The interaction between cancer cells and the surrounding microenvironment is determinant for metastasis success. In this study, the ultrastructural relevance of cells in the malignant pleural effusion (MPE) of women with breast cancer history was investigated. In MPE, it is possible to observe single cells and clusters. Women whose MPE presents carcinomas in aggregates have a better prognosis when compared to cases in which metastatic single cells are found. Samples were collected via fine-needle aspiration puncture (US-FNA). Subsequent to the material preparation and ultrathin cuts, they were observed using light and transmission electron microscopy (LM/TEM). LM and TEM images served as a basis for the creation of a digital sculpture using ZBrush® software. Clusters exhibited structural stability, en route vesicles allowing exocytosis of electron-dense fibrous elements, and cytoplasmic protrusions contributing to migratory and invasive skills. Single cells presented different necrotic phenotypes and many displayed leukocyte-like characteristics. Cluster cooperative relationships seem to be related to a long-term permanence in MPE. The absence of a collaborative network presumably triggers a more aggressive behavior of single cells. Its putative fusion with leukocytes can maximize the efficiency for transendothelial migration, increasing chances of metastatic success and, unfortunately, reducing survival of women with recidivism.

The Aguablanca stock is a Variscan mafic pluton located in the Ossa-Morena zone, southern Iberian Massif, hosting an unusual Ni–Cu–PGE mineralization associated with magmatic breccia pipes which intruded its northern part. The emplacement of the Aguablanca stock and the mineralized breccia pipes are related to the activity of the Cherneca ductile shear zone, a Variscan sinistral shear zone that favoured magma ascent through the upper crust. A detailed gravity study has been carried out in order to investigate the 3D geometry of the Aguablanca intrusion and to get insights about the emplacement mechanism and tectonic controls of the mineralization. The three-dimensional gravity modelling shows that the stock has an inverted drop geometry with a feeder zone in contact with the Cherneca ductile shear zone. The inferred orientation of the feeder zone suggests that the emplacement probably took place along an open tensional crack formed within the strain field of the adjacent Cherneca ductile shear zone. The modelling of the breccia pipes hosting the Ni–Cu–PGE ore shows that they are included inside the feeder zone, thus their emplacement is probably controlled by successive opening events of this tensional crack.