The effects of low and high frequency ultrasound on the production of volatile compounds along with their derivation and corresponding off-flavours in milk and milk products are discussed in this review. The review will simultaneously discuss possible mechanisms of applied ultrasound and their respective chemical and physical effects on milk components in relation to the production of volatile compounds. Ultrasound offers potential benefits in dairy applications over conventional heat treatment processes. Physical effects enhance the positive alteration of the physicochemical properties of milk proteins and fat. However, chemical effects propagated by free radical generation cause redox oxidations which in turn produce undesirable volatile compounds such as aldehydes, ketones, acids, esters, alcohols and sulphur, producing off-flavours. The extent of volatile compounds produced depends on ultrasonic processing conditions such as sonication time, temperature and frequency. Low frequency ultrasound limits free radical formation and results in few volatile compounds, while high ultrasonic frequency induces greater level of free radical formation. Furthermore, the compositional variations in terms of milk proteins and fat within the milk systems influence the production of volatile compounds. These factors could be controlled and optimized to reduce the production of undesirable volatiles, eliminate off-flavours, and promote the application of ultrasound technology in the dairy field.

Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) is recognized as an invasive pest in Europe and North America. In Mexico, it is one of the main insect pests of soft-skinned fruits such as blueberries, strawberries, raspberries, blackberries, plums, and guava. Previous studies have shown that D. suzukii uses visual and chemical cues during host plant searching. This knowledge has been used to develop traps and attractants for monitoring D. suzukii. In this study, five trap designs were evaluated to monitor D. suzukii under field conditions. Traps were baited with SuzukiiTrap®, Z-Kinol, an attractant based on acetoin and methionol, or apple cider vinegar (ACV) enriched with 10% ethanol (EtOH) with the synergistic action of carbon dioxide (CO2). Our results suggested that the attractant was the determining factor in capturing D. suzukii, while trap design seemed to play a modest role. We found that traps baited with Z-Kinol captured the highest number of D. suzukii compared to that caught by traps baited with SuzukiiTrap®, or ACV + EtOH + CO2. The highest catch numbers occurred in blackberry, followed by strawberry, raspberry, and blueberry. Traps captured more females than males. The results obtained may be useful for monitoring D. suzukii populations in Mexico and elsewhere, particularly in states where soft fruit crops are a component of agricultural activities.

The poultry red mite (PRM) is an obligatory haematophagous pest that causes substantial economic losses in poultry worldwide. The PRM does not live on the host but in the bird's environment and must find its host remotely. Hence, manipulating chicken odours is of interest. Several crude plant-originating volatile organic compounds (VOCs) have already been shown as repellent to Dermanyssus gallinae. We aimed to test whether these VOCs can interfere with PRM host-seeking behaviour by their oral administration to the poultry. The objectives were to determine (1) if hen odours are modified by supplemented feed ingestion and (2) if such treatment makes hens less attractive to the PRM. Chemical characterization by gas chromatography–mass spectrometry of the hen odour was conducted before and after the hens ingested the supplemented feed. The chromatograms obtained show that hen odour was substantially modified after the hens consumed it. Among the molecules recurrently detected from the supplemented hens, 26% were nearly absent in the unsupplemented hens. Behavioural choice tests to compare the effect of the modified and unmodified-host odours on the PRM show that some of the plant-originating emitted VOCs and the modified whole-hen odours were repellent to the PRM.

The Kempen system is a dairy feeding system in which diet is provided in the form of a compound feed (CF) and hay offered ad libitum. Ad libitum access to CF and hay allows cows in this system to achieve a high DM intake (DMI). Out of physiological concerns, the voluntary hay intake could be increased and the consumption pattern of CF could be manipulated to maintain proper rumen functioning and health. This study investigated the effects of an artificial hay aroma and CF formulation on feed intake pattern, rumen function and milk production in mid- to late-lactating dairy cows. Twenty Holstein–Friesian cows were assigned to four treatments in a 4 × 4 Latin square design. Diet consisted of CF and grass hay (GH), fed separately, and both offered ad libitum, although CF supply was restricted in maximum meal size and speed of supply by an electronic system. Treatments were the combination of two CF formulations – high in starch (CHS) and fibre (CHF); and two GH – untreated (UGH) and the same hay treated with an artificial aroma (TGH). Meal criteria were determined using three-population Gaussian–Gaussian–Weibull density functions. No GH × CF interaction effects on feed intake pattern characteristics were found. Total DMI and CF intake, but not GH intake, were greater (P < 0.01) in TGH treatment, and feed intake was not affected by type of CF. Total visits to feeders per day, visits to the GH feeder, visits to the CF feeder and CF eating time (all P < 0.01) were significantly greater in cows fed with TGH. Meal frequency, meal size and meal duration were unaffected by treatments. Cows fed CHF had a greater milk fat (P = 0.02), milk urea content (P < 0.01) and a greater milk fat yield (P < 0.01). Cows fed TGH had a greater milk lactose content and lactose yield (P < 0.05), and milk urea content (P < 0.01). Cows fed TGH had smaller molar proportions of acetic acid and greater molar proportions of propionic acid compared with UGH. In conclusion, treatment of GH with an artificial aroma increased CF intake and total DMI, but did not affect hay intake. Additionally, GH treatment increased the frequency of visits to both feeders, and affected rumen volatile fatty acid profile. Type of CF did not affect meal patterns, ruminal pH, nor fermentation profiles.

The spontaneous fermentation of butter produces a highly fragrant product called smen. The short chain fatty acids (C4 to C10) have been traditionally considered the main aromas of this product but our work on the volatile fraction characterisation using SPME GC/MS showed that other products may also be important. Indeed, 183 different volatile compounds were detected belonging mainly to carboxylic acids, esters, ketones, nitrogen compounds and terpenes. However, only 19 compounds were common to more than 50% of the samples. These include short chain fatty acids (C4 to C10), their esters and short chain methylketones (C5 to C11). Despite their high levels in all samples, the amounts of C4 to C10 free fatty acids could not explain the differences in preparation and consumer appreciation of the samples. In contrast, the levels and nature of some methylketones and esters, particularly butanoic and hexanoic ethyl esters and 2-pentanone, seem to better reflect the effects of salt, thyme, acidity and consumer preferences.

Landraces are an important resource for crop breeding, due to their resilience and content of quality traits. However, genetic and phenotypic variability needs to be carefully characterized for proper direct and indirect use. In the present study, a multidisciplinary approach was carried out to assess the Italian sweet pepper landrace ‘Friariello’. A total of 18 traditional accessions were compared with five hybrids and two ecotypes with similar fruit typology. Genetic and morpho-agronomic characterization allowed us to distinguish five different group types of ‘Friariello’. Accessions showing two/three lobes at the blossom end of the fruit were found to be the most productive, whereas the genotypes showing one/two lobes at the blossom end were the most homogeneous. A total of 167 volatile organic compounds (VOCs) were identified in the collection analysed. Moreover, of the 37 targeted VOCs, 29 showed significant differences in content among the pepper genotypes studied. Of such VOCs related to main flavours described for pepper in the literature, ten were found to be the major determinants of variability among the derived ‘Friariello’ groups. A slightly negative, albeit not significant, correlation was observed between ascorbic acid (AsA) content and agronomic traits, suggesting a better quality for less productive accessions, but also the possibility to improve yield without significantly reducing the AsA levels. The approach used allowed us to define how the different typologies can be used for different breeding purposes, integrating the peculiar properties in order to establish a desirable landrace ideotype. Furthermore, valuable sources for improving quality traits in pepper breeding were identified.

In imbibing seeds, resumption of metabolism leads to the unavoidable release of volatile by-products that are perceived as cues by rodent seed predators. The crypsis hypothesis proposes that the primary function of a water-impermeable, hard seed coat is to reduce rodent seed predation by rendering seeds olfactorily cryptic. In an opinion paper, Jayasuriya et al. (2015) find the crypsis hypothesis unscientific and ‘not consistent with Darwin's theory of evolution by natural selection’. It is unfortunate that Jayasuriya et al. (2015) did not appreciate that the crypsis hypothesis offers an alternative explanation for the evolution of water-impermeable seeds: released seed volatiles are cues used by rodents to locate seeds, and variation in seed-coat permeability leading to differences in seed volatile release represents the variable under selection. Furthermore, the sealing of water-impermeable seed coats imposes a cost of increased generation time and, therefore, dormancy-release mechanisms are expected to subsequently evolve in response to local environmental conditions. We also disagree with most other claims by Jayasuriya et al. (2015), who failed to appreciate how species with dimorphic seeds – one morph with permeable and the other with impermeable seed coats – benefit from rodent caching behaviour and population dynamics. We welcome this opportunity to clarify and elaborate on key features and the evolution of water-impermeable seed coats according to the crypsis hypothesis.

Introduction. Detarium senegalense J.F. Gmel is a forest tree found in Senegal whose fruits are locally called ditax in Wolof. It is eaten fresh but it is widely used as nectar, which is one of the most popular beverages in Senegal. However, the chemical characterization of ditax pulp remains incomplete. This paper describes the volatile compounds of ditax to assess its organoleptic qualities. Materials and methods. Free volatile compounds of fresh ditax pulp were isolated by solvent-assisted flavor evaporation and analysis by GC-MS. Results and discussion. Among the 53 compounds tentatively identified, 49 are reported for the first time in this fruit. In total, 17 aldehydes, 11 aliphatic alcohols, 1 terpene alcohol, 7 free fatty acids, 3 unsaturated hydrocarbons, 1 terpene hydrocarbon, 7 sesquiterpene hydrocarbons, 1 phenol, 2 ketones, 2 esters and 1 organic acid compound were tentatively identified in ditax fresh pulp. The main volatiles identified in fresh ditax pulp were trans, cis-2,6-nonadienal (2.47 mg×kg–1), cis-2-heptenal (1.93 mg×kg–1), trans-a-bergamotene (1.11 mg×kg–1), bicyclo [2,2,0] hexane-1-carboxaldehyde (0.80 mg×kg–1), butyl octadecanoate (0.55 mg×kg–1) and trans-2-nonenal (0.47 mg×kg–1 fresh pulp). Conclusion. Among the volatile compounds identified, aldehyde compounds were widely predominant. To assess the aromatic qualities of ditax pulp, the primary impact aromas should be determined by identifying the aroma-active compounds by GC-olfactometry.

Introduction. Wines produced in cool climate regions may be competitive with wines obtained in traditional wine-producing countries. The aim of this paper was to conduct a quality analysis of the chemical composition of selected varieties of cool-climate grapes and to assess the enological parameters of wines obtained from them. Materials and methods. The chemical composition of 11 varieties of grapes as well as the basic enological parameters, profile of volatile components, and antioxidant and sensory properties of wines obtained from selected varieties were assessed. Results and discussion. The extract content of the assessed varieties of grapes varied within the range of 128.5–218.5 g×kg–1. The fruit was characterised by similar acidity but significantly heterogeneous antioxidant activity. The basic quality parameters of wines were in accordance with the EU regulations. Antioxidant activity and polyphenol content in red wines were approximately 5–7 times higher than those in white wines. In the sensory assessment the wines obtained high grades. Conclusion. Wines from the cool climate regions fulfil the EU normative requirements and are characterised by original sensory features. They may compete with products from traditional wine-producing countries.

Introduction. The aim of this study was to determine the ability of an electronic nose (e-nose) to predict the quality of nectarines and peaches, and, in particular, the aroma. Materials and methods. Four nectarine cultivars (‘María Dolce’, ‘Maillarlate’, ‘Nectaross’ and ‘Venus’) and one peach cultivar (‘Royal Glory’) were evaluated. The fruit was harvested ripe and the quality evaluations were carried out just one day after harvest. The intensity of the main descriptors of fruit quality was described, and fruits were subjected to an e-nose assessment. The sensory analysis and the e-nose results were presented through a Principal Component Analysis (PCA). A multiple linear regression (MLR) was also used to create a predictive model for the attribute ‘aroma’ compared with the other sensory parameters and the most informative e-nose sensor data. Results and discussion. ‘Royal Glory’ and ‘María Dolce’ were placed in a separate cluster far from ‘Venus’, ‘Nectaross’ and ‘Maillarlate’. The result of the MLR included the attributes ‘acidity’, ‘sweetness’ and ‘acceptability’ in the model, and the data registered by sensor 6 of the e-nose (SnO2-sensor, RGTO Mo, 45 Å thick layer), which were those factors that best related to the aroma, reached a R2 of 0.48 and a mean square error (MSE) of 3.85. It was concluded that the e-nose is an instrument able to discriminate peach varieties through their aromatic features, which are among the descriptors that mainly determine acceptability by the peach consumer.

Some of the seed-evolved volatiles, which were mainly composed of methanol, acetaldehyde, ethanol and acetone, caused the loss of seed germinability during storage. In general, the deleterious effects of the volatiles increased with increasing RH and temperature during storage. Acetaldehyde had the strongest deleterious effect regardless of RH and temperature, while ethanol caused seed deterioration only at high RHs. Acetone was slightly deleterious to some species, while methanol was almost inert in most seeds. Various aldehydes applied during storage showed some toxicity to seed germinability, which decreased, except for 3-methylbutanal, with increasing molecular size, suggesting that the endogenous volatile compounds with an aldehyde group cause seed deterioration. On the other hand, the contents of volatile compounds in seeds were higher when they were stored at 44% RH (water sorption zone 2), than when stored at 12% RH (water sorption zone 1). Acetaldehyde, the most deleterious volatile, was more abundantly accumulated within the seeds stored at −3.5°C than at 23°C. Based on these facts, it is suggested that endogenous volatiles, especially acetaldehyde, may be an important factor that accelerates seed deterioration which often occurs under lower RHs and/or temperatures throughout long-term storage.

Different raw beef quality traits from four local Spanish cattle breeds were studied using correlation, factorial, discriminant and multiple regression analysis. The following variables were studied after 0, 5, 10 and 15 days of storage under 60% O2, 30% CO2 and 10% N2 modified atmosphere packaging (MAP): colour physical variables, meat pigments, sensory degradation of odour and colour, microbial counts, thiobarbituric acid (TBA), pH, drip loss, lipid composition and volatile compounds. The degradation of raw beef quality was related to the increase in 2,3,3-trimethylpentane, 2,2,5-trimethylhexane, 3-methyl-2-heptene, 2-octene, 3-octene, 2-propanone, Enterobacteriaceae and aerobial plate counts (APC), metmyoglobin (MMb), lightness (L*), yellowness (b*), drip loss and TBA. Among these variables, TBA, b* and MMb may be useful in evaluating raw beef quality. No variables related to fat, except for TBA, including pH were limiting factors of the colour and odour shelf-life of raw beef under MAP. Each breed had some characteristics that were unique and these differences may influence the stability of meat to oxidation depending on myoglobin concentration and the polyunsaturated fatty acid (PUFA)/saturated fatty acid (SFA) ratio.

Lactococcus lactis strain AMP2I expresses OppA(D471R), a mutant oligopeptide binding OppA protein in which the aspartyl residue at position 471 was replaced by arginine. As a consequence of a different peptide transport in this strain, experimental Hispánico cheese made with Lc. lactis AMP2I had a higher content of total free amino acids than control cheese made with Lc. lactis AMP1I, an isogenic strain expressing wild-type OppA (Picon et al. 2005, 2007). In this work higher levels of diketones, hydroxy-ketones and, to a lesser extent, branched chain aldehydes were recorded for experimental cheese compared with control cheese. These differences levelled off as ripening proceeded. Strong correlations support the hypothesis that the increased levels of these volatile compounds in cheese made with Lc. lactis AMP2I are linked to higher concentrations of free amino acids threonine, valine and leucine.

Introduction. Aroma is often used as a fruit quality marker. Several researchers have used the analysis of volatile compounds to characterize dates (Phoenix dactylifera). Nevertheless, it is difficult to analyze this subtle aroma in spite of the many techniques available, such as liquid-liquid extractions and headspace analysis. The global approach of the e-nose with a minimum handling of the sample is a fast and efficient alternative to these techniques. This rather new tool, simulating the behavior of the human nose, thus aroused our interest for application to dates. Materials and methods. Dates were harvested, in 2005, at the Tamar stage from nine date varieties, in Zagora, Morocco. They were frozen after harvest and sent by air to the laboratory of CIRAD (Montpellier, France). All data were collected at the same time. Five hundred g of dates were taken at random, pitted and cut into small pieces. After homogenization, an aliquot of 3.0 g was put into a 10-mL headspace flask and immediately sealed. Six replicates per sample were prepared for later electronic-nose analysis. An electronic nose (e-nose) from Alpha MOS, equipped with Metal Oxide Sensors (MOS), was used. Data were analyzed using Principal Component Analysis (PCA). The Discrimination Index (DI), created by Alpha MOS, was used to describe the discriminatory power of the groups’ separation. Alpha MOS considers that good discrimination and reliability are achieved for DI over 80%. Results. Discrimination indexes > 88% and means of the coefficients of variation obtained for each sensor < 4% demonstrate the high performance and good reproducibility of the e-nose. Conclusion. The e-nose is sensitive to date volatile emissions. All the date varieties studied were well separated. This fast screening technique, creating a specific olfactory fingerprint as a genuine identity card, could be used to follow changes in maturity, assure the identity of lots of dates and detect adulteration.

The present study was designed to identify the volatile constituents across the oestrous cycle of bovine in order to detect oestrous-specific chemical signal. The bovine saliva was extracted with diethyl ether (1 : 1 ratio, v/v) and analysed by gas chromatography-linked mass spectrometry. Numerous compounds were identified during oestrous cycle of bovine saliva. Among these, the compounds, namely, trimethylamine, acetic acid, phenol 4-propyl, pentanoic acid and propionic acid were specific to oestrous stage. The behaviour assay revealed that the compound, trimethylamine, is involved in attracting the male animal. The result concludes that the trimethylamine is considered as a putative oestrous-specific salivary chemo-signal in the bovine.

Twenty-four low dry matter (DM) silages differing in fermentation quality were harvested at the same time from a crop that consisted mainly of timothy (Phleum pratense), and meadow fescue (Festuca pratensis). The silage samples were analysed by gas chromatography (GC) – mass spectrometry and gas chromatography – flame ionisation detection in order to determine and quantify volatiles present in silage. The voluntary intake of the 24 silages had been measured in a previous feeding trial with growing steers of Norwegian Red. Thirteen esters, five aldehydes, three alcohols, and one sulphide were identified and quantified. A total of 51 variables describing the chemical composition of the silages were included in a partial least-squares regression, and the relationship of silage fermentation quality to voluntary intake was elucidated. The importance of variables describing silage fermentation quality in relation to intake was judged from a best combination procedure, jack-knifing, and empirical correlations of the variables to intake. The GC-analysed compounds were mainly present in poorly fermented silages. However, compared with other explanatory chemical variables none of these compounds was of importance for the voluntary intake as evaluated by partial least-squares regression. A validated variance of 71% in silage DM intake was explained with the selected variables: total acids (TA), total volatile fatty acids (TVFA), lactic acid/total acid ratio and propionic acid. In this study extent (by the variable TA) and type of silage fermentation (by TVFA) influenced intake. Further, it is suggested that by restricting the fermentation in low DM grass silages the potential intake of silage DM is maximised.

Introduction. Chez Ananas comosus, les variations entre clones des caractéristiques organoleptiques sont faibles ; celles entre variétés sont plus marquées. Une dizaine d’hybrides Cayenne × Perolera a été sélectionnée par le Cirad pour être développée commercialement. Ils présentent des profils aromatiques distincts des parents. L’ananas doit être récolté relativement vert pour pouvoir parvenir en bon état sur les marchés consommateurs. L’arôme dégagé pouvant inciter le consommateur à acheter le fruit frais, nous avons étudié, sur différentes variétés et hybrides, l’impact du stade de maturation du fruit à la récolte et de la durée de conservation sur l’émission des composés volatils. Matériel et méthodes. Les différences de teneurs en composés volatils entre les variétés d’ananas ont pu être mesurées par extraction en espace de tête par SPME (fibre PDMS 100 µm), suivie d’une analyse CPG/FID sur colonne Stabilwax. Les analyses ont porté sur les fruits de quatre ou cinq clones de chacune des variétés Cayenne, Perolera, Queen, Rondon, ainsi que de cinq hybrides Cirad et d’un hybride MD2. L’impact du stade de maturité (extrait sec et acidité libre) et des conditions de conservation sur les composés volatils a été évalué. Résultats et discussion. Les profils aromatiques obtenus pour les clones d’une même variété ont été très semblables et les différences intervariétales se sont révélées répétables. Au cours de la maturation, une augmentation de l’émission des composés volatils s’est produite avant la pleine maturation. La conservation au froid entre 13 °C et 24 °C a provoqué une forte augmentation de l’émission aromatique. Conclusion. Différencier les profils aromatiques par SPME/GC-FID de différentes variétés d’ananas semble possible malgré leur grande similitude. Une identification plus poussée, par GC-MS, des composés volatils d’ananas de différentes origines pourrait permettre de mieux préciser leur caractérisation.

Introduction. L’identification des composés d’arôme des dattes permet d’apprécier leur qualité organoleptique ; elle revêt en outre un intérêt technologique en guidant les industriels dans certains processus de transformation du fruit et de production d’extraits d’arômes à partir des variétés de faible qualité, augmentant ainsi leur valeur marchande. Notre étude a eu pour objectif d’identifier des composés volatils responsables de l'arôme de huit variétés de dattes marocaines. Une classification statistique des variétés étudiées, basée sur leurs profils aromatiques, et une comparaison avec les résultats de travaux antérieurs effectués sur les composés d’arôme des dattes ont été également réalisées. Matériel et méthodes. Les composés volatils ont été analysés par la technique de piégeage dynamique de l’espace de tête et par désorption au micro-onde, puis ils ont été conjointement identifiés par chromatographie en phase gazeuse-spectrométrie de masse. L’étude statistique des données a été réalisée par analyse factorielle des correspondances et par classification hiérarchique ascendante. Résultats et discussion. Quarante-sept composés ont été identifiés dont vingt-trois non identifiés auparavant dans la datte. Cinq composés : la 2,3-pentanedione, le 2-méthyl-butanal, l’hexanal, le n-pentanol et le limonène se sont révélés être communs à toutes les variétés, tandis que certains composés ont été caractéristiques d’une variété particulière. La variété ‘Aziza’ s’est distinguée par la présence de cyclobutanol, de p-cymène et de 1,3-diméthyl-benzène. Le cyclopentadécadièn-1-one, l’éthanol et le géraniol ont été identifiés respectivement dans les variétés ‘Najda’, ‘Mejhoul’ et ‘Boufeggous’. En revanche, les variétés ‘Bouskri’ et ‘Iklane’ ont présenté une grande similarité de leurs profils aromatiques. Conclusion. Des différences de profils aromatiques entre les huit variétés étudiées ont été mises en évidence et des composés volatils « spécifiques » ont pu être identifiés.

In some European regions ewes' milk is transformed into cheese without a previous heat treatment. The microbiota and the native enzymes present in raw milk are considered to be responsible for the characteristic strong flavour of raw ewes' milk cheeses (Nuñez et al. 1989). Although pasteurization ensures a higher uniformity of the product, heat treatment of ewes' milk may impair the sensory characteristics of cheeses (Gaya et al. 1990).

Data on the pollination biology of neotropical aroids are still scarce and concern only a few species (Beath 1999, Croat 1997, Gibernau et al. 1999, 2000; Mayo et al. 1997 and references cited therein). It appears from these studies that Anthurium, Monstera and Spathiphyllum are on the whole pollinated mainly by bees (but see Kraemer & Schmitt 1999), whereas Dieffenbachia, Homalomena, Syngonium, Philodendron and Xanthosoma are generally beetle-pollinated. Although araceous inflorescences may be visited by several insect taxa (Madison 1979), only a few are the legitimate pollinators for each species (Seres & Ramirez 1995, Valerio 1984, Young 1986).