Recent research in whole-plant stomatal physiology, conducted largely with potted plants in controlled
environments, suggests that stomatal conductance (gs) might be more closely linked to plant chemical variables
than to hydraulic variables. To test this in a field situation, seasonal gs was examined in relation to a number of
plant and environmental variables in 11 temperate, deciduous forest tree species. Stomatal conductance was
generally better correlated with environmental variables (air temperature, vapor pressure deficit, PPFD) than with
plant variables, and slightly better correlated with plant hydraulic variables (shoot water and osmotic potentials)
than with plant chemical variables (xylem sap ABA concentration, xylem sap pH). We examined a model,
developed previously for maize, which describes regulation of gs by xylem sap ABA concentration with leaf water
status acting to modify stomatal sensitivity to the ABA signal. This model explained slightly more variation in
seasonal gs in the forest trees than did single plant variables but not more variation than most single environmental
variables. Response surface models, especially those incorporating environmental variables, were more consistently
successful at explaining gs across species.