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Scanning Electron Microscopy of the Endometrium of Mares Infused with Gentamicin

Published online by Cambridge University Press:  17 March 2004

F.K. Al-Bagdadi ,
B.E. Eilts  and
G.F. Richardson
F.K. Al-Bagdadi
Affiliation:
Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
B.E. Eilts
Affiliation:
Department of Veterinary Clinical Science, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA
G.F. Richardson
Affiliation:
Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, P.E.I. C1A 4P3, Canada
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Abstract

Scanning electron microscopy (SEM) was used to study the endometrium of nine 1-year-old thoroughbred mares after twice intrauterine infusions of gentamicin, on 2 consecutive days. Five mares were infused on 2 consecutive days with 40 ml gentamicin (50 mg/ml) mixed with 80 ml of normal saline. Four mares served as controls and were infused with 120 ml of saline on 2 consecutive days. Endometrial biopsies were obtained from all mares 3 days after the second intrauterine infusion. Each biopsy was processed for SEM by standard methods. The endometrial epithelium of the gentamicin-infused mares had more cellular perforations than the saline-infused mares. The gentamicin-infused mares had less and shorter microvilli. The ciliated cells were fewer and some ciliated cells had disrupted and some had drooping cilia. The endometrial epithelium of the gentamicin-infused mares had a considerable number of endometrial cells that lost their luminal surfaces and some that lost their microvilli, compared to the saline-infused mares. We suggest that the information gathered in this pilot study should be used as basis for further investigation, on a larger scale basis, of the effects of repeated intrauterine infusion of gentamicin on the endometrial mucosa of mares.

Keywords

endometriumgentamicinmaresuterus
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 10 , Issue 2 , April 2004 , pp. 280 - 285
Copyright
© 2004 Microscopy Society of America

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