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Analysis of Orientations of Collagen Fibers by Novel Fiber-Tracking Software

Published online by Cambridge University Press:  21 November 2003

Jun Wu ,
Bartłomiej Rajwa ,
David L. Filmer ,
Christoph M. Hoffmann ,
Bo Yuan ,
Ching-Shoei Chiang ,
Jennie Sturgis  and
J. Paul Robinson
Jun Wu
Affiliation:
Department of Biology, Purdue University, West Lafayette, IN 47907, USA
Bartłomiej Rajwa
Affiliation:
Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907, USA Department of Biophysics, The Jan Zurzycki Institute of Molecular Biology and Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-387 Kraków, Poland
David L. Filmer
Affiliation:
Department of Biology, Purdue University, West Lafayette, IN 47907, USA
Christoph M. Hoffmann
Affiliation:
Department of Computer Science, Purdue University, West Lafayette, IN 47907, USA
Bo Yuan
Affiliation:
Department of Computer Science, Purdue University, West Lafayette, IN 47907, USA
Ching-Shoei Chiang
Affiliation:
Department of Computer Science, Purdue University, West Lafayette, IN 47907, USA
Jennie Sturgis
Affiliation:
Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907, USA
J. Paul Robinson
Affiliation:
Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907, USA Department of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
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Abstract

Recent evidence supports the notion that biological functions of extracellular matrix (ECM) are highly correlated to not only its composition but also its structure. This article integrates confocal microscopy imaging and image-processing techniques to analyze the microstructural properties of ECM. This report describes a two- and three-dimensional fiber middle-line tracing algorithm that may be used to quantify collagen fibril organization. We utilized computer simulation and statistical analysis to validate the developed algorithm. These algorithms were applied to confocal images of collagen gels made with reconstituted bovine collagen type I, to demonstrate the computation of orientations of individual fibers.

Keywords

image processingconfocal microscopyECMcollagen fibersorientation
Type
Microscopy Techniques
Information
Microscopy and Microanalysis , Volume 9 , Issue 6 , December 2003 , pp. 574 - 580
Copyright
© 2003 Microscopy Society of America

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