Despite the surge of interest in urban agriculture, there have been few studies that address the biophysical challenges and opportunities of food production in urban environments. This study aims to determine the relative influence of atmospheric pollutants and microclimatic factors on the physiological response and productivity of vegetable crops across an urban-to-rural latitudinal transect in the greater Chicago metropolitan region. Data collected at each of six sites include continuous measures of atmospheric pollutants and microclimatic factors, and biweekly measures of physiological response and yield of various vegetable crops and cultivars. Preliminary data collected in early 2013 suggest that there is substantial variability in environmental factors and crop yield across this urban-to-rural transect. Results of this study will provide a scientific basis for crop adaptation to the urban environment and establish practical crop and cultivar recommendations for urban and peri-urban farmers in the North Central US.