We conducted an isotopic analysis of groundwater in Orange County, California, USA, around the Talbert Seawater Injection Barrier to determine if recycled water, used to artificially recharge local aquifers, carries a unique isotopic signature that can be used as a tracer. From September 2014 to April 2015, we collected groundwater from six privately owned wells within the coastal groundwater basin, along with various surface waters. All water samples were analyzed for their stable isotopic composition (δ18O, δD), the δ13C and 14C signature of the dissolved inorganic carbon (DIC) pool, DIC concentration, pH, and salinity. The DIC of groundwater mixing with recycled water is enriched in 14C above natural background levels, with varying signal strength through time, depleted in δ13C, and low in DIC concentration. Water isotopes further suggest that recycled water is a mixture of Colorado River water and regional groundwater. In contrast, groundwater found further away from the injection barrier has carbon and water isotope composition consistent with regional groundwater and Santa Ana River water. Our findings imply that recycled water injected through the Talbert Barrier is isotopically unique, and that 14C enrichment may be used as an intrinsic tracer of artificial recharge within the basin.