Phenoloxidase (PO) is a significant biomolecule involved in humoral defence mechanism of invertebrates. Spontaneous melanization of insect haemolymph is the major hinderance for studying PO activity, as haemolymph was collected devoid of phenylthiourea. In the study, no visible melanization was observed in crude serum from the grub of Oryctes rhinoceros up to 30 min of incubation amongst crude haemolymph, diluted haemolymph, crude serum and diluted serum that were subjected to visual observation for spontaneous melanization reaction. Accordingly, crude serum was taken for evaluating PO activity. At the same time, as PO substrates tend to auto-oxidize and provide false optical density value, tris-buffered saline devoid of any substrates were used as blank for PO assays. The ideal wavelength at which maximum PO activity occurred for each substrate, namely, tyrosine, tyramine, dopamine, L-dopa, DL-dopa, catechol, protocatechuic acid and pyrogallol was determined as 407, 410, 429, 465, 403, 466, 428 and 400 nm, respectively. Additionally, time course of oxidation for each phenolic substrate by the serum PO were examined and DL-dopa was identified as the specific substrate for serum PO in the grub of O. rhinoceros. Furthermore, maximum PO activity was observed at 5 min of incubation for 10 mM of DL-dopa that was considered as optimum concentration. The ideal pH and temperature for serum PO activity was observed as 7.5 and 20°C, respectively. These results suggested that standardizing a suitable substrate is an essential prerequisite to evaluate the real PO activity of serum which might significantly fluctuate in each insect model.

The action property of an object appears to function as an intrinsic part of its mental representation. This phenomenon has been demonstrated in cases where although the grasp response evoked by a visual object is irrelevant to a participant’s task it still appears to be encoded. This is an affordance effect. Recent findings have shown that dangerous objects can modulate the motor system by evoking aversive affordances. However, the way the time course of response activation generated by the dangerous object develops remains unclear. To investigate this process, we used a priming paradigm that varied the stimulus-onset asynchrony (SOA) between a prime and a target. Participants were asked to judge a symbol after presentation of a dangerous object. Results showed a significant congruency effect between the affordance of the ignored object and the requisite response when the SOA was 800 and 1,200 ms, (t(29) = 4.13, p < .001; t(29) = 2.56, p < .05, respectively). However, with briefer SOAs (0 and 400 ms), this congruency effect was not observed (ps > .10). Results indicate the time course of response activation with a dangerous object are relatively long-lasting.

Visual perception is not instantaneous; the perceptual representation of our environment builds up over time. This can strongly affect our responses to visual stimuli. Here, we study the temporal dynamics of visual processing by analyzing the time course of priming effects induced by the well-known Ebbinghaus illusion. In slower responses, Ebbinghaus primes produce effects in accordance with their perceptual appearance. However, in fast responses, these effects are reversed. We argue that this dissociation originates from the difference between early feedforward-mediated gist of the scene processing and later feedback-mediated more elaborate processing. Indeed, our findings are well explained by the differences between low-frequency representations mediated by the fast magnocellular pathway and high-frequency representations mediated by the slower parvocellular pathway. Our results demonstrate the potentially dramatic effect of response speed on the perception of visual illusions specifically and on our actions in response to objects in our visual environment generally.

The independent and the combined influence of word length, word frequency, and contextual predictability on eye movements in reading was examined across processing stages under two priming-context conditions. Length, frequency, and predictability were used as predictors in multiple regression analyses, with parafoveal, early, late, and spillover eye movement measures as the dependent variables. There were specific effects of: (a) length, both on where to look (how likely a word was fixated and in which location) and how long to fixate, across all processing stages; (b) frequency, on how long to fixate a word, but not on where to look, at an early processing stage; and (c) predictability, both on how likely a word was fixated and for how long, in late processing stages. The source of influence for predictability was related to global rather than to local contextual priming. The contribution of word length was independent of contextual source. These results are relevant to determine both the time course of the influence of visual, lexical, and contextual factors on eye movements in reading, and which main component of eye movements, that is, location or duration, is affected.

The aim of this study was to examine changes in meiotic spindle morphology over time to potentially optimize timing for ICSI. Using polarized light microscopy, images of MII oocytes were captured after retrieval of oocytes in stimulated cycles at six time intervals in culture: 36–36.5 h, 36.5–37.0 h, 38–38.5 h, 39–39.5 h, 40–40.5 h and 40.5–41 h post hCG. Captured images were analysed for spindle presence and their retardance. Results showed that spindles were detected in 58% (45/78) of oocytes at 36–36.5 h. This percentage rose to a peak (96% vs. 58%, p < 0.001) at 39–39.5 h and stabilized between 39–40.5 h post trigger then significantly declined at 40.5–41 h post hCG (96% vs. 77%, p < 0.001). Average spindle retardance increased from 36–36.5 h (1.8 ± 0.7 nm) until it peaked at 39–40.5 h (3.8 ± 0.8 nm, p < 0.0001) and then declined significantly after 40.5–41 h (3.2 ± 0.9 nm, p = 0.0001). These results show that the meiotic spindle appearance is time dependent with the majority of oocytes having detectable spindles and highest retardance between 39–40.5 h post hCG under currently used stimulation protocol after which they start to disaggregate. 39–40.5 h post hCG may be the optimal time for ICSI.