Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
  1. Home
  2. Search

Refine search

Refine search

Access:
Content type:
Publication date:
Apply   From and To filters
Subject: Show more
Journals Show more
Publishers: Show more
Societies Show more
Series: Show more
Collections: Show more

Actions for selected content:

Select all | Deselect all
  • View selected items
  • Export citations
  • Download PDF (zip)
  • Save to Kindle
  • Save to Dropbox
  • Save to Google Drive

Save Search

You can save your searches here and later view and run them again in "My saved searches".

Please provide a title, maximum of 40 characters.
Cancel
×

1 results

  • Chapter 11 - Critical Factors for Optimizing Sperm Handling and ICSI Outcomes

  • from Part III - Laboratory Handling of Retrieved Sperm
  • Edited by Ashok Agarwal, Ahmad Majzoub, Sandro C. Esteves
  • Book:
    Manual of Sperm Retrieval and Preparation in Human Assisted Reproduction
    Published online:
    09 April 2021
    Print publication:
    10 June 2021, pp 90-98

  • Summary

    This chapter covers topics including: (1) timing of sperm retrieval, particularly whether or not it should be performed in conjunction with oocyte retrieval from the female partner; (2) processing of surgically retrieved sperm for intracytoplasmic sperm injection (ICSI) –which are the most appropriate types of sperm preparation techniques in cases of epidydimal- or testicular-retrieved spermatozoa, and low or high numbers of retrieved spermatozoa; (3) selection of surgically retrieved sperm for ICSI, including methods for the selection of immotile spermatozoa, if needed, such as mechanical touch technique, hypoosmotic swelling test, chemical motility enhancers (e.g., pentoxifylline and theophylline), laser-assisted immotile sperm selection, and birefringence–polarization microscopy; (4) artificial oocyte activation, which may be useful in those selected patients who might have low fertilization potential, as well as the potential benefits of mechanical activation, electrical stimulation, and chemical activation on ICSI outcomes.