The objective of this study was to examine the effect of dietary vitamin A (retinol) on leptin in cattle. The relationship between plasma leptin and insulin was also examined. The experiment studies 16 9-month-old steers over the following 20-month fattening period. Four treatment diets were given to four steers in each of four groups. Cattle in treatment A were given a diet high in vitamin A (9000 to 10500 µg/day) throughout the experiment. Cattle in treatment B were given a diet low in vitamin A (300 to 4500 µg/day) for 0 to 10 months, after that they were given the high vitamin A diet during the period 10 to 20 months. Cattle in treatment C were given the low vitamin A diet for 0 to 14 months, after that they were given the high vitamin A diet for the period 14 to 20 months. Cattle in treatment D were given the low vitamin A diet throughout the experiment. Plasma samples were collected at 2-month intervals during the experiment to determine the plasma leptin, insulin and vitamin A concentrations. Plasma leptin concentration significantly increased over the fattening period (P < 0·001) but did not differ between the treatments (P > 0·05). Plasma leptin concentration was positively correlated with insulin concentration (r = 0·44, P < 0·001), although it was not correlated with plasma vitamin A concentration (r = –0·01, P > 0·05). It was concluded that dietary vitamin A in cattle does not affect plasma leptin concentration.