Field experiments were conducted in 2020 and 2021 to determine the effectiveness of electrocution on several weeds commonly encountered in Missouri soybean production using an implement known as The Weed Zapper™. In the first study, the effectiveness of electrocution on waterhemp, cocklebur, giant and common ragweed, horseweed, giant and yellow foxtail, and barnyardgrass was determined. Electrocution was applied when plants reached average heights and/or growth stages of 30 cm, 60 cm, flowering, pollination, and seed set. Electrocution was applied once or twice, at two different tractor speeds. Electrocution was more effective at the later plant growth stages. Pearson correlation coefficients indicated that control of weed species was most related to plant height and amount of plant moisture at the time of electrocution. When plants contained seed at the time of electrocution, viability was reduced from 54% to 80% among the species evaluated. A second study determined the effect of electrocution on late-season waterhemp plants, and also soybean injury and yield. Electrocution timings took place throughout reproductive soybean growth stages. The control of waterhemp escapes within the soybean trial ranged from 51% to 97%. Yield of soybean electrocuted at the R4 and R6 growth stages was similar to that of the nontreated control, but soybean yield was reduced by 11% to 26% following electrocution at all other timings. However, the visual injury and yield loss observed in these experiments likely represents a worst-case scenario because growers who maintain a clear height differential between waterhemp and the soybean canopy would not need to maintain contact with the soybean canopy. Overall, results from these experiments indicate that electrocution as part of an integrated weed-management program could eliminate late-season herbicide-resistant weed escapes in soybean, and reduce the number and viability of weed seed that return to the soil seedbank.

Tolpyralate is an herbicide that is usually mixed with atrazine for broad-spectrum weed control in corn. Previous research has provided information on the effective dose (ED) of tolpyralate applied alone and in a 1:33.3 mixture with atrazine; however, tolpyralate is commercially applied at a dose of 30 to 40 g ai ha−1 with a minimum of 560 g ai ha−1 of atrazine. Therefore, five field trials were conducted over 3 yr (2019 to 2021) to determine the ED of atrazine to complement 30 g ai ha−1 of tolpyralate to achieve 80%, 90%, and 95% control of seven weed species 2, 4, and 8 wk after application (WAA). Tolpyralate was applied alone and in a mixture with atrazine doses ranging from 50 to 2,000 g ai ha−1. At 8 WAA, the ED of atrazine for 95% control of velvetleaf, common ragweed, common lambsquarters, and wild mustard was below the minimum label dose of atrazine on the commercial tolpyralate label, ranging from 430 to 520 g ai ha−1, which supports the use of the minimum label dose of atrazine. In contrast, redroot pigweed required 1,231 g ai ha−1 of atrazine to complement tolpyralate for 95% control 8 WAA. At 8 WAA, barnyardgrass and a mixture of green foxtail and giant foxtail (Setaria spp.) were not controlled by 80%, 90%, or 95% with tolpyralate applied alone or co-applied with any dose of atrazine evaluated in this study. The results of this study conclude that tolpyralate + atrazine is highly efficacious on several weed species at atrazine doses of 40 to 130 g ai ha−1 below the label dose of 560 g ai ha−1, but the use of the higher dose of tolpyralate or another herbicide may be required to improve control of redroot pigweed and grass weed species.

Residual herbicides remain the primary tool for efficient weed control in cucurbit crops because of the lack of crop tolerance to many POST herbicide options. Field experiments were conducted in New Jersey in 2018 and 2019 to determine weed control efficacy and tolerance of direct-seeded cucumber ‘Python’ and summer squash ‘Gold Prize’ to S-metolachlor applied at 0.7 or 1.4 kg ai ha−1 at planting (PRE) or when crops reached the second- to third-leaf stage (EPOST). Regardless of applied rate, S-metolachlor PRE or EPOST provided 96% to 100% control 3 wk after planting (WAP) of smooth pigweed, large crabgrass, and giant foxtail. S-metolachlor PRE significantly improved American black nightshade and carpetweed control 3 WAP with respect to bensulide, and smooth pigweed with respect to clomazone + ethalfluralin. Summer squash showed excellent tolerance, regardless of S-metolachlor rate or timing of application, with stunting not exceeding 17% 4 WAP and 3% 7 WAP at the 1.4 kg ha−1 rate. Marketable yield decreased by 15% with S-metolachlor PRE or POST at 1.4 kg ha−1 with respect to clomazone + ethalfluralin, a reduction not noted when comparing with bensulide or the handweeded control. Marketable fruit number plant−1 and individual fruit weight were not affected by S-metolachlor applications. Conversely, cucumber was more sensitive to S-metolachlor than summer squash was with 30% seedling emergence reduction and 36% to 43% stunting 4 WAP when S-metolachlor was applied PRE at 1.4 kg ha−1. EPOST application resulted in 15% to 26% cucumber injury 1 wk after treatment. Marketable yield declined by 21% and 39% with the 0.7 and 1.4 kg ha−1 rates of S-metolachlor, respectively, compared with clomazone + ethalfluralin. Therefore, S-metolachlor may be a novel alternative to already labeled residual herbicides for summer squash, but unacceptable injury and yield reduction do not support its registration on cucumber.

PRE herbicides are the backbone of a successful weed management program in Christmas tree production. In a 2-yr field study, weed control efficacy and tolerance of newly transplanted Canaan fir to different PRE treatments were evaluated. Herbicide treatments consisted of two rates of each of atrazine plus mesotrione plus S-metolachlor at 561 + 150 + 1,504 and 1,122 + 300 + 3,008 g ai ha−1, flumioxazin at 214 and 429 g ai ha−1, hexazinone plus sulfometuron methyl at 289 + 27 and 480 + 46 g ai ha−1, indaziflam at 20 and 41 g ai ha−1, simazine plus oryzalin at 3,366 + 1,683 and 3,366 + 3,366 g ai ha−1, and a nontreated control. Averaged over 2 yr, all PRE treatments controlled giant foxtail, large crabgrass, and redroot pigweed at least 80% throughout the summer. Only the high rates of atrazine plus mesotrione plus S-metolachlor maintained >80% season-long control of yellow foxtail. Horseweed was controlled >85% with flumioxazin at both rates and at high rates of atrazine plus mesotrione plus S-metolachlor, hexazinone plus sulfometuron methyl, and indaziflam. The season-long PRE control of both red sorrel and wild carrot was maintained ≥80% with atrazine plus mesotrione plus S-metolachlor and hexazinone plus sulfometuron methyl regardless of application rate. By 16 wk after treatment, within-row densities of weeds evaluated in this study were reduced >75% in plots treated with atrazine plus mesotrione plus S-metolachlor at both application rates or hexazinone plus sulfometuron methyl at 480 + 46 g ai ha−1. Within-row weed densities in the nontreated control plots were 50, 32, 36, 25, 27, 31, and 19 plants m−2 for large crabgrass, giant foxtail, horseweed, redroot pigweed, red sorrel, wild carrot, and yellow foxtail, respectively. No discernible injury was observed in Canaan fir with any PRE treatment in both study years.

Transgenic crops are being developed with herbicide resistance traits to expand innovative weed management solutions for crop producers. Soybean with traits that confer resistance to the hydroxyphenylpyruvate dioxygenase herbicide isoxaflutole is under development and will provide a novel herbicide mode of action for weed management in soybean. Ten field experiments were conducted over 2 years (2017 and 2018) on five soil textures with isoxaflutole-resistant soybean to evaluate annual weed control using one- and two-pass herbicide programs. The one-pass weed control programs included isoxaflutole plus metribuzin, applied PRE, at a low rate (52.5 + 210 g ai ha−1), medium rate (79 + 316 g ai ha−1), and high rate (105 + 420 g ai ha−1); and glyphosate applied early postemergence (EPOST) or late postemergence (LPOST). The two-pass weed control programs included isoxaflutole plus metribuzin, applied PRE, followed by glyphosate applied LPOST, and glyphosate applied EPOST followed by LPOST. At 4 weeks after the LPOST application, control of common lambsquarters, pigweed species, common ragweed, and velvetleaf was variable at 25% to 69%, 49% to 86%, and 71% to 95% at the low, medium, and high rates of isoxaflutole plus metribuzin, respectively. Isoxaflutole plus metribuzin at the low, medium, and high rates controlled grass species evaluated (i.e., barnyardgrass, foxtail, crabgrass, and witchgrass) 85% to 97%, 75% to 99%, and 86% to 100%, respectively. All two-pass weed management programs provided 98% to 100% control of all species. Weed control improved as the rate of isoxaflutole plus metribuzin increased. Two-pass programs provided excellent, full-season annual grass and broadleaf weed control in isoxaflutole-resistant soybean.

Research was conducted from 2011 to 2015 to determine the effect of herbicide strategy on efficacy and evolution of herbicide resistance in weeds in a continuous glyphosate- and dicamba-resistant (GDr) soybean system. The nine herbicide strategies included sequential applications of glyphosate only, glyphosate plus dicamba with or without acetochlor, PRE application of residual herbicides with POST glyphosate or non-glyphosate herbicides, and their biennial rotation with one another. Giant foxtail and horseweed were the least problematic during all growing seasons. An increase in horseweed was observed by the end of the experiment especially in the plots where POST glyphosate was not used with PRE application of residual herbicides. Giant ragweed evolved resistance to glyphosate over a 4-yr period of selection with strategies that predominantly included PRE and POST glyphosate. Herbicide use strategies that included glyphosate-only and PRE application of residual herbicides fb POST glyphosate annually or in a biennial rotation were ineffective in controlling giant ragweed and glyphosate-resistant (GR) common waterhemp. Over the years, application of PRE herbicide mixtures before POST glyphosate application improved weed control and soybean yields compared with the glyphosate-only strategy. During all growing seasons, the greatest yield and reduction in total weed density before harvest was provided by herbicide use strategies that included glyphosate plus dicamba annually or in a biennial rotation regardless of the inclusion of acetochlor POST. Dicamba proved to be a valuable addition to improve the control of GR weeds. GDr soybean will provide growers with a new option for managing resistant weeds, but it needs to be used with caution, as multiple resistance in weeds, including waterhemp and giant ragweed, is already widespread.

Cover crop–based, organic rotational no-till (CCORNT) corn and soybean systems have been developed in the mid-Atlantic region to build soil health, increase management flexibility, and reduce labor. In this system, a roller-crimped cover crop mulch provides within-season weed suppression in no-till corn and soybean. A cropping system experiment was conducted in Pennsylvania, Maryland, and Delaware to test the cumulative effects of a multitactic weed management approach in a 3-yr hairy vetch/triticale–corn–cereal rye–soybean–winter wheat CCORNT rotation. Treatments included delayed planting dates (early, intermediate, late) and supplemental weed control using high-residue (HR) cultivation in no-till corn and soybean phases. In the no-till corn phase, HR cultivation decreased weed biomass relative to the uncultivated control by 58%, 23%, and 62% in Delaware, Maryland, and Pennsylvania, respectively. In the no-till soybean phase, HR cultivation decreased weed biomass relative to the uncultivated treatment planted in narrow rows (19 to 38 cm) by 20%, 41%, and 78% in Delaware, Maryland, and Pennsylvania, respectively. Common ragweed was more dominant in soybean (39% of total biomass) compared with corn (10% of total biomass), whereas giant foxtail and smooth pigweed were more dominant in corn, comprising 46% and 22% of total biomass, respectively. Common ragweed became less abundant as corn and soybean planting dates were delayed, whereas giant foxtail and smooth pigweed increased as a percentage of total biomass as planting dates were delayed. At the Pennsylvania location, inconsistent termination of cover crops with the roller-crimper resulted in volunteer cover crops in other phases of the rotation. Our results indicate that HR cultivation is necessary to achieve adequate weed control in CCORNT systems. Integration of winter grain or perennial forages into CCORNT systems will also be an important management tactic for truncating weed seedbank population increases.

Maximizing cereal rye biomass has been recommended for weed suppression in cover crop–based organic no-till planted soybean; however, achieving high biomass can be challenging, and thick mulch can interfere with soybean seed placement. An experiment was conducted from 2012 to 2014 in New York to test whether mixing barley and cereal rye would (1) increase weed suppression via enhanced shading prior to termination and (2) provide acceptable weed suppression at lower cover crop biomass levels compared with cereal rye alone. This experiment was also designed to assess high-residue cultivation as a supplemental weed management tool. Barley and cereal rye were seeded in a replacement series, and a split-block design with four replications was used with management treatments as main plots and cover crop seeding ratio treatments (barley:cereal rye, 0:100, 50:50, and 100:0) as subplots. Management treatments included high-residue cultivation and standard no-till management without high-residue cultivation. Despite wider leaves in barley, mixing the species did not increase shading, and cereal rye dominated cover crop biomass in the 50:50 mixtures in 2013 and 2014, representing 82 and 93% of the biomass, respectively. Across all treatments, average weed biomass (primarily common ragweed, giant foxtail, and yellow foxtail) in late summer ranged from 0.5 to 1.1 Mg ha−1 in 2013 and 0.6 to 1.3 Mg ha−1 in 2014, and weed biomass tended to decrease as the proportion of cereal rye, and thus total cover crop biomass, increased. However, soybean population also decreased by 29,100 plants ha−1 for every 1 Mg ha−1 increase in cover crop biomass in 2013 (P=0.05). There was no relationship between cover crop biomass and soybean population in 2014 (P=0.35). Soybean yield under no-till management averaged 2.9 Mg ha−1 in 2013 and 2.6 Mg ha−1 in 2014 and was not affected by cover crop ratio or management treatment. Partial correlation analyses demonstrated that shading from cover crops prior to termination explained more variation in weed biomass than cover crop biomass. Our results indicate that cover crop management practices that enhance shading at slightly lower cover crop biomass levels might reduce the challenges associated with excessive biomass production without sacrificing weed suppression in organic no-till planted soybean.

Switchgrass is a potential feedstock for cellulosic bioenergy production. Weed competition from annual grass during the establishment year can reduce switchgrass establishment and resulting productivity, but the relationship between early season grass densities and outcomes of competition are not well understood. We measured how a range of giant and yellow foxtail densities in the establishment year influenced switchgrass establishment and resulting productivity in the first production year (second year of the growing season). In two of the three site–yr more than four foxtail plants m−2 reduced switchgrass plant densities below documented thresholds of establishment success. A lesser effect of foxtails in the third site–year suggested that higher switchgrass emergence rates reduced foxtail competitive ability during establishment. Effects on yield were consistent over the three site–yr. The yield (10.96 Mg ha−1 ± 0.77) decreased rapidly as foxtail density increased. One foxtail plant m−2 reduced switchgrass yield in the first production year by 25%, and yield loss was 90% or greater at densities > 50 foxtail plants m−2. Although switchgrass can establish in the presence of foxtail competition, these weed species should be controlled to maximize yields in the first production year.

Integrated weed management (IWM) for agronomic and vegetable production systems utilizes all available options to effectively manage weeds. Late-season weed control measures are often needed to improve crop harvest and stop additions to the weed seed bank. Eliminating the production of viable weed seeds is one of the key IWM practices. The objective of this research was to determine how termination method and timing influence viable weed seed production of late-season weed infestations. Research was conducted in Delaware, Michigan, and New York over a 2-yr period. The weeds studied included: common lambsquarters, common ragweed, giant foxtail, jimsonweed, and velvetleaf. Three termination methods were imposed: cutting at the plant base (simulating hand hoeing), chopping (simulating mowing), and applying glyphosate. The three termination timings were flowering, immature seeds present, and mature seeds present. Following termination, plants were stored in the field in mesh bags until mid-Fall when seeds were counted and tested for viability. Termination timing influenced viable seed development; however, termination method did not. Common ragweed and giant foxtail produced viable seeds when terminated at the time of flowering. All species produced some viable seed when immature seeds were present at the time of termination. The time of viable seed formation varied based on species and site-year, ranging from plants terminated the day of flowering to 1,337 growing degree d after flowering (base 10, 0 to 57 calendar d). Viable seed production was reduced by 64 to 100% when common lambsquarters, giant foxtail, jimsonweed, and velvetleaf were terminated with immature seeds present, compared to when plants were terminated with some mature seeds present. Our results suggest that terminating common lambsquarters, common ragweed, and giant foxtail prior to flowering, and velvetleaf and jimsonweed less than 2 and 3 wk after flowering, respectively, greatly reduces weed seed bank inputs.

Eleven field experiments were conducted over a 3-yr period (2010, 2011, and 2012) in conventional- and no-till soybean with a flumioxazin and pyroxasulfone premix. PRE and preplant applications were evaluated for soybean injury, weed control, and yield compared to standard herbicides. Early-season soybean injury from flumioxazin/pyroxasulfone ranged from 1 to 19%; however, by harvest, soybean yields were similar across labeled rates (160 and 200 g ai ha−1), standard treatments, and the nontreated control. Flumioxazin/pyroxasulfone provided excellent control (99 to 100%) of velvetleaf, pigweed species (redroot pigweed and smooth pigweed), and common lambsquarters across almost all rates tested (80 to 480 g ai ha−1). Common ragweed, green foxtail, and giant foxtail control increased with flumioxazin/pyroxasulfone rate. The biologically effective rates varied between tillage systems. The flumioxazin/pyroxasulfone rate required to provide 80% control (R80) of pigweed was 3 and 273 g ai ha−1 under conventional- and no-till, respectively. For common ragweed, the R80 was 158 g ai ha−1 under conventional tillage; yet, under no-till, the rate was nonestimable. The results indicate that flumioxazin/pyroxasulfone can provide effective weed control as a setup for subsequent herbicide applications.

Effective weed seedbank management requires mechanistic understanding of ecological determinants of seed persistence in the soil seedbank. Chemical and physical defense of common lambsquarters, field pennycress, giant foxtail, kochia, velvetleaf, and yellow foxtail seeds were quantified in relation to short- and long-term seedbank persistence. Seed content of ortho-dihydroxyphenols (o-DHP), a class of putative seed defense compounds, varied more than threefold between the least protected species (common lambsquarters, 9.2 µg g seed−1) and the most protected species (kochia, 34.1 µg g seed−1). Seed o-DHP was inversely related (r = −0.77, P < 0.001) to seed half-life in the soil and to short-term seed persistence in burial assays (r = −0.82, P < 0.05). The relative importance of chemical seed protection in comparison to physical seed protection, as represented by the ratio of seed o-DHP concentration to seed coat thickness, decreased linearly with increasing short-term seed persistence (r = −0.96, P < 0.01) and nonlinearly with increasing long-term seed persistence in the soil seedbank (y = 0.16 + 0.21/(0.0432 + x), R2 = 0.99, P < 0.001). Mechanical damage to the seed coat, via piercing, slicing, or grinding treatments, increased short-term mortality during burial for all six species. Mortality of pierced seeds was negatively associated (r = −0.35, P < 0.05) with seed phenol concentration and positively associated with seed half-life (r = 0.42, P < 0.01) and seed coat thickness (r = 0.36, P < 0.05). Seed phenolics, as a class, supported the results for o-DHPs. Overall, these findings suggest a potential weakness, with respect to seedbank management, in the way weed seed defenses are constructed. Weed species with transient seedbanks appear to invest more in chemical defense than those species with highly persistent seedbanks. As a result, seeds in the latter category are relatively more dependent upon physical seed protection for persistence in the soil seedbank, and more vulnerable to management tactics that reduce the physical integrity of the weed seed coat.

Field experiments were conducted near Boone, IA, to quantify postdispersal seed predation of common lambsquarters and common waterhemp in corn (2003) and soybean (2004) managed with conventional, reduced, and zero-tillage systems. Seed predation in each tillage regime was quantified using selective exclusion treatments during July through September 2003 and June through October 2004. In addition, the activity density of ground-dwelling invertebrates was estimated with pitfall traps. Choice and no-choice feeding trials were conducted in the laboratory using the most abundant weed seed predators found in the field to determine seed preferences of the potential predator organisms. The greatest seed loss occurred during July and August. In 2003, seed predation was lower in zero tillage than in conventional and reduced tillages, but no differences in seed predation between tillage regimes were observed in 2004. Maximum seed predation for common lambsquarters was 53% in 2003 and 64% in 2004. Common waterhemp seed predation reached 80% in 2003 and 85% in 2004. The majority of seed predation was by invertebrate organisms. The most common invertebrate species captured with pitfall traps were field crickets (Gryllus pennsylvanicus De Geer [Gryllidae, Orthoptera]) and ground beetles (Harpalus pensylvanicus Burmeister [Coleoptera, Carabidae]). In 2003, field crickets were relatively more abundant in conventional and reduced tillage than in zero-tillage plots. In 2004, field crickets were more abundant in the reduced tillage than in the other two tillage regimes. No differences were detected for ground beetles among tillage regimes (P = 0.57). Choice and no-choice feeding experiments confirmed the preferences of field crickets and ground beetles for common lambsquarters and common waterhemp seeds over the larger seeds of giant foxtail and velvetleaf. Under field conditions, the activity density of field crickets was a significant predictor of common lambsquarters (r2 = 0.47) and common waterhemp (r2 = 0.53) seed predation. Positive relationships were also detected between the activity density of ground beetles and common lambsquarters (r2 = 0.30) and common waterhemp (r2 = 0.30) seed predation. This research demonstrated that weed seed predation is an important component affecting weed seedbanks and that crop management practices that favor the activity of predators such as field crickets or ground beetles could influence weed populations. Also, the results suggested that tillage is more important in determining the number of weed seeds available on the soil surface to predators than directly affecting predator activity density.

Synthetic seed banks are a useful tool for tracking how weed populations change over time. By sowing a known number of seeds of a given species within a quadrat with defined boundaries, an investigator can measure the number remaining and thereby calculate demographic rates (e.g., mortality). The alternative is to use in situ seeds and estimate their initial population density via sampling. To make a synthetic seed bank approach useful within an agricultural system subjected to soil disturbances such as tillage, one would need a way to account for seeds leaving the initial quadrat (i.e., a way to follow how the area encompassing the sown seeds changes over time). Without accounting for the change in location/extent of the synthetic seed bank, any field operation moving soil will create additional uncertainty in population size. Depending on the “aggressiveness” of specific field operations and the initial size of the quadrat, this effect might be negligible or so large as to be intractable. Here, we describe a method for following a synthetic seed bank over time using a “living boundary” of nondormant seeds, which effectively play the role of tracers used in the study of dynamics in other scientific disciplines. Study quadrats in East Lansing, MI, and Arlington, WI, were sown with giant foxtail and velvetleaf at a rate of 2,000 seeds m−2. The study quadrats were marked on the perimeter and diagonals using nondormant seeds of three marker species: kale, radish, and rye. The areas were then subjected to tillage and planting operations. Spatial coordinates of seedling locations for the marker and weed species were obtained through digital image processing. A nonparametric comparison of the spatial displacement of marker and weed species indicated that their empirical spatial distributions did not differ. The marker species quadrats described by the 50th, 90th, and 99th quantiles of movement contained all velvetleaf seedlings in Wisconsin, all velvetleaf seedlings in Michigan, and all giant foxtail seedlings in Michigan, respectively. The results suggest a simple rule for applying the method to field demography studies: after the original quadrat is deformed and seedlings have emerged, flag the polygon containing all marker seedlings to obtain the expanded quadrat containing the study weed population.