A biotype of kochia resistant to chlorsulfuron has been isolated from a Kansas wheat field that had been treated with chlorsulfuron for five consecutive years. In greenhouse tests, the biotype also was resistant to both preemergence and postemergence herbicides with the same mode of action including imazapyr, CGA-131036, the methyl ester of metsulfuron, DPX-M6316, DPX-L5300, and the methyl ester of sulfometuron. The degree of cross resistance varied among the herbicides tested. Herbicides with different modes of action, including atrazine, bromoxynil, MCPA, and diuron, effectively controlled the resistant biotype in the greenhouse.