Preliminary stable oxygen isotope data are presented from the southern North Sea Basin successions, ranging from the Lutetian to Rupelian. Analyses were performed on fish otoliths, nuculid bivalves and benthic foraminifera and are presented as bulk δ18O values relative to a well established regional sequence stratigraphic framework. The most significant positive shift in δ18O values clearly falls at the top of the regionally recognised Bassevelde 3 sequence, which base corresponds to the Eocene-Oligocene GSSP boundary. The here documented δ18O shift is closely associated with the base of the traditional Rupelian unit-stratotype and is tentatively correlated to the globally recognised Oi-1 event.