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Accepted manuscript

Effects of Multi-species Synbiotic Supplementation on Circulating miR-27a, miR-33a Levels, and Lipid Parameters in Adult Men with Dyslipidemia; A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

Published online by Cambridge University Press:  30 April 2024

Shekoufeh Salamat Open the ORCID record for Shekoufeh Salamat [Opens in a new window] ,
Alireza Jahan-Mihan Open the ORCID record for Alireza Jahan-Mihan [Opens in a new window] ,
Mohammad Reza Tabandeh  and
Anahita Mansoori
Shekoufeh Salamat
Affiliation:
Nutrition and Metabolic Diseases Research Center, Clinical Sciences Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Alireza Jahan-Mihan
Affiliation:
Department of Nutrition and Dietetics, University of North Florida, Jacksonville, FL, USA
Mohammad Reza Tabandeh
Affiliation:
Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran Stem Cells and Transgenic Technology Research Center, Shahid Chamran University of Ahvaz, Iran
Anahita Mansoori*
Affiliation:
Nutrition and Metabolic Diseases Research Center, Clinical Sciences Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
*
*Corresponding author: Dr. Anahita Mansoori, Email address: [email protected], Nutrition Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Postal code: 61357-15794, Fax and Tel: (+9861)33738330, (+9861)33738253
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Abstract

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MicroRNAs (miRNAs) have emerged as important regulators of lipid metabolism. Recent studies have suggested synbiotics may modulate miRNA expression and lipid metabolism. This study aimed to investigate the effects of synbiotic supplementation on circulating miR-27a, miR-33a, and lipid parameters in patients with dyslipidemia. Fifty-six eligible participants were randomly allocated to receive either synbiotic or placebo sachets twice a day for 12 weeks. Each synbiotic sachet contained 3×1010 CFU six species of probiotic microorganisms and 5 grams of inulin and fructooligosaccharide (FOS) as prebiotics. Serum miR-27a and miR-33a expression levels, serum lipids, and apolipoproteins, the fecal concentration of short-chain fatty acids (SCFAs), and Firmicutes and Bacteroidetes phyla were assessed before and after the study. Real-time PCR was used to determine the relative expression levels of miRNAs. The results showed synbiotic supplementation significantly downregulated the expression levels of miR-27a and miR-33a compared to the placebo group (p = 0.008 and p = 0.001, respectively). Furthermore, the intervention group exhibited significant improvements in serum high-density lipoprotein (HDL-C), small dense low-density lipoprotein (sdLDL-C), apoA-I, and apoB-100 (p = 0.008, p = 0.006, p = 0.003, p = 0.001, respectively). The results showed a significant negative correlation between miR-33a expression levels with HDL-C, butyrate, propionate, and a significant positive correlation with total cholesterol (TC), low-density lipoprotein (LDL-C), and sdLDL-C in the intervention group. Fecal bacteria and SCFAs were significantly increased in the intervention group. This study provides evidence that synbiotic supplementation can modulate miR-27a and miR-33a expression and improve lipid metabolism in patients with dyslipidemia.

Keywords

SynbioticDyslipidemiaMicroRNAGut microbiotaLipid profile
Type
Research Article
Information
British Journal of Nutrition , Accepted manuscript , pp. 1 - 26
Copyright
© The Authors 2024