A field experiment was conducted in Boone and Callaway counties in Missouri in 2010 and 2011 to investigate herbicide programs for the management of summer annual grass and broadleaf weeds in soybean resistant to 2,4-D and glufosinate. Results revealed that the addition of 0.56, 0.84, or 1.12 kg ha−1 2,4-D (amine) to either or both POST applications of glufosinate in a two-pass POST herbicide program increased control of common waterhemp compared to two POST applications of glufosinate alone. Similar levels of common cocklebur, giant foxtail, large crabgrass, and barnyardgrass control were achieved with any of the two-pass POST programs that contained 2,4-D compared to two-pass POST programs containing glufosinate alone. Similar control of these species was also achieved with the inclusion of 2,4-D in either the first or second pass of glufosinate. Two-pass programs resulted in the highest levels of weed control (90%). Annual grass and broadleaf weed control was as much as 59% lower when one-pass POST herbicide programs were compared to PRE followed by (fb) POST or two-pass POST programs. However, one-pass POST programs were sufficient to obtain exceptional control of common cocklebur and giant foxtail. Across all site years, soybean yields ranged from 2,680 to 3,100 kg ha−1 for all herbicide treatments, but did not differ statistically. Overall, results from these experiments indicate that compared to glufosinate alone, PRE fb POST or two-pass POST herbicide programs that incorporate 2,4-D amine with glufosinate in 2,4-D–resistant soybean enhance control of common waterhemp, while providing similar levels of control of other summer annual grass and broadleaf weeds.