A wide margin of crop safety is a desirable trait of POST herbicides, and investigation of crop tolerance is a key step in evaluation of new herbicides. Six field experiments were conducted in Ontario, Canada, from 2017 to 2018 to examine the influence of corn (Zea mays L.) hybrid (DKC42-60RIB, DKC43-47RIB, P0094AM, and P9840AM), application rate (1X and 2X), and application timing (PRE, V1, V3, and V5) on the tolerance of field corn to tolpyralate, a new 4-hydroxyphenyl pyruvate dioxygenase inhibitor, co-applied with atrazine. Two corn hybrids (DKC42-60RIB and DKC43-47RIB) exhibited slightly greater visible injury from tolpyralate + atrazine, applied POST, than P0094AM and P9840AM at 1 to 2 wk after application (WAA); hybrids responded similarly with respect to height, grain moisture, and yield. Applications of tolpyralate + atrazine at a 2X rate (80 + 2,000 g ai ha−1) induced greater injury (≤31.6%) than the field rate (40 + 1,000 g ha−1) (≤11.6%); the 2X rate applied at V1 or V3 decreased corn height and slightly increased grain moisture at harvest. On average, field rates resulted in marginally higher grain yields than 2X rates. Based on mixed-model multiple stepwise regression analysis, the air temperature at application, time of day, temperature range in the 24 h before application, and precipitation following application were useful predictor variables in estimating crop injury with tolpyralate + atrazine; however, additional environmental variables also affected crop injury. These results demonstrate the margin of corn tolerance with tolpyralate + atrazine, which provides a basis for optimization of application timing, rate, and corn hybrid selection to mitigate the risk of crop injury with this herbicide tank mixture.

Herbicide-resistant weeds are a growing concern globally; in response, new herbicide resistance traits are being inserted into crops. Isoxaflutole-resistant soybean [Glycine max (L.) Merr.] will provide a new mode of action for use in this crop. Ten experiments were conducted over a 2-yr period (2017, 2018) to determine herbicide interactions between isoxaflutole and metribuzin on soybean injury, weed control efficacy, and soybean yield on a range of soil types. Soybean leaf-bleaching injury caused by isoxaflutole was most severe at sites with higher levels of rainfall after application. Control of weed species with isoxaflutole (52.5, 79, and 105 g ai ha−1) and metribuzin (210, 315, and 420 g ai ha−1) differed by site based on amount of rainfall after application. At sites where there was sufficient rainfall for herbicide activation, isoxaflutole at all rates controlled common lambsquarters (Chenopodium album L.), Amaranthus spp., common ragweed (Ambrosia artemisiifolia L.), and velvetleaf (Abutilon theophrasti Medik.) >90%; metribuzin at all rates controlled Amaranthus spp. and witchgrass (Panicum capillare L.) >80%. Control of every weed species evaluated was reduced when there was limited rainfall after herbicide application. The co-application of isoxaflutole + metribuzin resulted in additive or synergistic interactions for the control of C. album, Amaranthus spp., A. artemisiifolia, A. theophrasti, Setaria spp., barnyardgrass [Echinochloa crus-galli (L.) P. Beauv], and P. capillare. Isoxaflutole and metribuzin can be an effective management strategy for common annual broadleaf and grass weeds in Ontario if timely rainfall events occur after herbicide application.

Effective POST herbicides and herbicide mixtures are key components of integrated weed management in corn; however, herbicides vary in their efficacy based on application timing. Six field experiments were conducted over 2 yr (2017–2018) in southwestern Ontario, Canada, to determine the effects of herbicide application timing and rate on the efficacy of tolpyralate, a new 4-hydroxyphenyl pyruvate dioxygenase inhibitor. Tolpyralate at 15, 30, or 40 g ai ha−1 in combination with atrazine at 500 or 1,000 g ai ha−1 was applied PRE, early POST, mid-POST, or late POST. Tolpyralate + atrazine at rates ≥30 + 1,000 g ha−1 provided equivalent control of common lambsquarters and Powell amaranth applied PRE or POST, whereas no rate applied PRE controlled common ragweed, velvetleaf, barnyardgrass, or green foxtail. Common ragweed, common lambsquarters, velvetleaf, and Powell amaranth were controlled equally regardless of POST timing. In contrast, control of barnyardgrass and green foxtail declined when herbicide application was delayed to the late-POST timing, irrespective of herbicide rate. Similarly, corn grain yield declined within each tolpyralate + atrazine rate when herbicide applications were delayed to late-POST timing. Overall, the results of this study indicate that several monocot and dicot weed species can be controlled with tolpyralate + atrazine with an early to mid-POST herbicide application timing, before weeds reach 30 cm in height, and Powell amaranth and common lambsquarters can also be controlled PRE. Additionally, this study provides further evidence highlighting the importance of effective, early-season weed control in corn.

Transgenic crops are being developed with herbicide resistance traits to expand innovative weed management solutions for crop producers. Soybean with traits that confer resistance to the hydroxyphenylpyruvate dioxygenase herbicide isoxaflutole is under development and will provide a novel herbicide mode of action for weed management in soybean. Ten field experiments were conducted over 2 years (2017 and 2018) on five soil textures with isoxaflutole-resistant soybean to evaluate annual weed control using one- and two-pass herbicide programs. The one-pass weed control programs included isoxaflutole plus metribuzin, applied PRE, at a low rate (52.5 + 210 g ai ha−1), medium rate (79 + 316 g ai ha−1), and high rate (105 + 420 g ai ha−1); and glyphosate applied early postemergence (EPOST) or late postemergence (LPOST). The two-pass weed control programs included isoxaflutole plus metribuzin, applied PRE, followed by glyphosate applied LPOST, and glyphosate applied EPOST followed by LPOST. At 4 weeks after the LPOST application, control of common lambsquarters, pigweed species, common ragweed, and velvetleaf was variable at 25% to 69%, 49% to 86%, and 71% to 95% at the low, medium, and high rates of isoxaflutole plus metribuzin, respectively. Isoxaflutole plus metribuzin at the low, medium, and high rates controlled grass species evaluated (i.e., barnyardgrass, foxtail, crabgrass, and witchgrass) 85% to 97%, 75% to 99%, and 86% to 100%, respectively. All two-pass weed management programs provided 98% to 100% control of all species. Weed control improved as the rate of isoxaflutole plus metribuzin increased. Two-pass programs provided excellent, full-season annual grass and broadleaf weed control in isoxaflutole-resistant soybean.

Field studies were conducted to determine watermelon tolerance and yield response when treated with bicyclopyrone preplant (PREPLANT), POST, and POST-directed (POST-DIR). Treatments consisted of two rates of bicyclopyrone (37.5 and 50 g ai ha–1), fomesafen (175 g ai ha–1), S-metolachlor (802 g ai ha–1), and a nontreated check. Preplant treatments were applied to formed beds 1 d prior to transplanting and included bicyclopyrone (37.5 and 50 g ha–1) and fomesafen (175 g ha–1), and new polyethylene mulch was subsequently laid above treated beds. POST and POST-DIR treatments were applied 14 ± 1 d after watermelon transplanting and included bicyclopyrone (37.5 and 50 g ha–1) POST and POST-DIR, and S-metolachlor (802 g ai ha–1) POST-DIR. POST-DIR treatments were applied to row middles, ensuring that no herbicide contacted watermelon vines or polyethylene mulch. At 2 wk after transplanting (WAT), 15% foliar bleaching was observed in watermelon treated with bicyclopyrone (50 g ha–1) PRE. At 3 WAT, bicyclopyrone (37.5 and 50 g ha–1) POST caused 16% and 17% foliar bleaching and 8% and 9% crop stunting, respectively. At 4 WAT, initial injury had subsided and bicyclopyrone (37.5 and 50 g ha–1) POST caused 4% and 4% foliar bleaching and 4% and 8% crop stunting, respectively. No symptoms of bleaching or stunting were observed at 6- and 8-WAT ratings. Watermelon total yield, marketable yield, total fruit number, marketable fruit number, and average fruit size were unaffected by herbicide treatments. Therefore, registration of bicyclopyrone (37.5 and 50 g ha–1) PREPLANT, POST, and POST-DIR would offer watermelon producers a safe herbicide option and a novel mode of action for weed management.

Common bermudagrass is a problematic weed within tall fescue turfgrass. Field research was conducted from 2010 to 2012 in Knoxville, TN, evaluating the efficacy of sequential applications of topramezone (12.5 and 25 g ha−1), triclopyr (1,120 g ha−1), and mixtures of topramezone + triclopyr for bermudagrass control in tall fescue turf. Sequential applications of fenoxaprop + triclopyr (100 + 1,120 g ha−1) were included for comparison. Three applications of each treatment were applied at 21-d intervals during July, August, and September of 2010 and 2011. Plots were stripped to receive tall fescue interseeding at 0 or 490 kg ha−1 during September 2010 and 2011. Bermudagrass control with topramezone + triclopyr mixtures was greater than topramezone or triclopyr applied alone 14 wk after initial treatment (WAIT) each year. In the second year of this study, topramezone + triclopyr mixtures controlled bermudagrass 27 to 50% compared to 27% for fenoxaprop + triclopyr by 52 WAIT. However, bermudagrass control with topramezone + triclopyr mixtures increased to 88 to 92% by 52 WAIT when accompanied with tall fescue interseeding at 490 kg ha−1. Future research should evaluate effects of interseeding on the efficacy of different herbicides for weed control in cool- and warm-season turf.

With rapid progress being made in deciphering plant genomic sequences, determining the function of these genes is one of the main challenges that plant molecular biologists face today. Herbicidal inhibitors have been very useful for understanding gene function in at least two examples, represented by herbicidal inhibitors of hydroxyphenylpyruvate dioxygenase (HPPD) and deoxyxylulosephosphate reductoisomerase (DXR). In the first, an albino mutant of Arabidopsis isolated during the study of carotenoid biosynthesis was found to have an intact carotenoid biosynthetic pathway. A number of “bleaching herbicides” in development at about the same time (e.g., sulcotrione) produced similar symptoms by strongly inhibiting HPPD, a key enzyme in plastoquinone biosynthesis. Examination of the Arabidopsis mutant revealed that the HPPD gene had been inactivated in the albino plants. Inhibition of the HPPD pathway also led to reduced levels of tocopherol (vitamin E), an end product of the pathway. Further studies and manipulation of the pathway produced plants with significantly higher levels of vitamin E. This result is a clear demonstration of how an herbicidal inhibitor was able to lead to the identification of a gene that was responsible for a particular phenotype. As a second example, identification of fosmidomycin as a specific inhibitor of DXR in the recently elucidated nonmevalonate pathway of isopentenyl pyrophosphate (IPP) biosynthesis was instrumental in furthering the understanding of an important route to synthesis of many important terpenoid products.

A waterhemp population (McLean County resistant, MCR) from McLean County, Illinois is resistant to both mesotrione and atrazine by elevated rates of herbicide metabolism. Research was conducted to investigate the inheritance of these resistance traits. Resistant and sensitive plants were crossed to obtain reciprocal F1 populations, which were then used to create pseudo-F2 and backcross (to sensitive parent; BCS) populations. The various populations were evaluated with whole-plant herbicide efficacy studies in a greenhouse. The responses of the F1 populations to both mesotrione and atrazine were intermediate when compared with parental populations. In the case of atrazine, BCS and F2 populations segregated 1 : 1 and 1 : 3, respectively, for susceptibility (S) : resistance (R), at a dose that controlled the sensitive parent but not the F1 or resistant parent. For mesotrione, variability was observed within the F1 populations, suggesting that mesotrione resistance is multigenic and the resistant parents used in the cross were not homozygous at the resistance loci. Furthermore, at low mesotrione doses, more F2 plants survived than expected on the basis of a single-gene trait, whereas at high doses, fewer F2 plants survived than expected. Dry weight data confirmed the conclusions obtained from survival data. Specifically, atrazine responses segregated into two discrete classes (R and S) in both the F2 and BCS populations, whereas mesotrione responses showed continuous distributions of phenotypes in F2 and BCS populations. We conclude that metabolism-based atrazine resistance in MCR is conferred by a single major gene, whereas inheritance of mesotrione resistance in this population is complex.

Field research was conducted in 2010 and 2011 to investigate the efficacy of herbicides for dallisgrass control when applied at various growing (GDD) or cooling degree day (CDD) –based application timings. Herbicide treatments included fluazifop-p-butyl (fluazifop; 105 g ai ha−1), mesotrione (280 g ai ha−1), tembotrione (92 g ai ha−1), topramezone (37 g ai ha−1), and tank mixtures of fluazifop plus mesotrione, tembotrione, or topramezone. Herbicide treatments were applied at either 75, 175, 375, 775 GDD, or 5 CDD. Treated plots were subjected to three tall fescue interseeding regimes: no seeding, seeding in spring, or seeding in fall (0, 353, and 353 kg pure live seed ha−1, respectively). In 2010, dallisgrass control from fluazifop applied at 75, 375, and 775 GDD was poor (< 50%) by 52 wk after treatment (WAT); in 2011, control from fluazifop application at these timings was higher (62 to 72%). When applied at 175 GDD or 5 CDD in 2010 and 2011, dallisgrass control from fluazifop ranged from 79 to 93% at 52 WAT. The addition of mesotrione, tembotrione, or topramezone to fluazifop did not affect dallisgrass control at any application timing, and control provided by these herbicides alone was low (< 65%). Interseeding tall fescue in the fall improved dallisgrass control from herbicides applied at 75 GDD in 2010 and 175, 375, and 775 GDD at 52 WAT in both years. Results suggest that timing of fluazifop applications at 175 GDD and 5 CDD enhances dallisgrass control.

The herbicides mesotrione and topramezone inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD) and have efficacy against smooth crabgrass. Research was conducted to determine the impacts of soil-applied nitrogen (N) fertilizer on the effectiveness of single applications of mesotrione and topramezone for postemergence smooth crabgrass control. Field experiments in 2010 and 2011 evaluated the efficacy of mesotrione (280 g a.i. ha−1) and topramezone (9 g a.i. ha−1) for control of multitiller smooth crabgrass subjected to five N fertility treatments (0, 12, 25, 37, or 49 kg N ha−1). Greenhouse experiments evaluated the response of smooth crabgrass to mesotrione (0, 70, 140, 280, 560, and 1,120 g ha−1) and topramezone (0, 4.5, 9, 18, 36, and 72 g ha−1) with 0 or 49 kg N ha−1. Further research evaluated changes in smooth crabgrass leaf tissue pigment concentrations following treatment with mesotrione (280 g ha−1) and topramezone (18 g ha−1) with 0 or 49 kg N ha−1. In field experiments, N increased smooth crabgrass control with mesotrione and topramezone for 8 wk; however, increasing N rate above 25 kg ha−1 did not improve control on any rating date. In dose-response experiments, N application reduced I50 values for mesotrione and topramezone by 67 and 53%, respectively, 21 d after treatment (DAT). Reductions in aboveground biomass with both herbicides were greater when applied following N treatment as well. In leaf-response experiments, N decreased new leaf chlorophyll and carotenoid concentrations and new leaf production after treatment with topramezone. Future research should investigate whether increased translocation of these herbicides to meristimatic regions contribute to N-enhanced efficacy.

Turfgrass managers can reduce foliar bleaching of smooth crabgrass from mesotrione by tank-mixing triclopyr ester with applications. These tank mixtures also have potential to enhance smooth crabgrass control compared to mesotrione alone. The objectives of this research were to evaluate the influence of triclopyr on the efficacy, absorption, and translocation of mesotrione in multitiller smooth crabgrass. In field experiments, tank-mixing triclopyr at 560 or 1,120 g ae ha−1 with mesotrione at 140 g ai ha−1 applied sequentially or at 280 g ha−1 applied singly provided excellent control (> 90%) of multitiller smooth crabgrass in tall fescue. These treatments were more effective than mesotrione alone and fenoxaprop at 195 g ai ha−1 that averaged 66 and 81% control after 6 wk, respectively. Mesotrione alone at 280 g ha−1 bleached smooth crabgrass 53% at 2 wk after initial treatment (WAIT), and was 14% greater than the 140 g ha−1 treatment. Sequential treatments of the low mesotrione rate bleached smooth crabgrass 16 to 22% from 3 to 5 WAIT. Triclopyr tank mixtures reduced smooth crabgrass bleaching from mesotrione on all dates to < 5%. Tall fescue injury was not detected at any evaluation date. In laboratory experiments, smooth crabgrass reached peak foliar absorption of 14C-mesotrione at 24 and 168 h after treatment (HAT; 23% and 15%) when mesotrione was applied with triclopyr at 1,120 and 0 g ha−1, respectively. Triclopyr reduced translocation of radioactivity 12% at 72 and 168 HAT, compared to 14C-mesotrione alone. Enhanced foliar uptake of mesotrione from triclopyr tank mixtures might improve control of multitiller smooth crabgrass compared to mesotrione alone. Reduced foliar bleaching from triclopyr is associated with less translocation of mesotrione or derivatives in smooth crabgrass.

Selective annual bluegrass (ABG) control with mesotrione is often inconsistent, and sequential applications might be required for complete control. The complementary nature of p-hydroxyphenylpyruvate dioxygenase (HPPD)- and photosystem II (PSII)-inhibiting herbicides is well documented. The HPPD-inhibiting herbicide mesotrione and the PSII-inhibiting herbicide amicarbazone both have efficacy against annual bluegrass and safety on certain cool-season turfgrasses. Topramezone is a HPPD-inhibiting herbicide being investigated for use in turfgrass. Field and greenhouse experiments were conducted to examine single applications of topramezone and mesotrione alone or in combination with amicarbazone for POST ABG control in spring. In greenhouse experiments, the combination of mesotrione (280 g ai ha−1) and amicarbazone (75 g ai ha−1) controlled ABG 70% by 21 d after treatment, > 29% more than either herbicide applied alone; these combinations were determined to be synergistic. Amicarbazone combined with topramezone (14.5 g ai ha−1) provided < 10% ABG control and was not synergistic. When combined with mesotrione, increasing amicarbazone rate to 150 or 255 g ha−1 did not increase ABG control compared to 75 g ha−1in field experiments. Combining mesotrione with amicarbazone resulted in a synergistic increase in POST ABG control at 1 and 2 wk after treatment (WAT). When applied alone or in combination with amicarbazone, increasing the mesotrione rate from 90 to 280 g ha−1 increased efficacy on ABG in field experiments. The combination of mesotrione at 280 g ha−1 and amicarbazone at 75 g ha−1 provided > 90% ABG control in field experiments. Future research should focus on sequential applications of mesotrione–amicarbazone combinations for ABG control in locations where ABG is historically more difficult to control.

We describe a 38-year-old woman who developed symptoms of hallucinogen persisting perception disorder (HPPD) after using lysergic acid diethylamide (LSD) once and continued to have symptoms of HPPD for nearly 17 years, although she did not use LSD after her first use. The symptoms of HPPD were temporally related to her first LSD use and she responded well to risperidone within a few weeks of commencing on the same. Although different medications have been tried in the management of HPPD including risperidone, olanzapine, clonidine, fluoxetine, sertraline, benzodiazepines and anti-epileptic medications, none have been proven to be the definitive medication of choice in the treatment of HPPD. Furthermore, there have been case reports suggesting worsening of symptoms with risperidone; however, the response to risperidone in our patient suggests the possibility of its effectiveness in the management of HPPD symptoms in some patients.