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ninhydrine

Annales de Limnologie - International Journal of Limnology (1)

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1 results

HPLC and ninhydrin photometric determination of amino acids in CaCO3 rich lacustrine sediments of the Jura region, France
R. Moilleron, A. Berrekhis, A. Vidonne, F. Rémy
Journal:

Annales de Limnologie - International Journal of Limnology / Volume 32 / Issue 3 / September 1996

Published online by Cambridge University Press:

01 February 2009, pp. 197-204

Print publication:

September 1996
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A study has been carried out on the acid soluble fraction (hydrosysis at pH 1.5, 3N HCl, 20 % KCl) of organic matter (OM) in the highly calcareous lacustrine sediments of the Jura region, France. Amino acids (AA's) were quantified and determined ninhydrin photometry and HPLC respectively. There was a high degree of interference by calcium in both procedures. The mechanism of interference is explained and methodology adopted to overcome it, by removal of calcium, are dealt with. The results of both methods are compared.