This study examines the ritual and socioeconomic significance of animals in ceremonial contexts at Kaminaljuyu, Guatemala. Kaminaljuyu was once the largest and most politically powerful highland Maya center. We compare faunal remains from different contexts, including burials and dedicatory offerings in and around monumental features, to better understand the role of animals in these deposits. We then compare ceremonial activities across Mesoamerica to identify similarities alluding to widely recognized practices. Late and Terminal Preclassic (350 BC–AD 250) ceremonies at Kaminaljuyu contain some of the earliest marine fish recovered from a Maya highland site, demonstrating the ability of early elites to obtain exotic species for special events. Dogs, including perhaps the first evidence for Preclassic hairless dogs in Guatemala, appear in deposits that resemble canine sacrificial offerings in central Mexico. The new faunal data in this study provide a novel perspective on Kaminaljuyu's sociopolitical role in the region.