The seasonal and bathymetric occurrence of water mites in the sublittoral and profundal zones of lake Banyoles were studied in 1986-1987. Four species were found in the mud substrate : Neumania deltoides (Piersig, 1894), Neumania imitata Koenike, 1908, Unionicola crassipes (Müller, 1776) and Arrenurus sinuator (Müller, 1776). The maximum depth that water mites reached was 12 m, but they were more frequent and abundant at 5 m depth, mainly in the March-May period (41 individuals per m2). This group represents only 0.08-0.39 % of the total macrobenthic fauna in the sublittoral of lake Banyoles. N. deltoides is the most abundant and widely distributed species. For N. imitata this is the first record in the Iberian peninsula.