Establishing the timing of glacial and nonglacial intervals in the core regions of the Laurentide Ice Sheet (LIS) is essential to constrain ice-sheet configuration at times of globally reduced ice volume, such as during Marine Isotope Stage (MIS) 3 (~57–29 ka). Hudson Bay Lowland (HBL) deglaciation, at the centre of the LIS, has been inferred at MIS 3 based on near-infinite wood radiocarbon ages and limited luminescence ages. To better constrain the age of the penultimate deglaciation of the western HBL, this study initially identified the youngest intertill nonglacial sediments, based on extensive fieldwork and till characterization. Next, vetted radiocarbon ages were combined with revised stratigraphy to show that five previously identified “MIS 3” sites were likely deposited during an earlier ice-free period. Finally, new optical ages targeting the youngest intertill nonglacial bed at three localities yielded ages ranging from 166 to 146 ka; all older than MIS 3. These ages indicate that the penultimate deglaciation of the western HBL likely occurred during MIS 5e. This interpretation better explains accompanying paleobotanic data sets that indicate vegetation similar to vegetation existing under present interglacial conditions. Currently no firm evidence exists in the terrestrial stratigraphic record for the deglaciation of the western HBL during MIS 3.