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1 results

  • Myostatin gene targeting in cultured China Han ovine myoblast cells

  • L. Zhang, X. Yang, X. An, Y. Chen
  • Journal:
    animal / Volume 1 / Issue 10 / November 2007
    Published online by Cambridge University Press:
    01 November 2007, pp. 1401-1408
    Print publication:
    November 2007

  • Myostatin (MSTN), a member of the transforming growth factor-β superfamily, has been shown to be a negative regulator of myogenesis. Natural mutation in beef cattle causes double-muscling phenotypes. We report an investigation designed to knockout the MSTN gene by gene targeting in ovine myoblast cells. Two promoter-trap targeting vectors MSTN-green fluorescent protein (GFP) and MSTN-neo were constructed and used to transfect foetal and neonatal ovine primary myoblast cells. Both GFP-expressing cells and drug-resistant cells were obtained. Targeted cells expressing GFP were confirmed by polymerase chain reaction (PCR) assay and drug-resistant cells were characterised by PCR and Southern blot after growing into cell clones.