Preferences for the height of feeding troughs of seven mares were evaluated and, as awareness of surroundings when feeding is adaptive for horses, the relationship between preference responses and attention behaviours was also assessed. First, feeding troughs at four heights (0, 25, 50, and 75 cm) were provided for 16 days, and the amount of time animals ate from each height option was measured daily. These data were used to determine the preferred/non-preferred options for each individual. An overall preference for heights of 50 and 75 cm was detected, but responses showed significant individual variation The same animals were subsequently observed, when feeding from preferred (four days) or non-preferred (four days) trough heights, while their behaviours were recorded. Moving ears/head (attention behaviours) occurred more frequently when feeding from preferred heights of feeding troughs. We concluded that to ensure better welfare conditions for the mares in this study, it was important to consider the individual variation of such preferences if possible, however, by using troughs positioned at 50 or 75 cm, the general preference could be assured. Moreover, when feeding from their preferred heights, the study mares paid more attention to their surroundings than when feeding from their non-preferred height and thus it is likely that they defended their preferred options more. However, further studies are needed to evaluate the generalisability of these findings. We have demonstrated a methodology to assess mares’ preferences and provided some preliminary data on the relationship between preferences/non-preferences and the possible emotional states of mares.

The aim of this study was to evaluate whether the peripheral cytokine expression could be affected by differences in fat thickness in healthy mares during the periparturient period (last weeks of pregnancy and first weeks of lactation). At the beginning of the 11th month of gestation, 18 mares with normal body condition score (BCS; 5) were divided into two groups with low (L; ⩽12 mm; n = 12) and high (H; >12 mm; n = 6) fat thickness. Blood samples were collected every 10 days from the 11th month of gestation to 20 days post partum. Peripheral blood mononuclear cells were separated and tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1α (IL-1α), -1β (ILβ), -2 (IL-2) and -6 (IL-6) gene expressions were performed by using reverse transcriptase PCR. TNF-α and IFN-γ expressions were affected by fatness group, as the sampling period influenced the expression of all cytokines with the exception of IFN-γ. The interaction between fatness group and sampling period tended to be significant (P < 0.10) only for IL-1β, TNF-α and IL-2. In H group, compared with L group, IL-1β and IL-2 significantly (P < 0.05) increased at parturition. TNF-α peripheral expression increased from parturition to reach the peak at +10 days (P < 0.05) only in H group. In conclusion, in normal BCS periparturient mares, small body fat thickness variations influence the peripheral cytokine expression, showing a possible role for cytokines in parturition and lactation, events strongly linked to adipose tissue availability.

This study aimed at determining the effect of the increase of foraging opportunities on the behaviour and welfare of breeding mares housed in individual boxes but allowed outside 6 h a day in a bare paddock. One hundred Arab breeding mares were divided into two groups of 50 according to the treatment and allowed outside in two bare paddocks at the same density (115 mare/ha) where water and shelter were provided. The treatment consisted in providing the opportunity to forage on hay. Twenty-minute animal focal samplings and scan samplings were used to determine the time budget of the mares during the period from 0900 to 1500 h and study their social behaviour. A total of 300 focal sampling (6000 min), 3300 individual scan sampling (6000 min) and 62 group observations (1240 min) corresponding to the 100 mares were recorded. Non-parametric tests were used to analyse data. Results showed that experimental mares spent more time feeding (65.12% ± 2.40% v. 29.75% ± 2.45%, P < 0.01) and less time in locomotion (11.70% ± 1.31% v. 23.56% ± 1.34%, P < 0.01), stand resting (11.76% ± 2.57% v. 27.52% ± 2.62%, P < 0.01) and alert standing (5.23% ± 1.2% v. 14.71% ± 1.23%, P < 0.01). There was more bonding among experimental mares than control ones (26 v. 14, P < 0.05). Experimental mares showed more positive social interactions (P < 0.01) and less aggression (P < 0.01). These results suggest that giving densely housed mares foraging opportunities improves their welfare.

The effect of the stage of lactation, the number of foals and age of the mare on changes in the fat content and fatty acid composition of colostrum and milk of primitive Konik horses was investigated. Colostrum and milk samples from 12 lactating mares were collected at the beginning of lactation, on the days 1 and 2 after foaling and then, starting from the first month of lactation, at 4-week intervals up to the sixth month of lactation. Significant differences were observed in fat content as well as the composition of some analysed fatty acids between colostrum and milk of mares of the Konik breed. The number of foalings and the age of mares did not have a statistically significant effect on the fat content in milk and had only a slight effect on the fatty acid composition. Milk produced by mares of the Konik breed is characterized by a considerable content of polyene fatty acids with 18 carbon atoms, a low ratio of n-6 fatty acids to n-3 fatty acids as well as low, highly advantageous values of atherogenic and thrombogenic indices.