Herbicide resistance has increased the need for novel weed control strategies. Fluridone has herbicidal as well as potential germination stimulant activity. The objectives of this study were to evaluate fluridone as a fall-applied germination stimulant for weed control and to assess rotational crop tolerance. Fall-applied fluridone was compared with a nontreated control in areas established with false cleavers, volunteer canola, and wild oat at Lacombe, AB, in 2014–2015 and 2015–2016, and at St Albert, AB, in 2015–2016. In the fall, there was a trend for weed densities to be higher in fluridone treatments than in untreated controls across site-years. The stimulatory effect of fluridone on weed germination was not statistically significant in fall assessments, while the weed control effect was significant in 33% of spring assessments. While fluridone reduced weed biomass for some site-years, it also reduced canola crop emergence and biomass at St Albert in 2015–2016, and caused injury symptoms on wheat and field pea. Risk of carryover to subsequent crops outweighed the benefits of using fluridone in the fall to stimulate weed germination in this study.

As chemical management options for weeds become increasingly limited due to selection for herbicide resistance, investigation of additional nonchemical tools becomes necessary. Harvest weed seed control (HWSC) is a methodology of weed management that targets and destroys weed seeds that are otherwise dispersed by harvesters following threshing. It is not known whether problem weeds in western Canada retain their seeds in sufficient quantities until harvest at a height suitable for collection. A study was conducted at three sites over 2 yr to determine whether retention and height criteria were met by wild oat, false cleavers, and volunteer canola. Wild oat consistently shed seeds early, but seed retention was variable, averaging 56% at the time of wheat swathing, with continued losses until direct harvest of wheat and fababean. The majority of retained seeds were >45 cm above ground level, suitable for collection. Cleavers seed retention was highly variable by site-year, but generally greater than wild oat. The majority of seed was retained >15 cm above ground level and would be considered collectable. Canola seed typically had >95% retention, with the majority of seed retained >15 cm above ground level. The suitability ranking of the species for management with HWSC was canola>cleavers>wild oat. Efficacy of HWSC systems in western Canada will depend on the target species and site- and year-specific environmental conditions.

Two furrow irrigated field experiments were conducted for two years at the Research and Extension Center, Powell, WY to determine the influence of various mixed densities and durations of wild oat and wild mustard interference in sugarbeet. Sugarbeet root yields were reduced by competition from all examined densities of wild oat and wild mustard, alone and in combination. Root yield reduction was less than additive with mixed densities of wild oat and wild mustard. Root yields decreased as the duration of interference after sugarbeet emergence from a mixed density of wild oat and wild mustard increased. Sucrose content of sugarbeet was not altered by competition. Based on regression analysis, the minimum time that a mixed density of 0.8 wild mustard and 1 wild oat/m of row can interfere with sugarbeet before causing an economic root yield loss was approximately 1.6 weeks after sugarbeet emergence.

The efficacy of current wild oat herbicides and their high cost have resulted in use rates that are less than those recommended. While acceptable weed control may be attained at less cost, this practice does not consider the potential for increased wild oat seed production. The objective of this experiment was to determine the interaction of wild oat density and reduced imazamethabenz rates on wild oat seed production in spring barley. As wild oat densities increased from 8 to 1,100 plants m−2, wild oat seed production increased from 180 to 9,950 seed m−2 without herbicide, and from 0 to 2,810 seed m−2 using 0.53 kg ai ha−1 imazamethabenz. This general pattern was modeled using a cumulative logistic function. Estimates from this model indicated that < 1 wild oat seed m−2 was produced at population densities of ≤ 20 plants at any imazamethabenz rate. Imazamethabenz rates of 0.26 kg ha−1 or greater at wild oat densities of less than approximately 190 plants m−2 did not result in wild oat seed production above the initial population density. As wild oat density increased, however, imazamethabenz rates below 0.40 kg ha−1 resulted in substantially greater wild oat seed production compared to the recommended rate.

The purpose of this study was to determine cross-resistance patterns among wild oat lines resistant to acetyl-CoA carboxylase (ACCase) inhibitors and to determine which, if any, cross-resistant type was more common than another. Discriminatory concentrations of two aryloxyphenoxy-propionates (APP) and three cyclohexanediones (CHD) were determined using a petri-dish bioassay. These concentrations were then applied to 82 resistant wild oat lines identified in previous studies. In addition, two resistant standards (UM1 and UM33) and a susceptible standard (UM5) were included in the experiments. Coleoptile lengths expressed as percentages of untreated controls were used to assess the level of resistance to each herbicide. Large variations were observed among wild oat lines and herbicides. However, cluster analysis summarized the relationship between the five herbicides (variables) and the wild oat lines into three main cross-resistance types. Type A included wild oat lines with high resistance to APP herbicides and no or low resistance to CHD herbicides. Types B and C included those with low to moderate resistant and high levels of resistance to all five herbicides, respectively. Type C was the most common cross-resistance type. Relationships among herbicides were determined using pairwise correlation and principal component analysis (PCA). All correlations were high between APP herbicides and between CHD herbicides but not between APP and CHD herbicides. The first two axes of the PCA accounted for 88.4% of the total variance, with the first axis correlated to the CHD herbicides and the second axis correlated to the APP herbicides. In the PCA, wild oat lines were segregated into the three types identified in the cluster analysis. Although CHD and APP herbicides bind at the same region on the ACCase, resistant wild oat lines respond differently to them.

The development of problematic weed populations is a concern in western Canadian fields where canola and pea are grown in a 4-yr sequence with spring cereal grains. Weed densities were examined at a site near Melfort, Saskatchewan, Canada, from 1994 to 1997 in seven zero-till managed crop rotations. Four rotations that included canola, pea, or flax in at least 3 of 4 yr (HBF: high broadleaf–crop frequency) were compared with three rotations that included broadleaf crops grown in 2 of 4 yr (LBF: low broadleaf–crop frequency). Spring wheat and barley were the cereal crops in rotation. Residual (postherbicide application) weed density for each weed species in a given year was summed across all phases for each rotation to reflect the overall weed infestation. Four annual broadleaf weed species were most abundant in 1996 and a second group of three species, having a variety of reproductive strategies, became progressively less abundant as the study progressed. The difference between the HBF and LBF rotations for the density of these species varied and was most prominent in years when environmental conditions were conducive for their growth. More frequent applications of ethafluralin, with its residual weed control, best explained why wild oat and catchweed bedstraw generally were less abundant in the HBF rotations. Of particular interest was the 8 plants m−-2 greater density of dandelion and perennial sowthistle in the HBF vs. LBF rotations in the last year of the study. It is thought that the limited herbicide options for the control of these species could present a future problem if they continued to develop in the HBF rotations. Differences in herbicide use between the HBF and LBF rotations were considered the primary factor controlling the rotation effects on weed density.

Harvest weed seed control (HWSC) systems have been developed to exploit the high proportions of seed retained at maturity by the annual weeds rigid ryegrass, wild radish, bromegrass, and wild oats. To evaluate the efficacy of HWSC systems on rigid ryegrass populations, three systems, the Harrington Seed Destructor (HSD), chaff carts, and narrow-windrow burning were compared at 24 sites across the western and southern wheat production regions of Australia. HWSC treatments were established at harvest (Nov. – Dec.) in wheat crops with low to moderate rigid ryegrass densities (1 to 26 plants m−2). Rigid ryegrass counts at the commencement of the next growing season (Apr. – May) determined that HWSC treatments were similarly effective in reducing emergence. Chaff carts, narrow-windrow burning, or HSD systems act similarly on rigid ryegrass seed collected during harvest to deliver substantial reductions in subsequent rigid ryegrass populations by restricting seedbank inputs. On average, population densities were reduced by 60%, but there was considerable variation between sites (37 to 90%) as influenced by seed production and the residual seedbank. Given the observed high rigid ryegrass seed production levels at crop maturity it is clear that HWSC has a vital role in preventing seedbank inputs in Australian conservation cropping systems.

Information on nitrogen fertilizer effects on crop–weed competitive interactions might aid in developing improved weed management programs. A controlled environment study was conducted to examine the effect of three N rates on the competitive ability of four weed species grown with wheat. The four weed species were chosen to represent species that varied in their growth responsiveness to nitrogen (N): Persian darnel (low), Russian thistle (low), redroot pigweed (high), and wild oat (high). Wheat and each weed species were grown in a replacement series design at N rates of 60, 120, and 240 mg N kg−1 soil. The competitive ability of the low N-responsive species, Persian darnel and Russian thistle, was not influenced by N rate, supporting our hypothesis that N rate would have little effect on the competitiveness of species responding minimally to N. Conversely, the competitiveness of the high N-responsive species redroot pigweed progressively improved as N rate increased. However, wild oat competitiveness was unaffected by N fertilizer rate. There is some evidence from this study to suggest that fertilizer management strategies that favor crops over weeds deserve greater attention when weed infestations consist of species known to be highly responsive to higher soil N levels. Information gained in this study will be used to advise farmers of the importance of strategic fertilizer management in terms of both weed management and crop yield.

Dormancy is a critical factor for the survival and persistence of weedy species. Contemporary approaches can be used to identify genes that regulate dormancy directly or indirectly to elucidate key mechanisms, signals, and pathways. Several domesticated plant species have been used as model systems to mark quantitative trait loci (QTL) that affect dormancy in seeds and vegetative propagules directly. A few weedy species have also been used to mark QTL and to determine dormancy genes using microarray analysis. Given the number of serious weeds worldwide and the role that dormancy plays in their persistence, developing fundamental knowledge on dormancy is an important step toward developing new strategies for weed management. This paper describes current research and outlines some weeds that might be candidates for dormancy investigations using molecular genetic and genomics approaches. An underlying theme in the selection of weeds for dormancy investigations is their relation to crop species and the ability to adapt existing resources to investigate dormancy in weedy plants.

Wild oat continues to reduce spring wheat yields and profits despite the wide spread use of herbicides. Further reductions in the occurrence of wild oat could be achieved with the development of competitive cropping systems. Field studies were conducted to investigate the effects of wheat seed size and seeding rate on wild oat demographic processes under a range of wild oat densities. Spring wheat competitiveness increased as seed size and seeding rate increased, significantly reducing wild oat biomass and seed production. Averaged across all other factors, spring wheat plants derived from large seed reduced wild oat panicle numbers 15% and biomass and seed production 25% compared with small seed. Increasing spring wheat seeding rate from 175 to 280 plants m−2 reduced the number of panicles 10% and wild oat biomass and seed production 20%. The combined effect of large seed plus increased seeding rate reduced wild oat biomass and seed production 45%. Results demonstrate that the use of large seed size and increased seeding rates can improve wheat competitiveness and provide an effective means to reduce wild oat biomass and seed production.

In Europe, 18 weedy grass species had been confirmed to have biotypes with resistance to herbicides. The most frequent is that of atrazine resistance, with nine resistant biotypes found. These biotypes are mainly resistant because of changes in the D1 protein of photosystem II. All atrazine-resistant biotypes, except that of bristly foxtail, show cross-resistance to s-triazine and as-triazines. From an agriculture point of view, the most important cases of resistance are those found in blackgrass, wild oat, Italian ryegrass, rigid ryegrass, and barnyardgrass. In these species, cross- and multiple resistances were observed due to metabolism or changes in the target protein by genetic mutations or both. These biotypes are extremely difficult to control with alternative herbicides.

Weed seedling emergence is partially dependent on biotic and abiotic conditions directly surrounding the seed. When environmental conditions are appropriate, seed germination and emergence occurs. We studied the impact of seeding depth (surface, 1 to 2, 3 to 4, and 6 to 7 cm) and fluctuating soil moisture regimes (field capacity [FC]–1/3FC–FC; FC–1/6FC–FC) on percent weed emergence in a greenhouse. At FC, wild mustard and field pennycress had the greatest percent emergence when seeds were placed on or near the soil surface, whereas percent emergence of barnyardgrass and round-leaved mallow was unaffected by seeding depth. All the perennials tested had the greatest percent emergence at FC when seeds were placed near or on the soil surface, except for common milkweed which only emerged below the soil surface. When soil moisture levels fluctuated, surface seeds of barnyardgrass, catchweed bedstraw, green foxtail, wheat, and wild oat had less emergence than seeds below the soil surface; field pennycress had increased emergence when the seeds were placed on the surface; and round-leaved mallow and wild mustard emergence was unaffected by seeding depth. The emergence of curly dock, dandelion, and perennial sowthistle was unaffected by seeding depth, whereas foxtail barley and quackgrass emergence was reduced when seeds were placed on the surface and soil moisture fluctuated.

Combine harvesters have the potential to disperse weed seeds great distances. Reducing this dispersal may be important in an integrated weed management system. The objectives of this study were to determine the distance that wild oat seeds are dispersed by a combine harvester and the effect of chaff collection on combine harvester seed dispersal. This was measured by sampling wild oat seeds at varying distances behind a combine equipped with a removable chaff collection system after it passed through a wild oat patch. Chaff collection consistently reduced the amount and distance that wild oat seeds were dispersed. This occurred because more than 74% of the total wild oat seed that were ejected from the combine were in the chaff. Because most of the chaff falls in a row directly behind the combine, chaff collection only affected dispersal in this area. In 1996, chaff collection reduced wild oat seed dispersal past the wild oat patch to less than 10 seeds m−2 at 45 m, whereas without chaff collection, there was greater than 10 seeds m−2 up to 145 m. At distances beyond 145 m, chaff collection had no significant effect on seed dispersal. Chaff collection may be an important tool in an integrated weed management program because it may slow weed invasions and reduce the expansion of weed patches.

Wild oat, a troublesome weed in small grain cereals, infests about 11 million ha of cropland in the United States. Diversifying cereal production with alternative crops, such as yellow mustard and canola, provides flexible cropping systems, decreases production risks, and may allow more effective weed suppression. A greenhouse study was conducted to assess the competitive ability of yellow mustard and canola with wild oat in 1999 and 2000, using replacement series interference experiments to relate the results to plant development stages. Yellow mustard, regardless of its proportion in mixture, reduced aboveground biomass of wild oat 33 to 66%, leaf and tiller number 34 to 36%, and panicle production 58% compared with wild oat in monoculture. Canola did not affect wild oat biomass in mixtures. Yellow mustard per plant biomass in 2000 and inflorescence production in 1999 decreased 30 and 20% with increased density of yellow mustard in mixtures. Yellow mustard biomass was not affected by the addition of wild oat to the mixture, indicating the greater importance of intraspecific competition between yellow mustard relative to interspecific competition with wild oat. Canola per plant biomass was affected more by interspecific competition with wild oat than by intraspecific competition. A second greenhouse experiment was conducted to compare plant height and biomass accumulation by the three species over 7 wk. Yellow mustard had the greatest biomass accumulation and plant elongation rate, followed by canola and wild oat. The greater competitive ability of yellow mustard with wild oat, compared with canola, is likely associated with the rapid growth and canopy elevation of yellow mustard.

Field trials were initiated in fall 2011 to determine the potential of pyroxasulfone to control acetolactate synthase (ALS) inhibitor-resistant weeds in field pea. Pyroxasulfone was applied in split-plot trials at five locations in western Canada using fall and PRE spring applications of 0 to 400 g ai ha−1. Trial locations were chosen with a range of soil organic matter content: 2.9, 4.3, 5.5, 10.5, and 10.6% at Scott, Kernen, Kinsella, Melfort, and Ellerslie, respectively. The herbicide dose required to reduce biomass by 50% (ED50) in false cleavers ranged between 53 and 395 g ha−1 at Scott and Ellerslie, respectively. Wild oat ED50s varied between 0.54 g ha−1 at Scott in the fall and 410 g ai ha−1 in the spring at Melfort. ED50s for wild oat and false cleavers varied by 7.4- and 746-fold, respectively, depending primarily on the organic matter content at the trial location. The effect of application timing was not consistent. Significant yield reductions and pea injury occurred at 150 and 100 g ha−1 and higher at Kernen and Scott, respectively. Low organic matter and high precipitation levels at these locations indicates increased herbicide activity under these conditions. Pyroxasulfone may allow control of ALS inhibitor-resistant false cleavers and wild oat; however, locations with high soil organic matter will require higher rates than those with low organic matter for similar control levels.

Arabidopsis complementary DNA (cDNA) microarrays were hybridized with labeled cDNA from mature leaves and emerging tillers of wild oat to determine if they could identify gene expression profiles in distantly related species. More than 23% of the > 11,000 cDNAs on the array hybridized to the wild oat probe. Transcription patterns detected by hybridization to the arrays are indicators for physiological processes in the tissues tested. Coordinated expression patterns for these genes in Arabidopsis indicate common signals involved in their regulation. The results demonstrate that probing cDNA-based arrays from well-characterized species can provide valuable insight into the signal transduction processes regulating growth and development of poorly characterized species.

Site-specific fertilizer application is of primary interest among those investigating site-specific crop management. Site-specific soil moisture levels may be associated with the relative success of certain weed species, and weed competition can be affected by fertilizer application. A field study was conducted to investigate how landscape position–based site-specific nitrogen fertilizer application would influence wild oat interference in spring wheat. Wild oat was allowed to grow in spring wheat at foot and knoll landscape positions in the presence or absence of 80 to 90 kg ha−1 of spring preseed broadcast nitrogen fertilizer. In all three site–years of the study, landscape position did not affect wild oat competitiveness in wheat or relative wild oat biomass, and there were no significant landscape position by nitrogen fertilizer interactions for these variables. The lack of landscape position effect may be attributed to the lack of substantive differences in soil characteristics between landscape positions at the sites used in this study and to normal to above-average seasonal precipitation levels in all three site–years. Wild oat competitiveness in wheat was significantly greater in the presence of nitrogen fertilizer for all three site–years. The results of this study suggest that, when there are not great differences in typical soil characteristics between landscape positions and precipitation levels are normal or above normal, landscape-based site-specific nitrogen fertilizer application does not affect wild oat competitiveness in spring wheat, but preseed spring broadcast nitrogen fertilizer does make wild oat more competitive in spring wheat.

Seed production of annual weeds persisting through cropping phases replenishes/establishes viable seed banks from which these weeds will continue to interfere with crop production. Harvest weed seed control (HWSC) systems are now viewed as an effective means of interrupting this process by targeting mature weed seed, preventing seed bank inputs. However, the efficacy of these systems is directly related to the proportion of total seed production that the targeted weed species retains (seed retention) at crop maturity. This study determined the seed retention of the four dominant annual weeds of Australian cropping systems - annual ryegrass, wild radish, brome grass, and wild oat. Beginning at the first opportunity for wheat harvest and on a weekly basis for 28 d afterwards the proportion of total seed production retained above a 15 cm harvest cutting height was determined for these weed species present in wheat crops at nine locations across the Western Australian (WA) wheat-belt. Very high proportions of total seed production were retained at wheat crop maturity for annual ryegrass (85%), wild radish (99%), brome grass (77%), and wild oat (84%). Importantly, seed retention remained high for annual ryegrass and wild radish throughout the 28 d harvest period. At the end of this period, 63 and 79% of total seed production for annual ryegrass and wild radish respectively, was retained above harvest cutting height. However, seed retention for brome grass (41%) and wild oat (39%) was substantially lower after 28 d. High seed retention at crop maturity, as identified here, clearly indicates the potential for HWSC systems to reduce seed bank replenishment and diminish subsequent crop interference by the four most problematic species of Australian crops.

The environment in which a plant grows (maternal environment) can affect seed viability, germination, and dormancy. We assessed the effects of maternal environment on wild oat seed viability, germination, dormancy, and pathogen infection by collecting and analyzing wild oat seed from above and below a barley canopy at three field sites in Montana. The viability of wild oat seed collected below a crop canopy was consistently less than it was for seed from the overstory but varied among sites and years. Reductions in viability because of relative canopy position ranged from 10% to 30%. Effects of position relative to crop canopy on weed seed germination/dormancy rates varied by site and suggest that the direction and magnitude of the effects of maternal environment on dormancy depend on environmental conditions. These effects may be driven by crop competition or by changes in seed pathogen pressure or both. Seven species each of fungi and bacteria were isolated from wild oat seeds. The only fungi causing reductions in seed viability (15%) was isolated from understory seeds, and several bacteria from both overstory and understory sources reduced seed germination. Results suggest that, in addition to the known weed-suppressive effects of using taller or earlier emerging varieties of crops, such crops can reduce weed spread through effects on weed seed demography because weeds growing beneath the crop canopy produce a reduced amount of viable seed that is less likely to germinate in the following year.

Weed resistance monitoring has been routinely conducted in the Northern Great Plains of Canada (Prairies) since the mid-1990s. Most recently, random surveys were conducted in Alberta in 2001, Manitoba in 2002, and Saskatchewan in 2003 totaling nearly 800 fields. In addition, nearly 1,300 weed seed samples were submitted by growers across the Prairies between 1996 and 2006 for resistance testing. Collected or submitted samples were screened for group 1 [acetyl-CoA carboxylase (ACCase) inhibitor] and/or group 2 [acetolactate synthase (ALS) inhibitor] resistance. Twenty percent of 565 sampled fields had an herbicide-resistant (HR) wild oat biotype. Most populations exhibited broad cross-resistance across various classes of group 1 or group 2 herbicides. In Manitoba, 22% of 59 fields had group 1–HR green foxtail. Group 2–HR biotypes of kochia were documented in Saskatchewan, common chickweed and spiny sowthistle in Alberta, and green foxtail and redroot pigweed in Manitoba. Across the Prairies, HR weeds are estimated to occur in fields covering an area of nearly 5 million ha. Of 1,067 wild oat seed samples submitted by growers and industry for testing between 1996 and 2006, 725 were group 1 HR, 34 group 2 HR, and 55 groups 1 and 2 HR. Of 80 submitted green foxtail samples, 26 were confirmed group 1 HR; most populations originated from southern Manitoba where the weed is most abundant. Similar to the field surveys, various group 2–HR biotypes were confirmed among submitted samples: kochia, wild mustard, field pennycress, Galium spp., common chickweed, and common hempnettle. Information from grower questionnaires indicates patterns of herbicide usage are related to location, changing with cropping system. Two herbicide modes of action most prone to select resistance, groups 1 and 2, continue to be widely and repeatedly used. There is little evidence that growers are aware of the level of resistance within their fields, but a majority have adopted herbicide rotations to proactively or reactively manage HR weeds.