Field studies were conducted to determine if varying the time N fertilizer was applied would affect downy brome interference in winter wheat. Five treatments were compared: four broadcast application times of NH4NO3 at 56 kg N ha-1 during the fallow-crop cycle: 1) during fallow; 2) at planting; 3) during crop dormancy; 4) before winter wheat jointing; and 5) a control where no N was applied. Downy brome was least responsive to N applied during fallow. All N applications during the growing season of winter wheat increased downy brome biomass and culms m-2. Downy brome interference prevented winter wheat from responding positively to N. For example, when crop season precipitation was only 70% of normal, applying N reduced grain yield of winter wheat infested with downy brome from 12 to 28%. This study indicates that N manipulation offers potential for reducing downy brome interference in winter wheat.