Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-26T19:08:59.408Z Has data issue: false hasContentIssue false
  • English
  • Français

Angioarchitecture of Tumors Induced by Two Different Cloned Cell Lines Established from a Transplantable Rat Malignant Fibrous Histiocytoma

Published online by Cambridge University Press:  21 November 2003

Ichiro Tsunenari ,
Jyoji Yamate ,
Masae Iwaki ,
Mitsuru Kuwamura ,
Takao Kotani  and
Sadashige Sakuma
Ichiro Tsunenari
Affiliation:
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehringer Ingelheim Co., Ltd., 3-10-1 Yato, Kawanishi, Hyogo 666-0193, Japan
Jyoji Yamate
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Masae Iwaki
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Mitsuru Kuwamura
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Takao Kotani
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Sadashige Sakuma
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Get access

Abstract

Angiogenesis, a biologic process whereby endothelial cells divide and migrate to form new blood vessels, is a key step in tumor growth, invasion, and metastasis. In the present study, we investigated the differences in angioarchitecture between two different tumors induced by cloned cell lines (MT-8 and MT-9), derived from a transplantable rat malignant fibrous histiocytoma, by scanning electron microscopy of vascular corrosion casts. During a 3-week observation period after implantation, the growth of MT-8 tumors appeared to be faster than that of MT-9 tumors. Histologically, MT-8 tumors were of the uniformly undifferentiated sarcoma type arranged in characteristic organoid structures, and MT-9 tumors showed a storiform growth pattern. In MT-8 tumors, neovascularization occurred by sprouting at postimplantation (PI) week 1, and the newly formed capillaries gradually became more tortuous. In MT-9 tumors, at PI week 1, the corrosion casts of newly formed capillaries mainly showed a wavy course but no fingerlike outgrowths of capillaries were seen. At PI weeks 2 and 3, the sprouting was seen specifically in MT-9 tumors, forming basketlike structures and glomeruloid structures of capillaries. These results indicate that angiogenesis or angioarchitecture of MT-8 tumors is different from that of MT-9 tumors, depending on the differences in their tumor histology and by the features like absence or presence of basketlike structures and glomeruloid structures of capillaries.

Keywords

angioarchitecturetumor angiogenesismalignant fibrous histiocytomatransplantationcorrosion castingrat
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 9 , Issue 6 , December 2003 , pp. 532 - 541
Copyright
© 2003 Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Arashiro, K., Ohtsuka, H., & Miki, Y. (1995). Three-dimensional architecture of human cutaneous vascular lesions: A scanning electron microscopic study of corrosion casts. Acta Derm Venereol (Stockh) 75, 257–263.Google Scholar
Auerbach, W. & Auerbach, R. (1994). Angiogenesis inhibition: A review. Pharm Ther 63, 265–311.Google Scholar
Enzinger, F.M. & Weiss, S.W. (1995). Malignant fibrohistiocytic tumors. In Soft Tissue Tumors, 3rd ed., Enzinger, F.M. & Weiss, S.W. (Eds.), pp. 351–380. St. Louis, MO: CV Mosby.
Ferrara, N. & Henzel, W.J. (1989). Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells. Biochem Biophys Res Commun 161, 851–858.CrossRefGoogle Scholar
Folkman, J. (1971). Tumor angiogenesis: Therapeutic implications. N Engl J Med 285, 1182–1186.Google Scholar
Folkman, J. (1992). The role of angiogenesis in tumor growth. Cancer Biol 3, 65–71.Google Scholar
Folkman, J. (1994). Angiogenesis and breast cancer. J Clin Oncol 12, 441–443.Google Scholar
Folkman, J. (1995). Angiogenesis in cancer, vascular, rheumatoid and other disease. Nature Med 1, 27–31.Google Scholar
Forsman, A.D. & McCormack, J.T. (1992). Microcorrosion casts of hamster luteal and follicular vasculature throughout the estrous cycle. Anat Rec 233, 515–520.Google Scholar
Graham, C.H., Rivers, J., Kerbel, R.S., Stankiewicz, K.S., & White, W.L. (1994). Extent of vascularization as a prognostic indicator in thin (<0.76 mm) malignant melanomas. Am J Pathol 145, 510–514.Google Scholar
Grunt, T.W., Lametschwandtner, A., Karrer, K., & Staindl, O. (1986a). The angioarchitecture of the Lewis lung carcinoma in laboratory mice (A light microscopic and scanning electron microscopic study). Scan Electron Microsc II, 557–573.Google Scholar
Grunt, T.W., Lametschwandtner, A., & Karrer, K. (1986b). The characteristic structural features of the blood vessels of the Lewis lung carcinoma (A light microscopic and scanning electron microscopic study). Scan Electron Microsc II, 575–589.Google Scholar
Kaidoh, T., Yasugi, T., & Uehara, Y. (1991). The microvasculature of the 7,12-dimethylbenz(a)anthracene(DMBA)-induced rat mammary tumour. 1. Vascular patterns as visualized by scanning electron microscopy of corrosion casts. Virchows Archiv A Pathol Anat 418, 111–117.Google Scholar
Kiso, Y., Yamashita, A., Sasaki, & F., Yamauchi S. (1990). Maternal blood vascular architecture of the dog placenta during the second half of pregnancy. Jpn Anim Reprod 36, 120–126.Google Scholar
Konerding, M.A., Miodonski, A.J., & Lametschwandtner, A. (1995). Microvascular corrosion casting in the study of tumor vascularity: A review. Scan Microsc 9, 1233–1244.Google Scholar
Macchiarelli, G., Nottola, S.A., Vizza, E., Familiari, G., Kikuta, A., Murakami, T., & Motta, P.M. (1993). Microvasculature of growing and atretic follicles in the rabbit ovary: A SEM study of corrosion casts. Arch Histol Cytol 56, 1–12.Google Scholar
Malkusch, W., Konerding, M.A., Klapthor, B., & Bruch, J. (1995). A simple and accurate method for 3-D measurements in microcorrosion casts illustrated with tumour vascularization. Anal Cell Pathol 9, 69–81.Google Scholar
Miodonski, A., Kus, J., Olszewski, E., & Tyrankiewicz, R. (1980). Scanning electron microscopic studies on blood vessels in cancer of the larynx. Arch Otolaryngol 106, 321–332.Google Scholar
Plate, K.H., Breier, G., Weich, H.A., & Risau, W. (1992). Vascular endothelial growth factor is a potential tumour angiogenesis factor in human gliomas in vivo. Nature 359, 845–847.Google Scholar
Schoenfeld, A., Levavi, H., Breslavski, D., Amir, R., & Ovadia, J. (1994). Three-dimensional modeling of tumor-induced ovarian angiogenesis. Cancer Lett 87, 79–84.Google Scholar
Skinner, S.A., Frydman, G.M., & O'brien, P.E. (1995). Microvascular structure of benign and malignant tumors of the colon in humans. Dig Dis Sci 40, 373–384.Google Scholar
Strömblad, S. & Cheresh, D.A. (1996). Cell adhesion and angiogenesis. Trends Cell Biol 6, 462–468.Google Scholar
Sundberg, C., Nagy, J.A., Brown, L.F., Feng, D., Eckelhoefer, I.A., Manseau, E.J., Dvorak, & A.M., Dvorak H.F. (2001). Glomeruloid microvascular proliferation follows adenoviral vascular permeability factor/vascular endothelial growth factor-164 gene delivery. Am J Pathol 158, 1145–1160.Google Scholar
Sunderkötter, C., Steinbrink, K., Goebeler, M., Bhardwaj, R., & Sorg, C. (1994). Macrophages and angiogenesis. J Leukoc Biol 55, 410–422.Google Scholar
Tatematsu, M., Cohen, S.M., Fukushima, S., Shirai, T., Shinohara, Y., & Ito, N. (1978). Neovascularization in benign and malignant urinary bladder epithelial proliferative lesions of the rat observed in situ by scanning electron microscopy and autoradiography. Cancer Res 38, 1792–1800.Google Scholar
Tsunenari, I. (1993). Cushion-like structure in coronary arteries of rats. Acta Anat Nippon 68, 67–75 (in Japanese with English summary).Google Scholar
Tsunenari, I., Yamate, J., & Sakuma, S. (1997). Poorly differentiated carcinoma of the parotid gland in a six-week-old Sprague–Dawley rat. Toxicol Pathol 25, 277–280.Google Scholar
Tsunenari, I., Yamate, J., Sharma, K.D., Kawachi, M., & Sakuma, S. (2000). Expressions of vascular endothelial growth factor and basic fibroblast growth factor in tumors induced by two different cloned cell lines established from transplantable rat malignant fibrous histiocytoma. J Vet Med Sci 62, 699–705.Google Scholar
Vaupel, P. & Gabbert, H. (1986). Evidence for and against a tumor type-specific vascularity. Strahlenther Onkol 162, 633–638.Google Scholar
Yamate, J., Tajima, M., Shibuya, K., Ihara, M., & Kudow, S. (1989). Morphologic characteristics of a transplantable tumor derived from a spontaneous malignant fibrous histiocytoma in the rat. Jpn J Vet Sci 51, 587–596.Google Scholar
Yamate, J., Tajima, M., Togo, M., Shibuya, K., Ihara, M., & Kudow, S. (1991). Heterogeneity of cloned cell lines established from a transplantable rat malignant fibrous histiocytoma. Jpn J Cancer Res 82, 298–307.Google Scholar
Zama, A., Tamura, M., & Inoue, H.K. (1991). Three-dimensional observations on microvascular growth in rat glioma using a vascular casting method. J Cancer Res Clin Oncol 117, 396–402.Google Scholar