Scholars often assume that women's exclusion from the modern civil-religious cargo system in Mexico is a colonial legacy. But an analysis of Chiapas's surviving colonial cofradía books, approximately 200 in all, reveals that formalized female religious leadership was widespread in this region during the colonial period. Close to 50 cofradías in over 20 different towns elected female officials. Indigenous cofradías were clearly at the forefront of this practice; however, it also became remarkably popular among ladino, Black, and even Spanish cofradías during the eighteenth century. Not just symbolic figures, female cofradía officers managed finances and cared for the spiritual and physical welfare of fellow members and the community. Their labors often overlapped with the work of town councils to ensure community well-being and survival in the face of extreme economic exploitation, migration, and forced resettlements. These findings challenge the common generalization, based on studies of central Mexico, that women in colonial New Spain were excluded from officeholding and the prestige and authority it provided. By shifting focus beyond central Mexico, this article illustrates the diversity of female experience and the ways in which gender shaped native communities, cross-cultural exchanges, and dynamic adaptations in religious organizational leadership and Church policy.