Fish assemblage structure of 29 Brittany streams is examined. Catchment area, stream gradient, and distance from sources are significantly correlated with species richness. Addition of species with increasing stream size occurs for all streams with no evident longitudinal replacement, suggesting little discontinuity in the stream geomorphology or temperature. Total density of fishes decreases significantly with an increase in stream size but no relation was found between total biomass and catchment area. Headwaters are characterized by short-lived, solitary, and nocturnal feeding species, whereas downstream sites are characterized by long-lived, schooling, diurnal feeding species. Presence of solitary and nocturnal feeding species upstream could be related to predation and/or competition factors. Temporal variation of individual species density varies from an upstream-downstream gradient with the highest variation upstream. Species responsible for this variation were mostly small benthic ones relatively difficult to sample, therefore, the greater temporal variation in upstream areas could be attributed to a deficiency of the sampling methodology.