The transition to maize agriculture frames important cultural shifts in the Eastern Woodlands. However, the tempo and mode of this transition are unclear, particularly when analytical techniques are not standard across the region. In this article, we present evidence of directly dated maize macrobotanical fragments from the Turpin site in southwest Ohio that date between cal AD 552–649 and 684–994. These dates add to current dialogues on the spread of maize in the American Midcontinent and help further situate the Middle Ohio Valley as a cultural crossroads through which people and ideas flowed. We echo suggestions that, to refine our understanding of the introduction of maize into the region, we must develop pan-regional analytical standards and create multiple working hypotheses at a variety of scales.