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Angioarchitecture of Tumors Induced by Two Different Cloned Cell Lines Established from a Transplantable Rat Malignant Fibrous Histiocytoma

Published online by Cambridge University Press:  21 November 2003

Ichiro Tsunenari ,
Jyoji Yamate ,
Masae Iwaki ,
Mitsuru Kuwamura ,
Takao Kotani  and
Sadashige Sakuma
Ichiro Tsunenari
Affiliation:
Department of Toxicology and Safety Assessment, Kawanishi Pharma Research Institute, Nippon Boehringer Ingelheim Co., Ltd., 3-10-1 Yato, Kawanishi, Hyogo 666-0193, Japan
Jyoji Yamate
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Masae Iwaki
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Mitsuru Kuwamura
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Takao Kotani
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Sadashige Sakuma
Affiliation:
Department of Veterinary Pathology, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
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Abstract

Angiogenesis, a biologic process whereby endothelial cells divide and migrate to form new blood vessels, is a key step in tumor growth, invasion, and metastasis. In the present study, we investigated the differences in angioarchitecture between two different tumors induced by cloned cell lines (MT-8 and MT-9), derived from a transplantable rat malignant fibrous histiocytoma, by scanning electron microscopy of vascular corrosion casts. During a 3-week observation period after implantation, the growth of MT-8 tumors appeared to be faster than that of MT-9 tumors. Histologically, MT-8 tumors were of the uniformly undifferentiated sarcoma type arranged in characteristic organoid structures, and MT-9 tumors showed a storiform growth pattern. In MT-8 tumors, neovascularization occurred by sprouting at postimplantation (PI) week 1, and the newly formed capillaries gradually became more tortuous. In MT-9 tumors, at PI week 1, the corrosion casts of newly formed capillaries mainly showed a wavy course but no fingerlike outgrowths of capillaries were seen. At PI weeks 2 and 3, the sprouting was seen specifically in MT-9 tumors, forming basketlike structures and glomeruloid structures of capillaries. These results indicate that angiogenesis or angioarchitecture of MT-8 tumors is different from that of MT-9 tumors, depending on the differences in their tumor histology and by the features like absence or presence of basketlike structures and glomeruloid structures of capillaries.

Keywords

angioarchitecturetumor angiogenesismalignant fibrous histiocytomatransplantationcorrosion castingrat
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 9 , Issue 6 , December 2003 , pp. 532 - 541
Copyright
© 2003 Microscopy Society of America

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