Drift scan observations provide the broad sky coverage and instrumental stability needed to measure the Epoch of Reionization (EoR) 21-cm signal. In such observations, the telescope’s pointing centre (PC) moves continuously on the sky. The Tracking Tapered Gridded Estimator (TTGE) combines observations from different PC to estimate $P(k_{\perp}, k_{\parallel})$ the 21-cm power spectrum, centred on a tracking centre (TC) which remains fixed on the sky. The tapering further restricts the sky response to a small angular region around TC, thereby mitigating wide-field foregrounds. Here we consider $154.2\,\mathrm{MHz}$ ($z = 8.2$) Murchison Widefield Array (MWA) drift scan observations. The periodic pattern of flagged channels, present in MWA data, is known to introduce artefacts which pose a challenge for estimating $P(k_{\perp}, k_{\parallel})$. Here we have validated the TTGE using simulated MWA drift scan observations which incorporate the flagged channels same as the data. We demonstrate that the TTGE is able to recover $P(k_{\perp}, k_{\parallel})$ without any artefacts and estimate $P(k)$ within $5 \%$ accuracy over a large $k$-range. We also present preliminary results for a single PC, combining 9 nights of observation $(17 \, \mathrm{min}$ total). We find that $P(k_{\perp}, k_{\parallel})$ exhibits streaks at a fixed interval of $k_{\parallel}=0.29 \, \mathrm{Mpc}^{-1}$, which matches $\Delta \nu_\mathrm{per}=1.28 \, \mathrm{MHz}$ that is the period of the flagged channels. Since the simulations demonstrate that the TTGE is impervious to the flagged channels, the streaks seen for the actual data are possibly caused by some systematic that has the same period as the flagged channels. These streaks are more than 3–4 orders of magnitude smaller than the peak foreground power $\mid P(k_{\perp}, k_{\parallel}) \mid \approx 10^{16} \, \mathrm{mK^2}\, \mathrm{Mpc^3}$ at $k_{\parallel}=0$. The streaks are not as pronounced at larger $k_{\parallel}$, and in some cases they do not appear to extend across the entire $k_{\perp}$ range. The rectangular region $0.05 \leq k_{\perp} \leq 0.16 \, \mathrm{Mpc^{-1}}$ and $0.9 \leq k_{\parallel}\leq 4.6 \, \mathrm{Mpc^{-1}}$ is found to be relatively free of foreground contamination and artefacts, and we have used this to place the $2\unicode{x03C3}$ upper limit $\Delta^2(k) < (1.85\times10^4)^2\, \mathrm{mK^2}$ on the EoR 21-cm mean squared brightness temperature fluctuations at $k=1 \,\mathrm{Mpc}^{-1}$.

Midazolam is a short acting benzodiazepine that has been used for electroconvulsive therapy (ECT) anesthesia. The purpose of this study was to determine whether midazolam used for this purpose would impair the antidepressive efficacy of ECT. In a double-blind random-assignment study midazolam was compared to methohexital on the antidepressive efficacy of bilateral ECT as measured by the reduction in the Montgomery Asberg Depression Rating Scale (MADRS) scores and seizure duration. Sixteen DSM-III-R major depressive disorder patients with melancholia were included. Midazolam and methohexital did not differ in their effects on the MADRS score or seizure duration; no correlation was found between seizure duration and outcome of depression for either group. Our preliminary findings do not support the claim that benzodiazepines should not be used during bilateral ECT.

Gaga, a practice developed by Israeli choreographer Ohad Naharin, is one of the most popular training methods on the global dance market. Structured as a metatechnique, or a system for negotiating techniques within one's body, Gaga teaches students to both draw on and reject multiple movement techniques to create their own movement. I consider how the paradigms of choreography, technique, and improvisation are blurred together in the pedagogical model of a metatechnique and how training dancers to shift between choreographer, dancer, and improviser has significant ramifications for understanding their agency. The metatechnique model of Gaga falls in line with neoliberal values of efficiency and a wide range of skills and knowledge; this analysis provides an understanding of recent trends in dance training in relation to contemporary political and socioeconomic structures.

The sugarcane aphid Melanaphis sacchari (Zehnt.) has become a serious pest of sorghum, particularly during the post-rainy season in India and East and Southern Africa. Therefore, we tested a number of techniques to screen sorghum genotypes for their resistance to M. sacchari. Infesting the plants with aphid-infested leaf cuttings and covering with a nylon net was effective in screening sorghum genotypes for their resistance to M. sacchari. Sprinkling the plants with aphids (filled in an 0.5 ml eppendorf tube) in the greenhouse was also used to confirm whether the resistance of genotypes selected is less susceptible to the aphids under natural infestation. Nine genotypes (Line 61510, ICSV 12001, ICSV 12002, ICSV 12003, ICSV 12004, ICSV 12005, SLR 41, PU 10-1 and DJ 6514) exhibited moderate levels of resistance to M. sacchari. These genotypes also exhibited a lower rate of aphid multiplication in the clip cage and leaf disc assays. The rates of aphid multiplication were lower on the genotypes IS 21807, IS 40615, IS 40616 and IS 40618 than on the susceptible check, Swarna in the clip cage assay under the field conditions. Also, lower rates of aphid increase were also recorded on IS 21807 and IS 40615 in the leaf disc assay under laboratory conditions. Some of the genotypes that exhibited resistance to aphid damage under field conditions showed comparatively higher rates of aphid increase than the susceptible check, Swarna in the clip cage assay, indicating that antixenosis could be one of the components of resistance to M. sacchari in these genotypes. Therefore, the clip cage assay could be used to gain further understanding of the mechanisms of resistance to M. sacchari. There is a need to assess the role of antixenosis and colonization in genotypic reaction against M. sacchari to identify the lines with different mechanisms of resistance to this pest. The results suggested that the nylon net technique could be used to screen sorghum genotypes for resistance to M. sacchari. The genotypes exhibiting resistance to M. sacchari can be used to develop aphid-resistant sorghums for sustainable crop production.

Micrometeorological techniques can be applied to estimate methane (CH4) emissions from ruminants and livestock manure using CH4 concentration measured within the internal surface boundary layer. The main advantage of these techniques is that they are non-intrusive, thereby eliminating the impact of the measurement set-up on the calculated CH4 emission. This review focuses on four micrometeorological techniques, namely, the integrated horizontal flux (IHF), flux gradient (FG), eddy covariance (EC) and the dispersion modelling using the backward Lagrangian stochastic method (BLS). Each technique has unique advantages and limitations when used for estimating enteric (ruminant) and manure CH4 emissions. The IHF technique may be theoretically simpler then the FG, EC or BLS techniques, but all require high-resolution instruments to measure concentration. The EC and BLS techniques also require a measurement of the wind statistics. This review discusses the appropriate use of these four micrometeorological techniques for estimating CH4 emissions in animal agriculture and the recent advances in measurement technology.

The feasibility of feeding insects on different types of waste materials and subsequently feeding insects to poultry is reviewed. Research in this area has demonstrated that a number of insect taxa including silkworms, locusts, fly larvae, crickets and grasshoppers can be safely fed to chickens without compromising the quality and palatability of the meat. Most studies on the topic have dealt with which species of insects can be fed to chickens; they did not investigate rearing insects commercially which requires knowledge of insect rearing, feeding and production methods. This review examines this issue in greater depth by reviewing current literature and through discussions with the poultry farmers in the states of New South Wales and Queensland, Australia. A survey of poultry farmers found an acceptance of feeding insect to poultry provided it could be done economically and indicated that insects could be reared and fed on a variety of organic waste materials which are available in adequate amounts in different regions. The technique of feeding insects to poultry will be beneficial in developing agriculture based recycling systems, reducing waste and potentially aid in reducing environmental pollution. Depending on the insect species and whether the diet of insects is fed to meat producing or egg laying birds it will likely need to be supplemented with either or both calcium and limiting amino acids to meet the chicken's dietary requirement. The safety and economic viability of breeding and rearing insects on organic wastes and feeding to poultry needs to be assessed. In order to be economically feasible, the overall cost of rearing and feeding insects to poultry needs to be lower than the cost of feeding conventional protein sources like grains and soybean meal. The technique of feeding insects to poultry needs to be able to be easily adopted and readily adaptable by the industry to a range of farming systems. Further research needs to investigate these issues in more detail both by establishing small experiments and developing production models which include insects reared ‘onsite’ in the poultry diet.

Genetic manipulation allows simultaneous expression of green fluorescent protein (GFP) and its derivatives with a wide variety of cellular proteins in a variety of living systems. Epifluorescent and confocal laser scanning microscopy (confocal) localization of GFP constructs within living tissue and cell cultures has become routine, but correlation of light microscopy and high resolution transmission electron microscopy (TEM) on components within identical cells has been problematic. In this study, we describe an approach that specifically localizes the position of GFP/yellow fluorescent protein (YFP) constructs within the same cultured cell imaged in the confocal and transmission electron microscopes. We present a simplified method for delivering cell cultures expressing fluorescent fusion proteins into LR White embedding media, which allows excellent GFP/YFP detection and also high-resolution imaging in the TEM. Confocal images from 0.5-μm-thick sections are overlaid atop TEM images of the same cells collected from the next serial ultrathin section. The overlay is achieved in Adobe Photoshop by making the confocal image somewhat transparent, then carefully aligning features within the confocal image over the same features visible in the TEM image. The method requires no specialized specimen preparation equipment; specimens are taken from live cultures to embedding within 8 h, and confocal transmission overlay microscopy can be completed within a few hours.

Osmium postfixation is established as a routine procedure for transmission electron microscopy (TEM). On the one hand, this routine procedure leads to good results for TEM, but on the other hand results in blackened tissue samples that do not allow examination of any structures within the embedded tissue sample by a light microscope. Equivalent fixation results for TEM are achieved with imidazole–osmium postfixation, and with this postfixation method tissue is not blackened and can be transilluminated with point light sources. This allows easier recognition of histological details within tissue samples and makes it possible to screen embedded samples for appropriate ultrastructural processing. Jejunum is used to demonstrate the method.

Summary

Background and objective: Combined spinal–epidural (CSE) anaesthesia may be performed using separate needles or by passing the spinal needle through an epidural needle. The latter technique requires that subarachnoid block is performed before the epidural catheter is placed. This paper examines a series of 201 consecutive CSEs performed with a novel separate needle technique, designed to avoid potential and actual problems associated with the CSE technique.

Methods: The CSE technique involved placement of the spinal needle in the subarachnoid space, followed by replacement of the spinal needle stylet. The epidural catheter was then positioned separately before returning to the spinal needle and injecting the subarachnoid drug.

Results: The technique had a high technical success rate. Both needles were successfully placed in 200 (99.5%) cases. Spinal anaesthesia was successful in all cases. The epidural catheter was used in 179 cases and failure of the epidural occurred in 2 (1.1%) cases. Paraesthesia, inability to advance the epidural catheter or blood in the epidural catheter occurred in 31 (15.4%) and necessitated immediate replacement of the epidural catheter in 14 (7%) cases. Postoperatively, typical post-dural puncture headache was reported by one patient (0.5%) and mild backache by four (2%). There were no neurological complications.

Conclusions: This method of CSE anaesthesia can be associated with high success and low complication rates.