Over the course of the nineteenth century, major changes transformed the transport of people and freight in Rio de Janeiro, the capital of Brazil during this period. These transformations involved both technological change, as transport evolved first from carriages and carts to horse-drawn trams and then to electric trams, as well as economic developments, such as the establishment of the first tram companies, many of which became important vehicles for foreign capital to enter Brazil. Although there has been extensive research from various angles into the changes undergone by the city's transport sector, there remains, however, a significant lacuna in the existing literature: the workers involved in that sector. The aim of this article is to analyse the workforce of the urban transport sector in Rio de Janeiro in the nineteenth century, and to understand the labour that these workers provided, how they were affected by the transformations in the sector, and, at the same time, how they responded to those transformations. During this period, issues such as the connections between free and unfree labour, ethnic conflicts, and work regulation were very important in transport work in Rio de Janeiro, and they are explored in the text.