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nanoflow electrospray mass spectrometry

Protein Science (1)

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Selective association of protein molecules followed by mass spectrometry
HANS VIS, CHRISTOPHER M. DOBSON, CAROL V. ROBINSON
Journal:

Protein Science / Volume 8 / Issue 6 / June 1999

Published online by Cambridge University Press:

01 June 1999, pp. 1368-1370

Print publication:

June 1999
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Nanoflow electrospray mass spectrometry was used to monitor the formation of protein heterodimers of HU proteins from Bacillus stearothermophilus and Bacillus subtilis. This has enabled us to analyze both thermodynamic and kinetic features associated with the dissociation of homodimeric HU proteins. The results obtained correlate well with the kinetics of the protein dissociation process and the free energy difference between homo- and heterodimeric species anticipated from other studies. We suggest that this approach will have general applicability in studying protein association and dissociation under near-equilibrium conditions and will be relevant to a wide range of biological systems.