A survey was conducted in the native tract of the Punjab Brown breed of chicken to study management practices, as well as morphological, performance and egg quality parameters. The study covered the three districts of Gurdaspur district in Punjab, and Ambala and Yamunanagar districts in Haryana, and included 532 birds and 61 families. Twenty-six microsatellite loci were used to assess genetic variability. The Punjab Brown is a multi-purpose breed, yielding good quality meat and eggs. Birds are reared in the backyard system and shelter is provided only during the night in the form of small enclosures mostly made up of mud and sometimes of wood. Average flock size is 8.7. Plumage colour is mostly brown and the pattern is usually solid but is sometimes spotted or striped. Males in particular have black spots/stripes on their neck, wings and tail. The comb is red, of single type and erect in position. The average weight of cocks and hens is 2.15±0.94 and 1.57±0.04 kg respectively. Hens start laying eggs at the age of about five to six months. Clutch size is about four to five. Average egg production is around 60–80 eggs per year. Eggshell colour is mostly light brown and average egg weight is 46.0±1.91g. The average weight of shell, albumin and yolk were 5.4±0.21, 24.4±0.63 and 16.2±0.48g respectively. Yolk index, albumin index and Haugh units were 0.41±0.005, 0.10±0.006 and 82.80±0.98 respectively. A total of 218 alleles were observed. The number of alleles per locus varied from 4–14. The mean PIC value for all the loci was 0.744. Twenty-four loci were found to be neutral (P<0.05) using Ewens Watterson test of neutrality. The exact test revealed that 15 loci deviated from Hardy Weinberg Equilibrium. The population has not undergone any recent bottleneck as revealed by quantitative and graphical qualitative tests.