The current study aims to make welfare-based recommendations for gun/cartridge combinations and shooting positions that will ensure death of the sheep (horned, unhorned, rams and ewes), without the need for either sticking or pithing. The study examined the pathophysiology of captive-bolt gun (CBG) injuries that result in incomplete concussion leading to death. Behavioural, brainstem and cranial/spinal responses were examined along with gross pathology in 489 animals (116 polled ewes, 134 horned ewes, 117 polled rams and 122 horned rams) following a variety of CBG-cartridge combinations. Shooting horned rams was more challenging than unhorned sheep, partly because minor movements of the head at the time of shooting can result in deflection of the gun by the horns. Marksmanship was the definitive factor: 100% of animals that showed signs of incomplete concussion were found to have been shot incorrectly. The findings will have application when it is necessary to kill sheep on farms for disease control or euthanasia purposes.

Flax seed meal (FSM) is rich in various nutrients, especially CP and energy, and can be used as animal protein feed. In animal husbandry production, it is a long-term goal to replace soybean meal (SBM) in animal feed with other plant protein feed. However, studies on the effects of replacing SBM with FSM in fattening sheep are limited. The aim of this experiment was to study the effects of replacing a portion of SBM with FSM on nutrient digestibility, rumen microbial protein synthesis and growth performance in sheep. Thirty-six Dorper × Small Thin-Tailed crossbred rams (BW = 40.4 ± 1.73 kg, mean ± SD) were randomly assigned into four groups. The dietary treatments (forage/concentrate, 45 : 55) were isocaloric according to the nutrient requirements of rams. Soybean meal was replaced with FSM at different levels (DM basis): (1) 18% SBM (18SBM), (2) 12% SBM and 6% FSM (6FSM), (3) 6% SBM and 12% FSM (12FSM) and (4) 18% FSM (18FSM). The rams were fed in individual pens for 60 days, with the first 10 days for adaptation to diets, and then the digestibility of nutrients was determined. There was no significant difference in DM intake, but quadratic (P < 0.001) effects on the average daily gain and feed efficiency were detected, with the highest values in the 6FSM and 12FSM groups. For DM and NDF digestibility, quadratic effects were observed with the higher values in the 6FSM and 12FSM groups, but the digestibility of CP linearly decreased with the increase in FSM in the diet (P = 0.043). There was a quadratic (P < 0.001) effect of FSM inclusion rate on the estimated microbial CP yield. However, the values of intestinally absorbable dietary protein decreased linearly (P < 0.001). For the supply of metabolisable protein, both the linear (P = 0.001) and quadratic (P = 0.044) effects were observed with the lowest value in the 18FSM group. Overall, the results indicated that SBM can be effectively replaced by FSM in the diets of fattening sheep and the optimal proportion was 12.0% under the conditions of this experiment.

We compared the use of recto-anal mucosal swab (RAMS) culture and faecal culture for the detection of E. coli O157 in a mob of Merino sheep. Fifty Merino wethers and maiden ewes housed in indoor pens were sampled on five occasions. We detected E coli O157 in 32% (16/50) of sheep, with weekly prevalence ranging from 4% (2/50) to 16% (8/50). Overall, 12·5% (2/16) were detected by RAMS culture only, and 37·5% (6/16) were detected by faecal culture only. The level of agreement between the two sampling methods was moderate [kappa statistic = 0·583, 95% confidence interval (CI) 0·460–0·707]. The relative sensitivities of RAMS and faecal culture were 67% (95% CI 41–86) and 57% (95% CI 34–77), respectively. We identified four super-shedding sheep using direct faecal culture. Although the majority of culture-positive sheep were detected at one sampling point only, 3/4 super-shedding sheep were culture-positive at two sampling points, and 1/4 was culture-positive at four sampling points. Persistent culture positivity may indicate sheep that could be considered ‘super-shedders' at some point. The use of immunomagnetic separation further improved the rate of detection of E. coli O157, which was isolated from 1/34 animals that were previously negative by enrichment culture alone. A significant difference between sampling weeks was detected for both faecal (P = 0·021) and RAMS (P = 0·006), with the prevalence at the mid-point of sampling (week 4) significantly (P < 0·05) higher than at the beginning or end of the study. Study conditions (penned sheep) might have been responsible for the high prevalence and the epidemic pattern of infection observed, and could serve as a future model for studies of E. coli O157 transmission, shedding and super-shedding in sheep.

Switchgrass (Panicum virgatum cv. Alamo) standing hay was cut and offered to 16 rams. The rams were randomly allocated to one of the following levels of feeding: 0·5; 1·0; 1·5 and 2·0 ✕ maintenance. The forage was offered twice a day to the animals; no supplements were given. The animals were individually penned and fitted with faeces collection bags. After 8 days for adaptation, in vivo dry matter apparent digestibility (DMD) and proportion of blades and stem + sheath of the consumed forage were measured for 8 days. DMD varied from 0·310 (0·5) to 0·391 (2·0) and the proportion of the blade fraction in the diet from 0·44 (0·5) to 0·78 (2·0). The short-term intake rate (STIR) of rams was measured for 5 days. The animals were divided randomly into four blocks, with one ram of each treatment in each block. After 4 h without food and 15 min intervals between blocks, the amount of forage consumed during 4 min of active eating was measured. The amounts consumed were (in g DM per min per 50 kg live weight): 0·5: 8·2; 1·0: 7·8; 1·5: 5·3 and 2·0: 5·3 (0·5 = 1·0 > 1·5 = 2·0; P < 0·01). The results show that offer rate of P. virgatum affects the blade: stem + sheath ratio of intake and, therefore, the nutritive value of the diet. Level of feeding before measuring STIR influences the amount of forage consumed during the test.

Sixty-four mature ewes (equal numbers of Bluefaced Leicester ♂ × Swaledale ♀ and Suffolk ♂ × (Bluefaced Leicester ♂ × Swaledale ♀) which had lambed in March were separated from their lambs on 1 fune. They were randomly allocated from breed and live-weight blocks to one of four treatments arranged in a 2 × 2 factorial design. All received vaginal implants, with or without melatonin, on 1 June. The implants were removed, all ewes given 20 mg progesterone in oil intramuscularly and equal numbers isolated or introduced to rams on 12 July. Ovarian activity was monitored by progesterone concentrations in blood taken three times weekly from the jugular vein. Four ewes receiving melatonin and four control ewes were bled for 24 h on 6 July to determine plasma melatonin concentrations.

Melatonin treatment increased plasma melatonin concentrations during the day. All ewes were anovular prior to 12 July. There were no differences between the two ewe types in their response to treatment. The number of ewes ovulating before 27 August was: control 1; melatonin 6; ram 16; melatonin + ram 16. The mean date of emulation for both the ram and melatonin + ram groups was 21 July. The number of ewes showing oestrous behaviour was 16 and 15 for the ram and melatonin + ram treatments respectively. The proportion of ewes responding to ram introduction was judged as unusually high, possibly as a result of the favourable male:female ratio. Pregnancy rate was in excess of 80% for both treatments, with resulting litter sizes of 14 for the ram and 1·8 for the melatonin + ram treatments respectively (P > 0·05).

Pre-pubertal ewe lambs were introduced to rams on 17 August (Tl), 12 September (T2) or 10 October (T3) or isolated from rams until 21 December (Tc) (no. = 13). Blood was taken for progesterone assay three times weekly (no. = 13) from 16 July. Blood was taken for LH assay at 15-min intervals for 8 h (no. = 8) at the time of the introduction of rams and subsequently at the time of further ram introductions. Blood was taken from Tc lambs at each ram introduction. Tl, T2 and T3 lambs ovulated for the first time 16 (s.e. 2·2) days earlier than Tc lambs but only T3 lambs ovulated after and apparently in response to the introduction of the rams. The mean baseline LH levels were higher in Tl, T2 and T3 lambs in comparison with Tc lambs at all sampling periods. The mean pulse frequency of LH secretion was significantly increased by the introduction of rams in T2 and T3 lambs in comparison with Tc lambs. The mean pulse frequency of LH secretion was greater in Tl, T2 and T3 lambs at the October sampling period in comparison to Tc lambs.