Skip to main content Accessibility help
×
Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-09T22:46:53.260Z Has data issue: false hasContentIssue false

18 - A Misclassification Model for Inferring Transcriptional Regulatory Networks

Published online by Cambridge University Press:  23 November 2009

Kim-Anh Do
Affiliation:
University of Texas, MD Anderson Cancer Center
Peter Müller
Affiliation:
Swiss Federal Institute of Technology, Zürich
Marina Vannucci
Affiliation:
Rice University, Houston
Get access

Summary

Abstract

One major goal in biological research is to understand how genes are regulated through transcriptional regulatory networks. Recent advances in biotechnology have generated enormous amounts of data that can be utilized to better achieve this goal. In this chapter, we develop a general statistical framework to integrate different data sources for transcriptional regulatory network reconstructions. More specifically, we apply measurement error models for network reconstructions using both gene expression data and protein–DNA binding data. A linear misclassification model is used to describe the relationship between the expression level of a specific gene and the binding activities of the proteins (transcription factors) that regulate this gene. We propose Markov chain Monte Carlo method for statistical inference based on this model. Extensive simulations are conducted to evaluate the performance of this model and assess the sensitivity of its performance when the model parameters are misspecified. Our simulation results suggest that our approach can effectively integrate gene expression data and protein–DNA binding data to infer transcriptional regulatory networks. Lastly, we apply our model to jointly analyze gene expression data and protein–DNA binding data to infer transcriptional regulatory networks in the yeast cell cycle.

Introduction

Understanding gene regulations through the underlying transcriptional regulatory networks (referred as TRNs in the following) is a central topic in biology. A TRN can be thought of as consisting of a set of proteins, genes, small modules, and their mutual regulatory interactions. The potentially large number of components, the high connectivity among various components, and the transient stimulation in the network result in great complexity of TRNs.

Type
Chapter
Information
Publisher: Cambridge University Press
Print publication year: 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Save book to Kindle

To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Available formats
×

Save book to Dropbox

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox.

Available formats
×

Save book to Google Drive

To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive.

Available formats
×