Waterhemp is a dioecious species with wide genetic diversity which has enabled it to evolve resistance to several commonly used herbicide groups in North America. Five field trials were established in Ontario to ascertain the biologically effective doses of diflufenican, a new Group 12 herbicide applied preemergence for control of multiple herbicide–resistant (MHR) waterhemp in corn. Based on regression analysis, the predicted diflufenican doses to elicit 50%, 80%, and 95% MHR waterhemp control were 99, 225, and 417 g ai ha−1, respectively, at 2 wk after application (WAA); 73, 169, and 314 g ai ha−1, respectively, at 4 WAA; and 76, 215, and — (meaning the effective dose was beyond the set of doses in this study) g ai ha−1, respectively, at 8 WAA. The predicted diflufenican doses that would cause a 50%, 80%, and 95% decreases in MHR waterhemp density were 42, 123, and — g ai ha−1; and MHR waterhemp biomass were 72, 167, and 310 g ai ha−1, respectively, at 8 WAA. Diflufenican applied preemergence at 150 g ai ha−1 controlled MHR waterhemp by 64%, 79%, and 73% at 2, 4, and 8 WAA, respectively. Isoxaflutole + atrazine applied preemergence at 105 + 1,060 g ai ha−1 controlled MHR waterhemp by 98%, 98%, and 97% at 2, 4, and 8 WAA, respectively; and S-metolachlor/mesotrione/bicyclopyrone/atrazine applied preemergence at 1,259/140/35/588 g ai ha−1 controlled MHR waterhemp by 100%, 100%, and 99% at 2, 4, and 8 WAA, respectively. Diflufenican applied preemergence reduced MHR waterhemp density and biomass by 83%; in contrast, isoxaflutole + atrazine and S-metolachlor/mesotrione/bicyclopyrone/atrazine reduced MHR waterhemp density and biomass by 99%. All treatments evaluated caused either no, or minimal, corn injury and resulted in corn yield that was similar with the weed-free control. Results indicate that diflufenican applied alone preemergence does not provide superior MHR waterhemp control over the commonly used herbicides isoxaflutole + atrazine or S-metolachlor/mesotrione/bicyclopyrone/atrazine; however, there is potential for using diflufenican as part of an integrated weed management strategy for the control of MHR waterhemp control in corn.

Soil fertility and disturbance can potentially modify weed dynamics in a corn–soybean rotation. Knowing how added mineral fertilizers and tillage influence weed populations in the long term can provide insight into weed community shifts, which may affect future weed management requirements. The first objective of this study was to evaluate the effect of 24 to 25 years of nitrogen and phosphorus fertilization treatments and tillage (moldboard vs. no-till) on weeds (density, biomass, and composition) before and after herbicide applications in 2016/corn and 2017/soybean. The second objective was to evaluate the effect of the same treatments 6 yr after no postemergence weed management in 2022/corn. Since the last evaluation performed in 2004, weed density, richness, and diversity increased, and more annual grasses were observed under both tillage regimes. No fertilization effect was observed on any weed variables, including composition, except for increased biomass when left to grow all season after crop planting. In managed plots, the density and biomass of annuals and perennials were generally higher in no-till, and discrepancies were highest for annual grass densities before herbicide application. Weed species richness and diversity based on counts were equivalent between tillage regimes, but total biomass was distributed between more species in no-till. Higher weed densities and concurrent weed biomass, observed in no-till, reduced crop yields in 2016/soybean only. The combination of low crop diversity and low use of residual herbicides during the trial potentially led to the observed species shifts and increased weed density.

The current study aimed to investigate biofortification of maize grown under different irrigation intervals, i.e. 15, 20 and 25 days (hereinafter referred to as IR15, IR20 and IR25, respectively), using foliar application treatments (silicon (Si), zinc (Zn), silver nanoparticles (AgNPs), Si + Zn, Si + AgNPs, Zn + AgNPs and Si + Zn + AgNPs) in two growing seasons, 2020 and 2021. A split-plot design with four replications was used, where irrigation intervals and foliar treatments were assigned in main plots and subplots, respectively. IR15 received a total of 7925 m3/ha irrigation water divided over seven irrigations, while IR20 received 5690 m3/ha divided over five irrigations and IR25 received 4564 m3/ha divided over four irrigations. The highest yield and grain quality were observed in plants irrigated at 15-day intervals. Spraying the canopy with Si, Zn and AgNPs, either individually or in combination, reduced the negative impact of water stress caused by longer irrigation intervals on plant growth, yield, yield components and grain protein content. In IR15 + AgNPs + Zn, most of the studied parameters, except for proline content, showed a high positive impact, especially on 100-kernel weight (KW). In contrast, IR25 + Si + AgNPs + Zn showed the highest positive effects on proline and protein contents but a negative impact on the harvest index. Collectively, IR15 + Si + AgNPs + Zn resulted in the highest values of all studied parameters, followed by IR15 + Si + AgNPs and IR15 + Si + Zn. In conclusion, our results suggest that an irrigation interval of 15 days combined with application of Si, Zn and AgNPs has the potential to improve yield and quality of maize under water deficit stress.

The development of an integrated weed management (IWM) strategy for control of multiple herbicide-resistant (MHR) waterhemp can provide field crop producers with a strategy to deplete the number of waterhemp seeds in the soil seedbank. Field experiments were established on two commercial farms in Ontario, Canada, with MHR waterhemp in 2017. The number of waterhemp seeds in the seedbank at the Cottam and Walpole Island sites prior to establishing the experiments was 413 and 40 million seeds ha−1, respectively. The goal of this 9-yr study is to document the depletion in the number of waterhemp seeds in the seedbank after Years 3, 6, and 9 (spring 2020, 2023, and 2026) and to identify management practices that can reduce the number of waterhemp seeds by 95% or more. Relative to the number of seeds in the soil seedbank when the experiment was initiated, at the Cottam site after 3 yr of this experiment, in the “control” treatment (continuous soybean seeded in rows spaced 75 apart, and sprayed with glyphosate) there was a numeric 31% increase in the number of waterhemp seeds in the seedbank; in contrast, in the three-crop rotation of corn/soybean/winter wheat (with or without a cover crop after winter wheat harvest), soybean seeded in rows spaced 37.5 cm apart, with herbicide applications using a total of eight different herbicide modes of action resulted in a 65% to 66% decrease in the number of waterhemp seeds in the soil seedbank. At the Walpole Island site after 3 yr of this experiment, the number of waterhemp seeds in the seedbank was not affected by the IWM programs evaluated. Results indicate that a diversified integrated waterhemp management program dramatically decreased the number of waterhemp seeds in the seedbank at one of two sites.

Intercropping maize (Zea mays L.) with cover crops (Crotalaria spectabilis and Urochloa ruziziensis) is a sustainable cultivation strategy that can generate ecological benefits combined with grain yield (GY). However, cover crops may require nitrogen (N) fertilization management to be adjusted to obtain a high GY in intercropping systems. This study was carried out over 2 years in southeastern Brazil using randomized complete block design in a split-plot scheme. The plots were composed of three cropping systems: sole maize, maize + U. ruziziensis and maize + C. spectabilis. The subplots consisted of four N levels: 0, 70, 140 and 210 kg/ha. The GY of the maize + U. ruziziensis may be reduced by 13% compared to the sole maize. The GY of the sole maize increased up to 11.3 Mg/ha for 95 kg/ha of N in the first year and 6.7 Mg/ha for 169 kg/ha of N in the second year. The GY of the maize + U. ruziziensis intercropping system showed linear increments of 120 kg/ha for every 10 kg/ha of N applied. In the maize + C. spectabilis intercropping, GY increased up to 9.8 Mg/ha for 201 kg/ha of N in the first year, without any variation during the second year. The land equivalent ratio (LER) of intercropping was increased by more than 35% compared to that of sole maize, and N fertilization increased the LER of the sole maize and maize + U. ruziziensis intercropping. Maize intercropping had higher LER values with greater demand for nitrogen to obtain similar yields compared to sole maize cropping.

Drought stress and weed competition are two of the most important threats to corn production in the northeastern United States. Both pressures have the potential to worsen under climate change. In a 2-yr field study in Ithaca, NY, we tested the effects of drought and burcucumber, an increasingly problematic annual vine, on silage corn. Burcucumber seedlings were transplanted into corn rows at densities of 0, 0.5, 2, and 3 plants m−2 and a drought treatment was later imposed with rainout shelters constructed from steel frames and high-clarity plastic. Available soil moisture was lower in drought plots (47% ± 1% in 2018 and 52% ± 2% in 2019) than no-drought plots (69% ± 1% in 2018 and 68% ± 1% in 2019). Burcucumber planting density (P = 0.008) reduced fresh silage yield. Drought also reduced fresh silage yield (P < 0.001) with a drought-by-year interaction (P = 0.007): drought reduced fresh weight by 29% in 2018 (48,000 ± 2,000 kg ha−1 to 34,000 ± 3,000 kg ha−1) and by 9% in 2019 (38,000 ± 3,000 kg ha−1 to 34,000 ± 3,000 kg ha−1). Burcucumber planting density and drought did not interact. Overall, our findings indicate that drought and competition from burcucumber may have additive effects on silage corn in New York State. Regardless of water availability, active weed management is required to prevent yield losses due to burcucumber. Yield losses may be similar or greater in grain corn and might increase under climate change.

This study consisting of six field experiments was conducted in 2018 and 2019 to evaluate the tolerance of four corn hybrids (P9998AM, P9840AM, DKC42-60RIB, and DKC43-47RIB) to the tank mix of tolpyralate + atrazine with a commercial glyphosate formulation. At 1 wk after application (WAA), two corn hybrids (P9998AM and P9840AM) exhibited more injury from tolpyralate + atrazine (2× rate) applied alone and in combination with glyphosate than DKC42-60RIB and DKC43-47RIB; however, all corn hybrids responded similarly with respect to visible injury 2, 4, and 8 WAA, stand loss, and yield. Application of tolpyralate + atrazine or glyphosate + tolpyralate + atrazine at the 2× rate caused greater corn injury (up to 27%) than tolpyralate + atrazine or glyphosate + tolpyralate + atrazine at the 1× rate (up to 8%) at 1 WAA. The addition of commercial glyphosate with tolpyralate + atrazine did not enhance injury symptoms. Results of this study show that there is a wide margin of corn safety with tolpyralate + atrazine applied alone and in combination with a commercial formulation of glyphosate.

Tropical soils tend to harden during drying due to the generally low content of free-iron and organic carbon, combined with high fine sand and silt proportions. It was hypothesized that the change in soil physical condition induced by the addition of a leguminous mulch in cohesive tropical soil enriched with calcium may mitigate soil hardening through wetting and drying cycles by rain or irrigation, thereby improving the soil rootability. A leguminous mulch was added in different concentrations to a structurally fragile tropical soil enriched with calcium, which then had different irrigation intervals. The treatments were with or without mulch (10 t/ha), with or without added nitrogen (100 kg/ha at 2 intervals) and two irrigation intervals. In 2015 the irrigation intervals were either 4 or 8 days, and in 2016 they were either 6 or 9 days. Two years were used in the attempt to achieve greater differences, as for tested variables, between treatments. Maize planted in these soil treatments was measured for physiological performance, water use efficiency and yield. Mulch used on structurally fragile tropical soil enriched with calcium was found to delay increased penetration resistance from hardening by wet/dry cycles. In this context, an improved soil rootability led to an enlargement of the leaf area index, greater nitrogen uptake and increased CO2 assimilation. This had important physiological consequences due to the positive effect on increased dry matter production and maize yield. In addition, these results suggested that mulch, used with urea, can delay the water supply for 3 or 4 days due to improvements in soil rootability caused by calcium and organic matter interactions. This may be crucial to a region where small intervals without rain are increasingly common due to global climate change. Therefore, due to a greater water use efficiency, this strategy may be a profitable way to increase crop productivity in tropical conditions rather than increasing water and nutrient application alone.

Understanding the effects of crop management practices on weed survival and seed production is imperative in improving long-term weed management strategies, especially for herbicide-resistant weed populations. Kochia [Bassia scoparia (L.) A.J. Scott] is an economically important weed in western North American cropping systems for many reasons, including prolific seed production and evolved resistance to numerous herbicide sites of action. Field studies were conducted in 2014 in a total of four field sites in Wyoming, Montana, and Nebraska to quantify the impact of different crop canopies and herbicide applications on B. scoparia density and seed production. Crops used in this study were spring wheat (Triticum aestivum L.), dry bean (Phaseolus vulgaris L.), sugar beet (Beta vulgaris L.), and corn (Zea mays L.). Herbicide treatments included either acetolactate synthase (ALS) inhibitors effective on non-resistant B. scoparia or a non–ALS inhibiting herbicide effective for both ALS-resistant and ALS-susceptible B. scoparia. Bassia scoparia density midseason was affected more by herbicide choice than by crop canopy, whereas B. scoparia seed production per plant was affected more by crop canopy compared with herbicide treatment. Our results suggest that crop canopy and herbicide treatments were both influential on B. scoparia seed production per unit area, which is likely a key indicator of long-term management success for this annual weed species. The lowest germinable seed production per unit area was observed in spring wheat treated with non–ALS inhibiting herbicides, and the greatest germinable seed production was observed in sugar beet treated with ALS-inhibiting herbicides. The combined effects of crop canopy and herbicide treatment can minimize B. scoparia establishment and seed production.

Tolpyralate is a new Group 27 pyrazolone herbicide that inhibits the 4-hydroxyphenyl-pyruvate dioxygenase enzyme. In a study of the biologically effective dose of tolpyralate from 2015 to 2017 in Ontario, Canada, tolpyralate exhibited efficacy on a broader range of species when co-applied with atrazine; however, there is limited published information on the efficacy of tolpyralate and tolpyralate+atrazine relative to mesotrione and topramezone, applied POST with atrazine at label rates, for control of annual grass and broadleaf weeds. In this study, tolpyralate applied alone at 30 g ai ha−1 provided >90% control of common lambsquarters, velvetleaf, common ragweed, Powell amaranth/redroot pigweed, and green foxtail at 8 weeks after application (WAA). Addition of atrazine was required to achieve >90% control of wild mustard, ladysthumb, and barnyardgrass at 8 WAA. Tolpyralate+atrazine (30+1,000 g ai ha−1) and topramezone+atrazine (12.5+500 g ai ha−1) provided similar control at 8 WAA of the eight weed species in this study; however, tolpyralate+atrazine provided >90% control of green foxtail by 1 WAA. Tolpyralate+atrazine provided 18, 68, and 67 percentage points better control of common ragweed, green foxtail, and barnyardgrass, respectively, than mesotrione+atrazine (100+280 g ai ha−1) at 8 WAA. Overall, tolpyralate+atrazine applied POST provided equivalent or improved control of annual grass and broadleaf weeds compared with mesotrione+atrazine and topramezone+atrazine.

Systemic uptake of organic compounds from roots to leaves follows a Gaussian distribution in relation to the lipophilicity, as measured by the log Kow. Quantification of compound uptake with different lipophilicities, and applied as a seed treatment that diffuses through the seed coat into the embryo during imbibition, has not been reported. The aim of this investigation was to quantify the uptake of non-ionic compounds into seeds of soybean and corn. A series of fluorescent piperonyl amides were synthesized and a novel combinatorial pharmacodynamic technique was developed that provided a range of compounds from log Kow 0.02 to 5.7. Seeds were treated with a mixture of amides, imbibed and compounds chemically extracted and quantified by high-performance liquid chromatography using a fluorescence detector. The maximum uptake efficiency of the applied amide mixture from whole soybean and corn seeds was 67% at log Kow 2.9, and 43% at log Kow 3.4, respectively. The critical partition coefficient for uptake for both species was <4.2 log Kow. Seeds were dissected and separated as soybean embryo and testa, and corn internal tissues (embryo + endosperm) or seed covering layers (pericarp + testa), and >75% of the amides were found in the soybean embryo or corn internal tissues compared with the covering layers at log Kow <4.2. The distribution of amides showed that the corn seed covering layer had similar hydrophilic/lipophilic properties as internal tissues, while soybean tissues had different hydrophilic/lipophilic properties. Collectively, the Gaussian uptake pattern for systemic uptake into plants was not found for either seed species.

WeedSOFT is a state-of-the-art decision support system for weed management in the north central region of the United States, but its accuracy to predict corn yield loss associated with later-emerging weed communities has not been adequately assessed. We conducted experiments in 2004 and 2005 to compare observed and predicted corn yield related to four establishment times of mixed-species weed communities for validation of competitive index modifier (CIM) values in WeedSOFT. Weed communities were established at VE, V2, V4, and V6 corn (emergence, second-leaf, fourth-leaf, and sixth-leaf stages, respectively), and consisted largely of annual grass and moderately competitive annual broadleaf species. Compared to weed-free corn, yield loss occurred in each of seven site-years for weed communities established at VE corn, but in only one site-year for communities established at V2 corn. No corn yield loss was associated with weed communities established at V4 or V6 corn. For communities established at VE corn, predicted corn yield differed from observed yield in all but one site year, with predicted yield less than observed yield in three site-years, and greater than observed yield in two site-years; however, nonlinear regression analyses of yield data pooled over site-years showed that fitted values were similar between predicted and observed yield. For communities established at V2 and V4 corn, predicted yield was less than observed yield in six and five site-years, respectively. For communities established at V6 corn, predicted yield was less than observed yield in three of six site-years, but was similar to observed yield in three of six site-years. These results indicated that the CIM values in WeedSOFT tended to overestimate the competitiveness of late-emerging weed communities. Corn yield data from a pooled analysis of all site-years were used to generate a revised set of growth stage CIM values, which improved the accuracy of predicted corn yield. These results should improve weed management decisions and reduce the need for herbicide applications to late-emerging weeds.

Striga is a major constraint to food production in Africa. Most technologies developed for the eradication of Striga asiatica from the United States are not adaptable to Africa. Imazapyr and pyrithiobac coated imidazolinone-resistant (IR)-resistant maize seed prior to planting at rates of 30 to 45 g ha−1 provide near season long control of Striga and can increase maize yields three- to fourfold if supplied with fertilizer. Slow release seed coatings reduce maize injury when post-planting rains are sparse and improve Striga control when there is excessive rainfall early in the season. Models suggest that herbicide resistance may not be a significant threat in short season maize, but vigilance in removing flowering Striga plants that are not controlled is recommended due to the known risk of evolution of resistance to these herbicides. Stacking the IR gene with glyphosate resistance and using imazapyr treated seed and applying glyphosate mid-season would provide season long Striga control and delay the evolution of resistance to both herbicides. To date, adoption of this technology has been limited by a number of factors. However, it should be included as one component of a multi-factor approach to increasing maize productivity in areas of Africa where Striga is problematic.

Nearly all commercial sweet corn fields contain weeds that escaped management and, therefore, sweet corn often suffers yield losses due to weed competition. For this reason, field trials were conducted from 2009 to 2011 near Prosser, WA and Urbana, IL to evaluate the responses of weeds and four sweet corn hybrids to three levels of weed management; weed free, high intensity cultivation (HC), and low intensity cultivation (LC). Weed management level had the greatest impact on early season weed densities and HC reduced final weed biomass more than LC in 2 of 4 site-years. Two taller sweet corn hybrids with greater leaf area suppressed final weed biomass more than two shorter hybrids with less leaf area in 3 of 4 site-years. When grown with less intense weed management that resulted in more weeds, taller sweet corn hybrids with greater leaf area maintained yields better than shorter, less competitive sweet corn hybrids. Utilizing hybrids with greater tolerance to weeds and greater ability to suppress weeds could be a valuable component of an integrated weed management system.

Regional distributions of crop diversity are important to take into account for the spatial design of in situ, farmer-participatory interventions in crop genetic management. Regional seed flows are an important factor in shaping geographical distributions of crop diversity. This study contributes to the insight in these seed flows, focusing on maize (Zea mays L.) in Chimaltenango, an area in the western highlands of Guatemala. A social survey of 257 households on different aspects of seed management produced information on cultivar naming, seed sources, reasons and causes of the discontinuation of seed lots, and important explanatory variables associated with different seed sources. A small portion of the reported seed lots originated from regional seed sources. The main motivation of regional seed exchange and the discontinuation of seed lots was to achieve change in plant characteristics of the crop, especially to obtain lower plants and shorter growing cycles. It is argued that farmer selection fails to achieve such change, and in fact leads to an equilibrium with high plants and long growing cycles. Seed exchange functions as an escape to this trend. Other factors of influence on seed exchange are altitude and ethnicity. The study also highlights the issue of geographical directionality in seed exchange patterns.

The aim of this study was to quantify the long-term effects of Al in solution on mineral ion uptake and associated H+ release by the roots of maize cultivated in conditions compatible with field data. Two maize (Zea mays L.) cvs of differing sensitivity to Al were compared. The culture was conducted in hydroponic conditions for culture periods of 2–5 d in the presence of 0–30 μM Al. Proton fluxes were localized along the roots using a videodensitometry method with bromocresol green on agarose gel.

Aluminium reduced the uptake of anions, particularly NO3−, whereas the uptake of K+ and NH4+ was unaffected. These effects were accompanied by an increase in net H+ release which was quantitatively comparable with the reduction in NO3− uptake. The reduction in NO3− uptake and the concomitant increase in H+ release increased with the quantity of Al accumulated in the roots, which was itself dependent on the concentration of Al in solution. The Al-sensitive cultivar bound more Al to its roots than the Al-tolerant one; the reduction in NO3− uptake and the increase in H+ release were also greater in the Al-sensitive cv. Dye indicator videodensitometry showed that the increased H+ release was general all along the roots.

These results led us to specify the relation between the Al deposition in roots, the reduction in NO3− uptake and the stimulation in rhizosphere acidification by the plants. They show that, with maize, Al induces an inhibition in NO3− uptake and an increase in net H+ release in a 1[ratio ]1 ratio. These concomitant effects suggest that inhibition in NO3−/OH− exchange unmasks active H+ excretion which is otherwise insensitive to Al. Both these effects are closely related to the Al content in roots expressed per unit root length.