The objective of this systematic review was to evaluate the efficacy of antibiotics to prevent or control colibacillosis in broilers. Studies found eligible were conducted controlled trials in broilers that evaluated an antibiotic intervention, with at least one of the following outcomes: mortality, feed conversion ratio (FCR), condemnations at slaughter, or total antibiotic use. Four electronic databases plus the gray literature were searched. Abstracts were screened for eligibility and data were extracted from eligible trials. Risk of bias was evaluated.

Seven trials reported eligible outcomes in a format that allowed data extraction; all reported results for FCR and one also reported mortality. Due to the heterogeneity in the interventions and outcomes evaluated, it was not feasible to conduct meta-analysis.

Qualitatively, for FCR, comparisons between an antibiotic and an alternative product did not show a significant benefit for either. Some of the comparisons between an antibiotic and a no-treatment placebo showed a numerical benefit to antibiotics, but with wide confidence intervals. The risk-of-bias assessment revealed concerns with reporting of key trial features.

The results of this review do not provide compelling evidence for or against the efficacy of antibiotics for the control of colibacillosis.

The inclusion of fibre has been studied as an alternative to antimicrobials in weaning pig diets, while ZnO is used as an effective method to prevent post-weaning diarrhoea. However, it has not been investigated to what extent these two strategies interact with each other. The present study was designed to evaluate the effects of including wheat bran (WB) and ZnO alone or combined in the diet of early-weaning pigs on productive performance and microbial activity in the gastrointestinal tract (trial 1). A total of sixty-four piglets were distributed in a 2 × 2 factorial combination of two levels of WB (0 v. 40 g/kg) and ZnO (0 v. 3 g/kg) in the diet. The inclusion of ZnO in the diet improved the feed intake and growth of the animals and reduced the incidence of diarrhoea. The inclusion of WB increased SCFA concentrations and decreased Escherichia coli counts. However, simultaneous incorporation of WB and ZnO increased E. coli counts. Two in vitro trials were also designed to clarify hypotheses derived from the in vivo test: (1) the ability of WB and other fibre sources to bind E. coli in vitro (trial 2) and (2) the in vitro interactions between WB and ZnO with respect to E. coli growth (trial 3). We can conclude that incorporation of WB in the diet improved gut health by modulating the activity and composition of the microbial population. The negative interaction between WB and ZnO raises the interest of considering the inclusion of phytase enzymes to reduce the therapeutic levels of ZnO in post-weaning diets.

Response to an experimental infection with an enteropathogenic E. coli O103 strain (EPEC) was assessed in growing rabbits given a high or a low dietary fibre diet (200 and 120 g acid-detergent fibre per kg respectively for high ‘HF’ or low ‘LF’ fibre diet). The two experimental diets, differing in fibre level but not fibre quality, were given ad libitum from 21 days of age to two groups of 12 litters of nine pups, weaned at 28 days (trial 1) and caged collectively. At 42 days of age, the two groups were divided in two subgroups (i.e. four groups of 18 animals), inoculated or not with an EPEC O103 strain. Two further groups of 48 rabbits (trial 2) were given LF and HF diets from 28 days (weaning) to 70 days, and were individually housed to control precisely the food intake. Reducing the fibre level led to a lower post-weaning food intake (64 v. 85 g/days in the period from 28 to 42 days of age, P < 0·001) and digestible energy intake (0·75 v.. 0·86 MJ/day, P < 0·001), causing a lower growth (proportionately 0·088 lower, P < 0·001). At 42 days of age, only 17% of rabbits had a detectable caecal saprophyte E. coli flora (over 102 colony-forming units (CFU) per g, trial 1). Inoculating 6-week-old rabbits with EPEC led to moderate levels of mortality (26%). Health risk index (mortality + morbidity) was numerically higher in LF compared with HF groups (P = 0·12). The acute phase of the colibacillosis was between 3 and 10 days post inoculation. From days 7 to 14 post inoculation, a significantly higher frequency of rabbits having a high faecal excretion of E. coli (> 105 CFU per g) was found in LF than in HF rabbits (respectively 65 v. 26·7%, P = 0·04). The caecal pectinolytic flora reached 108 CFU per g at 42 and 65 days of age, and was not affected by diet or EPEC challenge. In 42-day-old rabbits the caecal volatile fatty acid concentration was higher in HF than in LF groups (respectively 79 v. 60 mmol/l, P < 0·05), while the caecal pH was slightly lower (respectively 5·93 v. 6·09, P < 0·01). At 65 days of age, rabbits resistant to the inoculation showed a similar fermentation pattern and fibrolytic flora level to non-inoculated animals. Caecal ammonia level was not affected either by age, diet or E. coli challenge (mean = 12·1 mmol/l). From these results, we conclude that a high fibre intake improved the resistance of the growing rabbit to a specific enteropathy, in association with a higher caecal fermentative activity and lower caecal pH.