrusa samples from oesophageally fistulated cattle can be estimated by δ13Canalysis. However, contamination with saliva containing bicarbonates could cause bias in the estimation of the C3 or C4 components. Saliva from animals fed C4 and C3 diets had δ13C values of – 4.37/103 and –15.97/103, respectively. Both values showed a large positive shift of c. 10 units relative to the diets, but the difference between feeds was maintained in the saliva. On theoretical grounds, contamination of the ingested feed with saliva should have caused a small (1·3–3·4% legume) negative bias when estimating legume percentage in the diet. This did not occur when saliva of known (PC was added to mixtures of C3 and C4 forages which were dried, ground and analysed for δ13C. It is postulated that CO2 was lost from bicarbonate when saliva was dried in contact with fresh plant material, and so no significant bias was detected. It would appear that the δ13C technique can be used to estimate the proportion of C3 and C4 components in oesophageal extrusa samples without significant bias from salivary contamination.

The life history of the black currant mite, Eriophyes ribis, Nal. is of a simple character from the month of May, when the buds of the current year's growth are entered, till March of the following year. During this time the mites live and multiply in the embryonic leaves producing the abnormal growth familiar to fruit growers as “big-bud.” From the end of March onward, however, the buds so attacked gradually die, and the mites which have lived in them for the past nine months are forced to find other plant food. At this time the buds of the black currant which the mites will eventually live in are rudimentary and it is the end of May before they are sufficiently developed for the acarids to enter.

An experiment in which all combinations of four rates of N fertilizer soon after sowing, four in early spring and nine in late spring were applied to the same plots of winter wheat for 6 years is described and the effects discussed.

It was found that although the yield varied considerably from year to year the regime leading to greatest yield was fairly consistent. If the farmer had used the regime which gave the greatest average yield (0, 90, 180 kg N/ha in autumn, early spring and late spring, respectively) he would have averaged only 0·12 t/ha less than the maximum attainable each year.

Application of N increased protein concentration in the grain more or less linearly but the latest application increased it most. Since the regime which gave greatest yield on average produced grain which qualified for bread-making premium from the protein point of view in all years except 1981, adopting the regime which gave maximum yield would reduce the potential maximum gross margin by only £30/ha.

Analysis of the soil showed a reduction in pH down to 15 cm and of available P2O5 down to 10 cm.

1. Four varieties of marrow stem kale, three of thousand headed kale, Hungry Gap Kale, Rape-Kale and five other rape varieties were sampled at two centres in mid-Wales and separate leaf and stem samples analysed for manganese, molybdenum and boron. Similar samples of four varieties of marrow stem kale, two of thousand headed kale, Hungry Gap Kale, Rape-Kale and two other varieties of rape were taken from two further centres and prepared with the most stringent precautions to prevent contamination. Iron, copper, zinc and lead were determined in these samples.

Bleasdale & Nelder (1960) refer to several models which have been used in studying the relationship of yield and density in crop plants and indicate that they regard as most satisfactory the equation:

where ω = yield/plant, ρ = number of plants/unit area and A and B are constants. The estimation of the constants for any given case is a very unattractive arithmetic exercise requiring a fairly large number of observations and a tedious iterative calculation; Nelder (1961) has discussed the least squares fit with the equation in the form

which may readily be derived from (1) by the substitutions

The data in the present case do not justify attempting this process but some progress can be made by the substitutions

by means of which it is easy to derive from (1) that

1. The age of 210 days was selected for calf weight standardization. In general this is a preweaning weight under prevailing Jamaican conditions, and it represents a compromise between a very early age (90–120 days) which would reflect closely the milking ability of the dam, and the actual weaning age (approximately 260 days) when the grazing management of the herd would constitute a major unpredictable variable.

2. Either one or two weighings taken between the fourth and the ninth months of life was used to standardize calf weights to 210 days of age. In either instance for groups of calves, a mean estimate was derived which was very close to the actual mean 210-day weight, and which was suitable for data relating to groups of animals.

The extent of variation in ratoonability was examined in a range of recently bred short-duration pigeonpea [Cajanus cajan (L.) Millsp.] genotypes in 1989/90, 1990/91 and 1991/92 at the ICRISAT Asia Center, Andhra Pradesh, India. Traits with which this variation could be associated were assessed. Differences in the total ratoon yield of two flushes were significant among the genotypes in each season. In a few genotypes, ratoon yield constituted 50% of the total yield, which could be as high as 5·6 t/ha. Total ratoon yield was positively correlated with leaf area index in the last 2 years of experimentation. The relationship of total ratoon yield to main crop yield was significantly positive in 1991/92 only. The results suggest that there is a large variation in ratoonability among short-duration genotypes and that high leaf area retention at main crop maturity appears to contribute substantially to this variation.

Maize or lucerne silages were fed to two groups of cows in a large dairy herd. Metabolic profile tests carried out during the trial showed that abnormal blood chemistry such as might lead to production disease did not occur in either group.

The results of feed analysis and digestibility trials showed that the two silages provided equal quantities of digestible energy but that the lucerne silage provided more digestible protein than the maize silage. Blood glucose concentrations were similar in both groups of cows but serum urea and albumin concentrations were significantly higher in the cows fed lucerne silage than in those fed maize silage. There was also evidence that lucerne silage provided slightly less available phosphorus but more calcium than the maize silage.

It is concluded that maize silage, as used in these experiments, showed no tendency to lead to production disease, and furthermore the metabolic profile test provided additional information to feed analysis in making this assessment.

1. The object of this investigation was to determine the extent to which the decomposing power of soils within a climatic region is influenced by:

(a) the geological character of the rocks from which the soils are derived,

(b) the nature of the vegetative covering.

2. A short account is given of the geography, climatology, geology and pedology of Glamorgan. The soils of South Glamorgan appear in general to be Brown Earth types.

3. In studying the rate of decay it is necessary to obtain an index of the intensity of the total biochemical processes taking place. One of the best and most suitable of indices for this purpose is CO2 evolution.

4. A type of apparatus used by a previous investigator (Neller) to measure the CO2 evolved from soil was tested and found to be unsatisfactory. Thus a suitable apparatus had to be evolved. This apparatus is described in detail.

The relative unreliability of Bouyoucos's suction method for moisture equivalent determinations is a consequence: (1) of the variation of the suction force maintained with the nature of the soil, (2) of bad reproducibility of the results.

Constant and equal suction for all soils is secured by Schofield's silt-suction method, which may also be made to give fairly well reproducible results. The moisture content at pF 2·9 determined by this method should give a fairly close measure of the moisture equivalent.

While investigating this possibility it was found that the pF curves of stored soil samples may shift considerably with time. In the samples studied the pF at the moisture equivalent has dropped from 2·9 to about 2·4 in 7 years. In view of this shifting the reliability of the silt-suction method for moisture-equivalent determinations has not been definitely ascertained by the experiments reported.

The phenological development from sowing to flowering of the eaxly maize hybrid INRA 200 is related to the weather conditions. Plot trial data from Wytham, near Oxford, England, and weather information from that and nearby sites formed the basic data.

The mean rate of development per day from sowing to emergence is related by linear correlation analysis to the mean values of soil temperature at 5 cm depth and soil moisture deficit. A range of temperature thresholds for emergence development exist, which depend upon the soil moisture, and which differ from the true physiological threshold.

Between omergence and flowering the mean rate of development per day is related by linear correlation analysis to mean air temperature, solar radiation and potential transpiration estimated from weather data. All correlations are significant, but the parameters which combine radiation and temperature are statistically better.

There is currently considerable interest in the use of anabolic compounds to improve the efficiency of animal growth and to produce leaner carcasses. While the majority of work has centred on beef production, the growth performance of sheep is also improved (Galbraith & Topps, 1981). However, little is known of the effects of anabolic steroid hormone administration on wool growth. The few experiments which have been conducted indicate that exogenous oestrogens depress wool output (Slen & Connell, 1958) while exogenous testosterone has consistently enhanced both greasy and clean wool yields (Slen & Connell, 1958; Osborne, 1968; Southcott & Royal, 1971). Little is known, however, of the effects of the synthetic steroids on wool growth. Coehlo, Galbraith & Topps (1981) reported a reduction in wool per unit body weight in sheep implanted with trenbolone acetate and oestradiol-17β, suggesting lowered efficiency of wool growth. Similarly Yasin & Galbraith (1981) noted that trenbolone acetate, in combination with either oestradiol-17β or zeranol, reduced wool output per unit body weight. Neither of these trials attempted to isolate the effects of trenbolone acetate alone from those of the androgenplus-oestrogen combination.

The possible adaptive significance of the occurrence of HK and LK red-cell types in Murrah buffaloes was investigated. Judged by haematological indices and thermorespiratory responses under conditions of moderate thermal stress, LK animals appeared to have a greater adaptive potential than HK animals in a hot arid environment. Dry matter percentage, packed cell volume (pcv), haemoglobin concentration (Hb), red blood corpuscles (RBC) and plasma protein values were significantly higher in LK-type animals. In addition, under identical conditions of thermal stress the LK animals exhibited significantly less rise in rectal temperature and respiratory rate than the HK animals.

Two methods of determining the weight and one method of determining the volume of water imbibed by a soil have been discussed. The results of these methods have been shown to be concordant by an independent method.

The tentative conclusion reached is that the weight of water a soil imbibes can be readily determined by Fisher's method or from the Keen-Raczkowski box, but that the volume of imbibed water cannot yet be directly determined accurately.

P and K fertilizers were applied over a 5-year period to plots that were either cut, individually grazed or communally grazed. Residual effects were assessed in the sixth year when the sward was ploughed and sown with Italian ryegrass. The residual study supported the earlier published inference that (a) P returned to the sward in sheep dung is not an effective P source under field conditions, (b) communal grazing can lead to appreciable transfer of K from one plot to another.

The progenies of six crosses, made in 1987, between the best available hooded barley line and six selected awned barley lines were used to study the effect of the hooded gene on the performance of barley under rainfed conditions in Cyprus. A single dominant gene was found to control the hooded character. Awned genotypes on average outyielded hooded genotypes by 17%, but the difference between the two genotypes was smaller in the central plain than in the coastal area. The effect of the hooded gene on yield varied also with the combination of parents, as the superiority of the awned genotypes varied with cross from 13 to 30%. Hooded lines giving a higher grain yield than their hooded parent were identified. On average the awned genotypes had higher 1000-grain weight (5·0 g), were slightly later in heading date (1·1 days), had slightly higher volume weight (1·3 kg/hl) than hooded genotypes, but had similar plant height. The 1000-grain weight of most hooded lines was significantly higher than that of their hooded parent.

In spite of the negative effects of the hooded gene on grain yield, it was concluded that it is possible to increase the yield of hooded barley for hay production by continued crossing and selection (with special emphasis on 1000-grain weight), by selecting special combinations of parents through the Bulk-Pedigree method and by growing hooded barley in suitable areas, making use of the locationdependent performance of the hooded gene.

Response to selection primarily for lint yield among spaced plant populations of four American and one African variety of Gossypium hirsutum L., established in 1965—6 at Kimberley Research Station, was studied in three experiments conducted between 1966 and 1970.

In the first experiment, in which single-hill micro-plots of 3–5 plants per hill were used, the mean lint yield of progenies was larger than predicted from single plant heritabilities. In the second experiment, in which single-row plots were used, response to selection for lint yield was generally less than expected. In the third experiment, in which three-row plots were used, response to selection for lint yield was again less than expected and less than manifested in the second experiment. However, in all experiments responses to selection for lint percentage, lint quality properties, and height were close to expectations while some individual progenies substantially out-yielded their unselected parents.

The progressive decline in response to selection for lint yield from the first to thethird experiment, and the discrepancies between the actual responses and the expected responses for lint yield in all experiments, were interpreted mainly on a basis of intergenotypic competitive effects differing in sympathy with plot size and plant disposition.

It was concluded that the objective methods of selection practised were successful when judged on plant breeding criteria. It was also concluded that intra-varietal selection is an important technique by which genetic improvement can be achieved at regional breeding centres with limited resources.

Three pot experiments were performed in unheated glasshouses at the University College of North Wales College Farm, Aber, Gwynedd in 1980–1983. Two experiments tested the effects of sowing date and N supply, the third sowing density and nitrogen supply. Extension growth of main-stem leaves was measured by ruler and expressed in thermal time units to allow comparisons between sowing dates. Rate and duration of leaf extension were determined from linear regressions of leaf length against thermal time.

Increasing N supply increased leaf extension rate but had no significant effects on leaf extension duration. Leaf extension rate increased with leaf position on the main shoot, but decreased slightly in leaves extending at the time of stem elongation. Leaf extension duration also increased with leaf position on the main shoot and was related to mean temperature during the leaf extension phase. Plants sown in September were able to compensate for lower radiation receipts by having a faster rate and longer duration of leaf extension, by producing larger leaves with a greater specific lamina area and by partitioning a greater proportion of extension growth into lamina and less into sheaths. In plants sown in June, the largest leaf occurred at a lower stem node and leaves emerging later showed a strong response to N. It is suggested that this is attributable to earlier onset of internal competition for assimilates. Variation in leaf extension rate was the main factor influencing variation in final leaf area. There was a strong positive relationship between leaf extension rate and leaf N content.

Increasing sowing density increased the area of the first four leaves on the main shoot and decreased that of later leaves, changes mainly associated with changes in leaf extension duration. It is concluded that progress in the modelling of leaf area expansion, light interception and dry matter production requires more information about how sowing date, sowing density and N supply interact to influence crop development and leaf growth.