Differences in habitat complexity and structure can directly influence the composition, diversity, and structure of species assemblages. Measurements of functional and phylogenetic diversity complement the commonly used measurements of taxonomic diversity, elucidating the relationships between species, their traits, and their evolutionary history. In this study, we evaluated how the mosaic of open and forested formations in a federal conservation unit in the western portion of the Brazilian Cerrado savanna influences the taxonomic, functional, and phylogenetic structure of lizard assemblages. Lizards were sampled for 15 months using pitfall traps set in open and forested formations. We recorded 292 lizards distributed among 16 species from eight families, with species composition differing among the formations. Richness was greater in the assemblages from open formations, while functional diversity and phylogenetic variability were greater in those of forested formations. Lizard assemblages in open formations were functionally and phylogenetically clustered, probably as a result of environmental filters acting on species, while the assemblages from forested formations were randomly structured. Different environmental and historical mechanisms have apparently shaped the current diversity of lizards in the region. This study shows that Cerrado vegetation mosaics can promote wide variation in different aspects of the taxonomic, functional, and phylogenetic structure from the lizard assemblages.