Book contents
- Frontmatter
- Contents
- Preface
- Acknowledgments
- 1 Introduction to Fed-Batch Cultures
- 2 Idealized Reactors and Fed-Batch Reactors
- 3 Maximization of Reaction Rates and Fed-Batch Operation
- 4 Phenomena That Favor Fed-Batch Operations
- 5 Classification and Characteristics of Fed-Batch Cultures
- 6 Models Based on Mass Balance Equations
- 7 Non–Equation-Based Models
- 8 Specific Rate Determination
- 9 Optimization by Pontryagin's Maximum Principle
- 10 Computational Techniques
- 11 Optimization of Single and Multiple Reactions
- 12 Optimization for Cell Mass Production
- 13 Optimization for Metabolite Production
- 14 Simple Adaptive Optimization
- 15 Measurements, Estimation, and Control
- 16 Feasibility Assessment and Implementable Feed Rates
- Index
Preface
Published online by Cambridge University Press: 05 April 2013
- Frontmatter
- Contents
- Preface
- Acknowledgments
- 1 Introduction to Fed-Batch Cultures
- 2 Idealized Reactors and Fed-Batch Reactors
- 3 Maximization of Reaction Rates and Fed-Batch Operation
- 4 Phenomena That Favor Fed-Batch Operations
- 5 Classification and Characteristics of Fed-Batch Cultures
- 6 Models Based on Mass Balance Equations
- 7 Non–Equation-Based Models
- 8 Specific Rate Determination
- 9 Optimization by Pontryagin's Maximum Principle
- 10 Computational Techniques
- 11 Optimization of Single and Multiple Reactions
- 12 Optimization for Cell Mass Production
- 13 Optimization for Metabolite Production
- 14 Simple Adaptive Optimization
- 15 Measurements, Estimation, and Control
- 16 Feasibility Assessment and Implementable Feed Rates
- Index
Summary
Fed-batch operations are semi-batch operations in which one or more streams of feed containing nutrient sources, precursors, inducers, and mineral sources are fed either continuously or intermittently during the course of otherwise batch operations. The culture content is harvested either fully or partially at the end of the run and is used as the inoculum for the next cycle. By regulating the feed rates, it is possible to regulate the bioreactor environment to maximize the total rate of production, the reactor productivity, or the product yield.
Many industrially important bioreactor operations involving microbial and animal cells are carried out in fed-batch mode. These so-called fed-batch cultures have been found to be particularly effective for fermentation processes and cell cultures in which it is desirable to overcome such common phenomena as substrate inhibition, catabolite repression, product inhibition, and glucose effects to achieve high cell density for efficient fermentation, to minimize high viscosity effects, and to take advantage of auxotrophic mutants. Products produced by fed-batch cultures include amino acids, antibiotics, enzymes, microbial cells, organic chemicals, polysaccharides, proteins, tissue culture products, and various recombinant DNA products.
- Type
- Chapter
- Information
- Fed-Batch CulturesPrinciples and Applications of Semi-Batch Bioreactors, pp. xv - xviPublisher: Cambridge University PressPrint publication year: 2013