The growth regulator, glyphosine [N,N-bis(phosphonomethyl)glycine], and other possible metabolites of glyphosine and glyphosate [N-(phosphonomethyl)glycine] [glycine, sarcosine, and aminomethylphosphonic acid (AMPA)] were tested individually (0.5 mM) or as a mixture (each at 0.5 mM) for their effects on growth, extractable phenylalanine ammonia-lyase (PAL) activity, hydroxyphenolic-compound production, chlorophyll and anthocyanin contents, and on soluble-protein levels in soybean [Glycine max (L.) Merr. ‘Hill’] seedlings. Most chemical treatments caused some inhibition of growth either on fresh weight accumulation or on root elongation in the light and dark over 72 h. Glyphosine was generally the most inhibitory and caused the greatest inhibition on axis dry-weight accumulation. Glyphosine significantly increased extractable PAL activity in axes of light- and dark-grown soybeans to a lesser extent than did glyphosate. AMPA had some inhibitory effects on extractable PAL activity whereas other compounds had little influence on the enzyme. These compounds had little effect on total soluble protein in axes or on soluble protein in PAL preparations from 12 to 72 h in light-or dark-grown seedlings. No in vitro effect of the chemicals on PAL activity was found at concentrations up to 0.5 mM. Hydroxyphenolic compound levels increased within 24 to 72 h (per gram fresh weight basis) in light- or dark-grown soybean axes treated with glyphosine, AMPA, or a metabolite mixture (AMPA, sarcosine, and glycine). Anthocyanin content was decreased by glyphosate and to a lesser extent by glyphosine, but was increased by AMPA and the mixture. Glyphosate significantly increased the chlorophyll a/b ratio and decreased total chlorophyll, but glyphosine decreased the chlorophyll content to a lesser degree.

Waterhyacinths grown outdoors were treated with combinations of 0 or 22 kg ai ha-1 of the plant growth retardant paclobutrazol and 0, 25, or 250 waterhyacinth weevils per culture tank under conditions of low (25% coverage) or high (100% coverage) plant densities. The low plant density treatments simulated a regrowth or colonization situation. Weevil feeding activity, canopy height, plant volume, leaf production, and leaf mortality were monitored biweekly for 22 weeks or until all plants had died. In high plant densities, weevil infestations increased leaf mortality rates while decreasing production rates, causing eventual plant death. At low plant densities, waterhyacinth plants partially compensated for weevil feeding by increased leaf production rates, thus enabling them to survive until the end of the study. Paclobutrazol reduced canopy height for about 3 months but had little effect on weevil feeding intensities. Combinations of weevils and paclobutrazol gave complete control of waterhyacinths regardless of plant densities. The combined effects were synergistic, with accelerated leaf mortality rates exceeding production rates leading to early plant death. This study demonstrated the potential for increasing effectiveness of waterhyacinth weevils using integrated strategies.

Glyphosate [N-(phosphonomethyl)glycine] applied at 1.1 kg/ha to wheat [Triticum aestivum (L.) ‘Arthur 71′] plants increased height and fresh weight of soybean [Glycine max (L.) Merr. ‘Ransom′] seedlings planted in the pot at time of application of the glyphosate as the number of wheat plants treated increased from 5 to 30/pot. Height and fresh weight of the soybean seedlings also increased as the rate of glyphosate applied to wheat plants (5/pot) increased from 1.1 to 6.7 kg/ha. Increasing the rate of glyphosate from 1.1 to 6.7 kg/ha, however, reduced the height and fresh weight of soybeans when 30 wheat plants/pot were treated. In addition, when 6.7 kg/ha of glyphosate were applied to wheat plants, soybean-seedling plant height and fresh weight decreased as the density of wheat plants per pot increased from 5 to 30. The 14C-glyphosate exuded into the soil from treated wheat plants was characterized by thin-layer chromatography. Trace amounts of the radio-label were present on thin-layer plates of leaf and stem extracts of corn (Zea mays L.) plants, which were growing in the same pots with the treated wheat plants. The zone of activity had the same Rf value as the glyphosate standard.

A red fescue (Festuca rubra L.)- Kentucky bluegrass (Poa pratensis L.) turf was treated annually with amidochlor {N-[(acetylamino)methyl]-2-chloro-N-(2,6-diethylphenyl)acetamide} at 2.2, 2.8, and 3.4 kg ai/ha, mefluidide {N-[2,4-dimethyl-5-[[(trifluoromethyl)sulfonyl] amino] phenyl] acetamide} at 0.4 kg ai/ha, and ethephon [(2-chloroethyl) phosphonic acid] at 5.6 kg ai/ha for three consecutive years. Recuperative potential of treated turfgrass was determined in the field and in a greenhouse study. Amidochlor and mefluidide treatments injured turfgrass (11 to 64%) four weeks after application. However, turfgrass recovered after eight weeks. Amidochlor at 2.8 to 3.4 kg/ha and mefluidide at 0.4 kg/ha suppressed (75 to 100%) seedhead development. Ethephon at 5.6 kg/ha was ineffective. Turfgrass recovered normally each spring after amidochlor treatments, with no delay in spring green-up. Root length, root weight, and root:shoot weight ratio of the plugs from the greenhouse study were unaffected by three consecutive annual applications of amidochlor, mefluidide, and ethephon. One annual spring application of amidochlor, mefluidide, and ethephon for three consecutive years caused no adverse effects that would limit their use on red fescue-Kentucky bluegrass turf.

Vegetative growth of ‘Tifway’ African bermudagrass (Cynodon transvaalensis Burtt-Davy # CYNTR) x bermudagrass [C. dactylon (L.) Pers. # CYNDA] treated with flurprimidol {α-(1-methylethyl)-α-[4-trifluoromethoxy)phenyl]-5-pyrimidinemethanol} at 0.2 kg/ha and repeated at 3-week intervals for a total rate of 0.8 kg/ha was suppressed for 6 weeks compared to growth of nontreated grass. A single application of flurprimidol at 0.8 kg/ha in late May suppressed growth for 4 weeks. When the single 0.8 kg/ha application was delayed until mid-July, bermudagrass growth was suppressed for only 2 weeks. Applications of nitrogen (N) at 25 and 50 kg/ha did not influence flurprimidol's suppression of bermudagrass growth. Turf quality in some instances was lower when treated with 50 kg N/ha or the turfgrass required a longer recovery period than when treated with 25 kg N/ha following flurprimidol treatment. Although single or multiple applications of flurprimidol, each totaling 0.8 kg/ha, injured bermudagrass in some instances, the injury was not severe and the turfgrass fully recovered.

The effects of the plant growth regulator thidiazuron and the plant pathogen Colletotrichum coccodes on ethylene synthesis by velvetleaf and prickly sida were investigated. Untreated velvetleaf seedlings produced 1.5 and 2.3 pmol ethylene/mg fresh weight 24 and 48 h after treatment. Treatment with thidiazuron at 200 g ai/ha or C. coccodes at 1 × 109 spores/m2 increased ethylene production by 4.9 and 3.5 pmol/mg, respectively, in 24 h; and by 10.6 and 15.8 pmol/mg, respectively, in 48 h. Combination treatments caused synergistic increases in ethylene production by velvetleaf seedlings of 17.7 and 49.6 pmol/mg in 24 and 48 h, respectively. Thidiazuron at 10 μM, combined with an extract of C. coccodes mycelia, increased ethylene synthesis by more than 4-fold in vacuum-infiltrated excised velvetleaf petioles within 48 h. Thidiazuron at 50 g/ha, combined with an extract of C. coccodes mycelium, increased ethylene synthesis 16-fold in prickly sida seedlings in 24 h. Aminocyclopropane carboxylic acid (ACC), a precursor of ethylene, stimulated ethylene production by velvetleaf petioles more than 8-fold when supplied at 0.75 mM; thidiazuron and the pathogen effects were not discernible in its presence. Aminoethoxyvinyl-glycine (AVG), an inhibitor of ACC synthase, inhibited overall ethylene production by velvetleaf seedlings more than 40% when supplied at 0.58 to 0.67 mM, without obscuring the stimulatory effects of thidiazuron and the pathogen. These results indicate that the growth regulator and the plant pathogen or an extract of the pathogen act in concert to increase ethylene synthesis in velvetleaf. Stimulation probably occurs before the synthesis of ACC in the ethylene biosynthetic pathway.

The plant pathogenic fungus Colletotrichum coccodes (Cc) and the plant growth regulator thidiazuron (TDZ) were evaluated in Maryland and Quebec for velvetleaf control in ‘Williams' and ‘Maple Arrow’ soybean. TDZ was applied at 0, 0.1, 0.2, 0.3, and 0.4 kg ai/ha alone or was combined with Cc at 109 spores/m2 when velvetleaf was at the 1- to 2-leaf (Trial 1) or 4- to 6-leaf (Trial 2) growth stages. Velvetleaf control increased with TDZ rate, and TDZ combined with Cc further increased control. TDZ reduced velvetleaf biomass and height, and Cc increased velvetleaf mortality. In Quebec, Cc also reduced the biomass of velvetleaf treated in Trial 1 and interacted positively with TDZ at this growth stage. Cc nearly halved the rates of TDZ required for 90 and 75% mortality of velvetleaf treated at the 1- to 2-leaf and 4- to 6-leaf stages to 0.09 and 0.12 kg/ha, respectively, in Quebec. Cc similarly lowered the rate of TDZ required for 75% stand reduction of velvetleaf in Trial 1 to 0.17 kg/ha in Maryland. Cool wet weather in Quebec contrasted with warm, dry weather in Maryland. Soybean biomass and yield were increased significantly by treatment with TDZ plus Cc in Trial 1 at both locations.

The growth regulator NAA was investigated to determine whether it would control creeping woodsorrel (Oxalis corniculata L. # CYPES) with no phytotoxicity to container ornamental species. Dosage, carrier volume, number of applications, and plant age and growth stage were examined as factors in the phytotoxicity of NAA. Creeping woodsorrel response to the ethyl ester formulation of NAA was linear for dosages up to 5.6 kg/ha; this rate resulted in about 80% injury in all experiments. NAA applied at 8.4 and 11.2 kg/ha achieved 99 and 100% control, respectively. Neither carrier volume nor split applications had any effect on phytotoxicity of NAA. In all experiments, phytotoxicity appeared to increase until around 2 to 3 weeks from application. Phytotoxicity to creeping woodsorrel was related to plant age; as plant age at the time of treatment increased, the level of injury decreased. When plants treated with NAA at rates up to 2.8 kg/ha were cut back to soil level, some regrowth occurred in all cases. Rates of NAA that controlled creeping woodsorrel (5.6 and 11.2 kg/ha) were not phytotoxic to several ornamental species.

The growth of two mallow family weeds, velvetleaf and prickly sida, was inhibited 50% when seedlings were treated with less than 200 g ai/ha of thidiazuron. Spurred anoda was unaffected by treatment with up to 1120 g/ha of thidiazuron. Cotton, okra, and hibiscus, all in the mallow family, also were susceptible to thidiazuron treatment. Leaf development in 2-week-old cotton seedlings was inhibited 20% by less than 35 g/ha thidiazuron as were seedling okra and hibiscus. Plants of all these susceptible species were more resistant when grown for 2 months before treatment. Seedlings of soybean displayed localized injury after treatment, but plant height, biomass, and seed production were not significantly affected 10 weeks after treatment with up to 1120 g/ha thidiazuron. Seedlings of corn appeared unaffected by treatment with up to 1120 g/ha thidiazuron.

Absorption and translocation of the growth regulator 14C-chlorflurenol (methyl-2-chloro-9-hydroxyfluorene-9-carboxylate) and of the herbicide 14C-dicamba (3,6-dichloro-o-anisic acid) were studied in Canada thistle [Cirsium arvense (L.) Scop.]. Also investigated was how foliar treatments with chlorflurenol affected leaf uptake and distribution of dicamba by Canada thistle. Plants were grown in aerated nutrient solution. 14C-chlorflurenol showed strong acropetal and weak basipetal movement. Thirty-two percent of the foliar applied 14C-dicamba was absorbed and most of the radioactivity tended to accumulate in the young growing leaves. Only 0.6% of the applied radioactivity moved to the roots. Leaf absorption and basipetal translocation of 14C-dicamba were increased two- and ten-fold, respectively, by the simultaneous addition of chlorflurenol at 0.6 kg/ha. Root bud activity was increased when 4-week-old Canada thistle plants were foliarly treated with chlorflurenol at 0.6 kg/ha. Field work demonstrated that Canada thistle control could be enhanced by chlorflurenol addition to dicamba.

Goatsrue response to eight herbicide treatments was evaluated in greenhouse and field trials. Herbicides tested on goatsrue grown from seed in the greenhouse included 2,4-D amine, dicamba, chlorsulfuron, picloram, imazapyr, imazamox, aminopyralid, and triclopyr. Each herbicide was applied at rates of 0.125×, 0.25×, 0.5×, 1.0×, and 2.0×, where X is equal to the labeled rate. Goatsrue was most sensitive to the acetolactate synthase inhibitors chlorsulfuron and imazapyr, with 50% inhibition values of 0.07× (3.7g ai ha−1) and 0.16× (90 g ai ha−1) respectively. Goatsrue did not respond to increasing 2,4-D and imazamox rates. Herbicides evaluated in the greenhouse were also tested at two field sites (Smithfield and Amalga, UT), except imazamox, which was replaced by metsulfuron. Field studies gave some varying results, but overall showed that chlorsulfuron, metsulfuron, aminopyralid, and picloram gave at least 93% control at Smithfield, and 89% control at Amalga 24 mo after treatment (MAT). Treatments of chlorsulfuron, metsulfuron, aminopyralid, and picloram were also effective at increasing perennial grass cover at Smithfield 24 MAT. All treatments at Smithfield decreased seedling goatsrue cover, whereas only aminopyralid and picloram decreased seedling cover at Amlaga 11 MAT.

Differential response of wild carrot to 2,4-D was found in seeds collected from 10 locations in Michigan, three in Ohio, one in Illinois, and one in Ontario, Canada. Greenhouse studies were conducted on plants grown from the collected seeds to confirm resistance of wild carrot to 2,4-D, and to study variations among and within populations. The differential response of wild carrot to 2,4-D in field research was due to resistant individuals. Among the 14 locations, wild carrot control with 2,4-D ranged from 18 to 91%. Wild carrot varied in its response to 2,4-D among and within populations as well as within individual umbels. In 69% of the tested samples, at least one wild carrot plant was resistant to 2,4-D.

Applications of aminocyclopyrachlor in 2011 to turf resulted in brown and twisted shoots, leaves, and needles; shoot dieback; and in some cases, death of trees and ornamental plants adjacent to treated turf areas. Our research objective was to determine if a sensitive plant could be injured from wood chips (mulch) obtained from aminocyclopyrachlor-damaged trees, and to quantify movement of aminocyclopyrachlor from contaminated wood chips into soil and its subsequent uptake by roots into landscape plant tissues. Tomatoes were grown under greenhouse conditions and mulched with chipped tree branches collected from honey locust and Norway spruce damaged 12 mo previously by aminocyclopyrachlor. Analysis of tomato tissue for aminocyclopyrachlor residues 32 d after mulching found aminocyclopyrachlor in all mulched tomato plants, which was consistent with observations of epinasty on tomato leaflets. Aminocyclopyrachlor residues ranged from 0.5 to 8.0 ppb in tomato plants while chipped tree branches contained 1.7 to 14.7 ppb. Aminocyclopyrachlor residues in the potting soil below the mulch ranged from below the quantifiable limit to 0.63 ppb, indicating that aminocyclopyrachlor can leach from wood chips into soil, causing plant injury. These results indicate that trees damaged by aminocyclopyrachlor should not be chipped and used for mulch or as an ingredient in compost.

Absorption and translocation of aminocyclopyrachlor (AMCP) was investigated in black walnut. Radiolabeled AMCP was applied foliarly as a free acid, and basally as a free-acid-formulated oil-soluble liquid and as a methyl ester emusifiable concentrate. Maximum absorption was 68% for the methyl ester, 47% for the free-acid oil-soluble liquid, and 8% for the free acid when evaluated as percent applied. When recovered AMCP was presented as Bq g−1, no difference was observed in AMCP absorption or translocation of 14C-labeled material out of treated plant parts. Recovery of applied radiolabeled material in the roots from basal applications was 5 to 11% 72 h after treatment (HAT), compared with 0.8% recovery from a foliar application. Regardless of chemical form or formulation type, comparable injury was observed within 72 HAT despite low levels of translocation.

As the amount of methyl bromide approved for use in Florida strawberry diminishes, growers are faced with a forced transition to alternative fumigants. Many of these methyl bromide alternatives have been associated with reductions in weed control, requiring additional but complementary measures. POST herbicide options for annual strawberry are limited, resulting in significant portions of the strawberry acreage in Florida being hand-weeded when troublesome weeds escape conventional control methods. Strawberry has shown acceptable tolerance to clopyralid in other areas and production systems; however, its integration into the Florida production system and ramifications of applications during fruiting warrants further research. Eight trials were conducted, with three common strawberry cultivars grown in West Central Florida subjected to POST spray and drip-tape-injected applications of clopyralid. Formation of new strawberry leaves was not affected by clopyralid application, except for a reduction in new leaves of the cultivar ‘Strawberry Festival' at the highest rate of application of 261 g ae ha−1 in comparison with the nontreated control. Strawberry leaf malformation was best explained by an exponential growth equation, whereas marketable yield followed the trend of a Weibull peak. At the maximum labeled rate (66 g ha−1), leaf malformation was less than 5% for all cultivars tested, and marketable yield was estimated at 104% of the nontreated control.

Greenhouse experiments were conducted to determine the effect of salinity, imazethapyr, and chlorimuron on weed growth. Five species, barnyardgrass, common cocklebur, ivyleaf morningglory, common purslane, and yellow nutsedge, were grown in potting soil and irrigated with nonsaline (EC ∼ 2 dS m−1) or sulfate-dominated saline (EC ∼ 7 dS m−1) nutrient solution. Plants were treated after emergence with imazethapyr (Pursuit formulation) at 70 g ae ha−1 or chlorimuron ethyl (Classic formulation) at 8.8 g ai ha−1. Results indicated that irrigation with saline water had no overall effect on the growth or survival of most tested weed species. Growth of yellow nutsedge (maximum height and stem diameter) was reduced for plants irrigated with saline water. Observed growth and survival trends in saline and nonsaline treatments were consistent with information on the herbicide label. Complete control of common purslane was not achieved by either chlorimuron or imazethapyr. Growth and survival of ivyleaf morningglory and yellow nutsedge were greater when plants were treated with imazethapyr than when treated with chlorimuron, whereas for barnyardgrass, growth and survival were significantly greater when plants were treated with chlorimuron. Both herbicides affected common cocklebur growth and survival in a similar way. For all tested species, most surviving plants were not vigorous and would not be highly competitive with crop plants. The results of these experiments suggest that weed control information mentioned on the labels for these herbicide formulations will not require modification for moderately saline soils.

In the forest zone of Côte d'Ivoire, Vavoua's traps and supports treated with pyriproxyfen® (Sumitomo) have been tested against Glossina palpalis palpalis. The supports were impregnated one time only at the dosage of 2 mg/cm2.

This juvenile hormone mimic acting as growth regulator has a good efficacy: apparent density was reduced by 87% after 2 months and was maintained at this level for 150 days; percentage of nulliparous females was equal to 0 between 40th and 120th days.

Pyriproxyfen may be recommended for the control of animal trypanosomiasis vectors—if it costs less than the insecticides; its slow effect on tsetse population does not favour its use for the control of human African trypanosomiasis which needs a drastic and immediate reduction of Glossina densities.