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Chapter 2 - Immunohistochemistry Quality Assurance and Quality Control
- By Bronwyn Christiansen, Hazel Chambers
Edited by Trung Nguyen
Book:

Immunohistochemistry

Published online:

16 June 2022

Print publication:

07 July 2022, pp 24-66
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Summary

Many parameters are associated with IHC testing assays. With so many variables, it is quite easy to accumulate errors within the system. To make things more manageable, these considerations are categorized into three main groups. Pre-analytic aspects occur before the assay, analytic factors are concerned with the staining protocol and post-analytic elements relate to interpreting of results. It has also come to reason that any one variable can impact the reliability and consistency of the overall IHC assay. In this regard, standardization requirements have been enlisted to assist laboratories achieve optimal results. In addition, monitoring proficiency testing regimens and various organizations are in place to ensure high levels of standards are attained. All these endeavours are known as quality assurance and quality control measures. They are arranged under the overall umbrella of a facility’s quality management system.