Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-02T22:56:02.415Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of Clitoria ternatea Leaf Extract on Growth and Morphogenesis of Aspergillus niger

Published online by Cambridge University Press:  03 July 2009

L. Kamilla ,
S.M. Mansor ,
S. Ramanathan  and
S. Sasidharan
L. Kamilla
Affiliation:
Centre for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
S.M. Mansor
Affiliation:
Centre for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
S. Ramanathan
Affiliation:
Centre for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
S. Sasidharan*
Affiliation:
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
*
Corresponding author. E-mail: [email protected]
Get access

Abstract

Clitoria ternatea is known for its antimicrobial activity but the antifungal effects of leaf extract on growth and morphogenesis of Aspergillus niger have not been observed. The extract showed a favorable antifungal activity against A. niger with a minimum inhibition concentration 0.8 mg/mL and minimum fungicidal concentration 1.6 mg/mL, respectively. The leaf extract exhibited considerable antifungal activity against filamentous fungi in a dose-dependent manner with 0.4 mg/mL IC50 value on hyphal growth of A. niger. The main changes observed under scanning electron microscopy after C. ternatea extract treatment were loss of cytoplasm in fungal hyphae and the hyphal wall and its diameter became markedly thinner, distorted, and resulted in cell wall disruption. In addition, conidiophore alterations were also observed when A. niger was treated with C. ternatea leaf extract.

Keywords

antifungal activityAspergillus nigerscanning electron microscopyClitoria ternatea
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 15 , Issue 4 , August 2009 , pp. 366 - 372
Copyright
Copyright © Microscopy Society of America 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bauer, R.W., Kirby, M.D.K., Sherris, J.C. & Turck, M. (1966). Antibiotic susceptibility testing by standard single disc diffusion method. Am J Clin Pathol 45, 493–496.Google Scholar
Bode, F.R., Pare, J.A.P. & Fraser, R.G. (1974). Pulmonary diseases in the compromised host. Medicine 53, 255–293.Google ScholarPubMed
Carlton, R.R., Deans, S.G., Gray, A.I. & Waterman, P.G. (1991). Antifungal activity of a flavonol glycoside from the leaves of bog myrtle (Myrica gale). Chemoecology 2, 69–71.CrossRefGoogle Scholar
Cos, P., Vlietinck, A.J., Berge, D.V. & Maes, L. (2006). Anti-infective potential of natural products: How to develop a stronger in vitro “proof-of-concept.” J Ethnopharmacol 106, 290–302.Google Scholar
Denning, D.W. (1998). Invasive aspergillosis. Clin Infect Dis 26, 781–805.Google Scholar
Galeotti, F., Barile, E., Curir, P., Dolci, M. & Lanzotti, V. (2008). Flavonoids from carnation (Dianthus caryophyllus) and their antifungal activity. Phytochem Lett 1, 44–48.Google Scholar
Gamliel, A., Katan, J. & Cohen, E. (1989). Toxicity of chloronitrobezenes to Fusarium oxysporum and Rhizoctonia solani as related to their structure. Phytoparas 17, 101–105.Google Scholar
Gomez, S.M. & Kalamani, A. (2003). Butterfly pea (Clitorea ternatea): A nutritive multipurpose forage legume for the tropics—An overview. Pakistan J Nutr 2, 374–379.Google Scholar
Ilić, S.B., Konstantinović, S.S. & Todorović, Z.B. (2004). Antimicrobial activity of bioactive component from flower of Linum capitatum kit. Facta Universitatis 3, 73–77.Google Scholar
Kontoyiannis, D.P. & Bodey, G.P. (2002). Invasive aspergillosis in 2002: An update. Eur J Clin Microbiol Infect Dis 21, 161–172.Google Scholar
Kontoyiannis, D.P., Mantadakis, E. & Samonis, G. (2003). Systemic mycoses in the immunocompromised host: An update in antifungal therapy. J Hosp Infect 53, 243–258.Google Scholar
Lim, Y.L., Kim, I.H. & Seo, J.J. (2007). In vitro activity of kaempferol isolated from the Impatiens balsamina alone and in combination with erythromycin or clindamycin against Propionibacterium acnes. J Microbiol 45, 473–477.Google ScholarPubMed
Londero, R.T. & Guadalupe-Cortés, J.M. (1990). Aspergiloses pulmonares. J Pneumol 16, 78–90.Google Scholar
Mukherjee, P.K., Kumar, V., Kumar, N.S. & Heinrich, M. (2008). The Ayurvedic medicine Clitoria ternatea—From traditional use to scientific assessment. J Ethnopharmacol 120, 291–301.CrossRefGoogle ScholarPubMed
Osborn, R.W., De Samblanx, G.W., Thevissen, K., Goderis, I., Torrekens, S., Van Leuven, F., Attenborough, S., Rees, S.B. & Broekaert, W.F. (1995). Isolation and characterization of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanceae and Saxifragaceae. FEBS Lett 368, 257–262.CrossRefGoogle ScholarPubMed
Pennington, J.E. (1980). Aspergillus lung disease. Med Clin North Am 64, 475–490.Google Scholar
Picman, A.K., Schneider, E.F. & Gershenzon, J. (1990). Antifungal activities of sunflower terpenoids. Biochem Syst Ecol 18, 325–328.Google Scholar
Sasidharan, S., Zuraini, Z., Yoga Latha, L. & Suryani, S. (2008). Fungicidal effect and oral acute toxicity of Psophocarpus tetragonolobus root extract. Pharmac Biol 46 261–265.Google Scholar
Schmourlo, G., Mendonc, R.R., Filho, A. & Alviano, C.S. (2005). Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants. J Ethnopharmacol 96, 563–568.Google Scholar
Stevens, D.A., Kan, V.L. & Judson, M.A. (2000). Practice guidelines for diseases caused by Aspergillus. Clin Infect Dis 30, 696–709.CrossRefGoogle ScholarPubMed
Young, R.C., Bennett, J.E., Vogel, C.L., Carbone, P.P. & DeVita, V.T. (1970). Aspergillosis. The spectrum of disease in 98 patients. Medicine 49, 147–173.Google Scholar