Field trials were conducted to evaluate the effect of simulated glyphosate drift rates applied to two non-irrigated potato cultivars in the late tuber bulking stage grown for potato seed production. Mother plants receiving a sublethal glyphosate application had similar yields regardless of the year or cultivar. ‘Red LaSoda' seed pieces visually evaluated the following spring had more sprout inhibition from glyphosate-treated plots. With ‘Russet Burbank', only seed pieces from mother plants that received more than 18 g ha−1 glyphosate showed sprout inhibition. Total yield reductions the year following glyphosate treatments were attributed to fewer and smaller tubers from plants that did not emerge or were delayed in emergence. Red LaSoda seed pieces from mother plants receiving ≥ 71 g ha−1 glyphosate yielded less than the nontreated. In 2005, Russet Burbank seed pieces from mother plants receiving ≥35 g ha−1 glyphosate had reduced yields, whereas in 2006, all glyphosate treatments reduced total yield compared with the nontreated.